##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE52Y l01n01 flu mrt-pcr updated h7.3410000000876d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74
Sequences flagged as poor quality	0
Sequence length	251
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.513513513513516	0.0	0.0	0.0	0.0	0.0
2	32.86486486486486	0.0	0.0	0.0	0.0	0.0
3	33.027027027027025	0.0	0.0	0.0	0.0	0.0
4	32.78378378378378	0.0	0.0	0.0	0.0	0.0
5	32.5	0.0	0.0	0.0	0.0	0.0
6	34.054054054054056	0.0	0.0	0.0	0.0	0.0
7	33.054054054054056	0.0	0.0	0.0	0.0	0.0
8	33.851351351351354	0.0	0.0	0.0	0.0	0.0
9	34.527027027027025	0.0	0.0	0.0	0.0	0.0
10-14	35.36486486486486	0.0	0.0	0.0	0.0	0.0
15-19	36.9081081081081	0.0	0.0	0.0	0.0	0.0
20-24	36.69459459459459	0.0	0.0	0.0	0.0	0.0
25-29	36.556756756756755	0.0	0.0	0.0	0.0	0.0
30-34	35.781081081081076	0.0	0.0	0.0	0.0	0.0
35-39	33.424324324324324	0.0	0.0	0.0	0.0	0.0
40-44	34.01891891891892	0.0	0.0	0.0	0.0	0.0
45-49	35.0945945945946	0.0	0.0	0.0	0.0	0.0
50-54	35.843243243243236	0.0	0.0	0.0	0.0	0.0
55-59	35.08108108108108	0.0	0.0	0.0	0.0	0.0
60-64	35.44594594594595	0.0	0.0	0.0	0.0	0.0
65-69	35.6027027027027	0.0	0.0	0.0	0.0	0.0
70-74	34.4054054054054	0.0	0.0	0.0	0.0	0.0
75-79	30.832432432432434	0.0	0.0	0.0	0.0	0.0
80-84	30.04594594594595	0.0	0.0	0.0	0.0	0.0
85-89	30.04054054054054	0.0	0.0	0.0	0.0	0.0
90-94	30.151351351351355	0.0	0.0	0.0	0.0	0.0
95-99	30.172972972972975	0.0	0.0	0.0	0.0	0.0
100-104	30.15945945945946	0.0	0.0	0.0	0.0	0.0
105-109	30.05135135135135	0.0	0.0	0.0	0.0	0.0
110-114	29.95945945945946	0.0	0.0	0.0	0.0	0.0
115-119	29.754054054054052	0.0	0.0	0.0	0.0	0.0
120-124	29.34054054054054	0.0	0.0	0.0	0.0	0.0
125-129	29.81351351351351	0.0	0.0	0.0	0.0	0.0
130-134	29.35945945945946	0.0	0.0	0.0	0.0	0.0
135-139	29.310810810810814	0.0	0.0	0.0	0.0	0.0
140-144	28.513513513513516	0.0	0.0	0.0	0.0	0.0
145-149	28.8	0.0	0.0	0.0	0.0	0.0
150-154	28.435135135135134	0.0	0.0	0.0	0.0	0.0
155-159	28.72972972972973	0.0	0.0	0.0	0.0	0.0
160-164	28.435135135135134	0.0	0.0	0.0	0.0	0.0
165-169	27.918918918918923	0.0	0.0	0.0	0.0	0.0
170-174	28.156756756756756	0.0	0.0	0.0	0.0	0.0
175-179	26.308108108108108	0.0	0.0	0.0	0.0	0.0
180-184	26.927027027027027	0.0	0.0	0.0	0.0	0.0
185-189	27.10810810810811	0.0	0.0	0.0	0.0	0.0
190-194	26.805405405405406	0.0	0.0	0.0	0.0	0.0
195-199	26.624324324324323	0.0	0.0	0.0	0.0	0.0
200-204	25.70810810810811	0.0	0.0	0.0	0.0	0.0
205-209	24.759459459459457	0.0	0.0	0.0	0.0	0.0
210-214	25.527027027027025	0.0	0.0	0.0	0.0	0.0
215-219	24.978378378378377	0.0	0.0	0.0	0.0	0.0
220-224	24.67027027027027	0.0	0.0	0.0	0.0	0.0
225-229	23.86756756756757	0.0	0.0	0.0	0.0	0.0
230-234	24.70810810810811	0.0	0.0	0.0	0.0	0.0
235-239	23.67297297297297	0.0	0.0	0.0	0.0	0.0
240-244	23.581081081081084	0.0	0.0	0.0	0.0	0.0
245-249	22.772972972972973	0.0	0.0	0.0	0.0	0.0
250-251	22.222972972972975	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1103	1	2.571428571428573
1103	2	1.571428571428573
1103	3	0.4285714285714306
1103	4	1.2857142857142847
1103	5	-0.2857142857142847
1103	6	1.2857142857142847
1103	7	1.142857142857146
1103	8	-2.4285714285714306
1103	9	1.857142857142854
1103	10-14	2.2857142857142776
1103	15-19	1.4857142857142804
1103	20-24	2.4571428571428555
1103	25-29	2.057142857142864
1103	30-34	1.4285714285714306
