FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated a2.3420000000847a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated a2.3420000000847a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390115
Sequences flagged as poor quality0
Sequence length251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG28320.7259397869858888No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG27970.7169680735167835No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT25930.6646758007254271No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC25800.6613434500083308No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG17090.43807595196288274No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG15050.38578367917152634No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC14210.3642515668456737No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA13970.35809953475257295No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA13570.34784614793073837No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA12050.30888327800776694No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11060.28350614562372634No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA10560.2706894120964331No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC10440.26761339604988277No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT9790.2509516424644015No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC6940.17789626135883008No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG6700.17174422926572933No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA6380.16354151980826165No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA5780.14816143957550978No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5260.13483203670712482No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT5250.13457570203657895No Hit
GGGGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGAC4280.10971123899363008No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4220.1081732309703549No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT4080.1045845455827128No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC4070.10432821091216693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAG7600.0222.434223
ATCCGGA8300.0221.385542
TATTAGT21700.0220.725816
ATTAGTA22350.0216.498877
TTATTAG22100.0216.176485
ATCGGGA26350.0214.781772
CGGGTTA22650.0213.090521
GGGTTAT22700.0211.541852
GATCCGG9000.0210.972211
GTTATTA22950.0208.169944
TTAGTAG23550.0204.426768
CCGGAGA8400.0204.166664
TAGTAGA23750.0203.736859
TCGGGAG23450.0203.731353
GAGCGAA56000.0201.906236
GGAGCGA55950.0201.867745
GATCGGG28950.0200.14681
CGAAAGC57850.0198.837529
AGCGAAA56950.0198.75337
GCGAAAG57600.0197.999118
Produced by FastQC (version 0.11.2)