FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated a5.342000000085f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated a5.342000000085f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340713
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG197625.80018960239263No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA146224.291588521717692No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA106503.1257979589860088No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG94522.7741823763695543No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC70372.0653746701769524No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT66151.9415167604406054No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA59321.741054788047418No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT55581.6312849817881911No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA42451.2459166512578035No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG40471.1878032244146832No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG39461.1581595066815766No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG23750.6970676199616687No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA23310.6841535251076419No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA17200.5048237079301348No Hit
GGGGGGAGCGAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA9120.26767396606528077No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8970.2632714337286807No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC7010.20574501119710722No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA6450.18930889047380053No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC5940.1743402805293605No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT5500.1614261856753338No Hit
CGGGTTATTAGTAGAAACAAGGGGAAAGAAAAGAGTATTGTTTTCTAGAG5340.15673015118296044No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA5060.1485120908213071No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT4990.14645757573089374No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA4680.13735900890192038No Hit
GGGGGGAGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTT4440.13031495716336036No Hit
CGGGTTATTAGTAGAAACAAGGAAGAACATTCAAGATAGAGGGAAGTATG4270.1253254205152137No Hit
CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC3670.10771529116881363No Hit
CGGGTTATTAGTAGAAACAAGGTGGGGGCACCTGGGTGACTCAGTGGGTT3610.10595427823417362No Hit
GGGGGGAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA3610.10595427823417362No Hit
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA3470.10184524805334695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTAG64550.0241.96365
ATTAGTA65300.0240.122517
TATTAGT65400.0239.193426
CGGGTTA65750.0237.547531
GTTATTA65700.0237.35544
GGTTATT66200.0235.377643
TAGTAGA67000.0235.126889
GGGTTAT66400.0234.853162
TTGGGAG7850.0227.83443
GCGAAAG54050.0227.77528
GAGCGAA53800.0227.012056
CGAAAGC54600.0226.602579
GGAGCGA53950.0226.153855
AGCGAAA54300.0226.049737
GGGAGCG54200.0223.980644
AAGCGGC2850.0219.210539
TTAGTAG72100.0217.815548
GGGGAGC75850.0216.736983
GAGCGGC4550.0212.69239
AAGGGGC8250.0212.333349
Produced by FastQC (version 0.11.2)