FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated a3.342000000084f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated a3.342000000084f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323241
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG25170.7786759724168655No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG21180.6552386609371954No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG16160.4999365798274352No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT15240.4714748438471605No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG14940.46219384298402744No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC14060.4349695737855037No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA11330.35051246593099267No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA8830.2731707920715503No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA8390.25955865747228846No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC7680.23759362209620685No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG7610.23542805522814247No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7210.22305338741063171No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT6830.21129745298399646No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC6290.19459165143035692No Hit
GGGGGGAGCGAAAGCAGGGCAGAGCCAGCTCCAGTCACTGCTCTCTGGTG5990.18531065056722384No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA5520.17077041588164868No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5150.15932384815045122No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5110.15808638136870012No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT4950.15313651424169583No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4630.14323677998768722No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC4080.12622161173860988No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA4000.12374667817510773No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC3920.12127174461160559No Hit
GGGGGGAGCAAAAGCAGGGCAGAGCCAGCTCCAGTCACTGCTCTCTGGTG3600.11137201035759696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA43950.0229.94882
TTGGGAG22800.0224.583333
TCGGGAG37600.0223.497343
GAGGGGC61450.0222.672919
CGGGAGA45050.0216.72034
GATCGGG48000.0216.671881
ATTAGTA13500.0211.425937
ATTGGGA30500.0211.26232
TATTAGT13550.0208.837656
GAGCGGC16100.0208.478279
TTATTAG13500.0207.796315
ATCCGGA3750.0205.82
TCGGGAA8950.0202.569843
TTAGTAG14200.0201.866188
AGAGCGG18150.0200.454548
GAACGGG11250.0199.266661
GAGAGGG69450.0198.963297
AAGCGGC5050.0198.91099
GAGAGCG17000.0194.558847
AAGGGGC19300.0194.22289
Produced by FastQC (version 0.11.2)