1103	35-39	1.971428571428568
1103	40-44	1.857142857142854
1103	45-49	1.4571428571428555
1103	50-54	2.1714285714285637
1103	55-59	1.971428571428568
1103	60-64	1.971428571428568
1103	65-69	2.714285714285708
1103	70-74	3.5142857142857196
1103	75-79	4.628571428571426
1103	80-84	2.857142857142854
1103	85-89	1.7999999999999972
1103	90-94	1.942857142857143
1103	95-99	-8.79999999999999
1103	100-104	1.6000000000000014
1103	105-109	-0.17142857142857792
1103	110-114	1.3714285714285666
1103	115-119	1.8857142857142861
1103	120-124	2.171428571428571
1103	125-129	1.2285714285714278
1103	130-134	1.971428571428575
1103	135-139	0.20000000000000284
1103	140-144	3.1999999999999957
1103	145-149	3.6285714285714263
1103	150-154	3.971428571428568
1103	155-159	4.828571428571429
1103	160-164	1.3428571428571416
1103	165-169	3.885714285714286
1103	170-174	1.942857142857143
1103	175-179	-0.7428571428571438
1103	180-184	4.5428571428571445
1103	185-189	9.171428571428567
1103	190-194	8.62857142857143
1103	195-199	7.771428571428569
1103	200-204	7.800000000000004
1103	205-209	7.428571428571434
1103	210-214	7.285714285714288
1103	215-219	3.514285714285716
1103	220-224	4.399999999999995
1103	225-229	4.771428571428572
1103	230-234	5.828571428571429
1103	235-239	5.31428571428572
1103	240-244	-1.1142857142857103
1103	245-249	3.1999999999999993
1103	250-251	4.285714285714285
2113	1	2.571428571428573
2113	2	2.571428571428573
2113	3	0.4285714285714306
2113	4	2.2857142857142847
2113	5	-1.2857142857142847
2113	6	1.2857142857142847
2113	7	-0.8571428571428541
2113	8	-1.4285714285714306
2113	9	-2.142857142857146
2113	10-14	2.085714285714282
2113	15-19	1.6857142857142833
2113	20-24	0.6571428571428584
2113	25-29	-0.5428571428571374
2113	30-34	-1.1714285714285708
2113	35-39	1.7714285714285722
2113	40-44	1.857142857142854
2113	45-49	1.4571428571428555
2113	50-54	2.1714285714285637
2113	55-59	1.7714285714285722
2113	60-64	0.971428571428568
2113	65-69	-1.085714285714289
2113	70-74	0.7142857142857153
2113	75-79	-2.771428571428576
2113	80-84	1.4571428571428555
2113	85-89	0.3999999999999986
2113	90-94	-2.2571428571428527
2113	95-99	2.20000000000001
2113	100-104	1.4000000000000057
2113	105-109	0.028571428571417812
2113	110-114	1.5714285714285694
2113	115-119	-3.3142857142857167
2113	120-124	0.5714285714285694
2113	125-129	-1.1714285714285708
2113	130-134	-1.8285714285714292
2113	135-139	-1.1999999999999957
2113	140-144	2.0
2113	145-149	2.028571428571425
2113	150-154	3.971428571428568
2113	155-159	3.6285714285714263
2113	160-164	1.942857142857143
2113	165-169	4.4857142857142875
2113	170-174	0.9428571428571431
2113	175-179	5.857142857142858
2113	180-184	5.142857142857146
2113	185-189	7.37142857142857
2113	190-194	7.62857142857143
2113	195-199	7.9714285714285715
2113	200-204	6.800000000000004
2113	205-209	8.828571428571433
2113	210-214	6.685714285714287
2113	215-219	6.914285714285715
2113	220-224	4.9999999999999964
2113	225-229	3.5714285714285694
2113	230-234	5.828571428571429
2113	235-239	4.514285714285716
2113	240-244	7.085714285714289
2113	245-249	6.9999999999999964
2113	250-251	3.2857142857142847
2102	1	2.571428571428573
2102	2	2.571428571428573
2102	3	0.4285714285714306
2102	4	2.2857142857142847
2102	5	0.7142857142857153
2102	6	-0.7142857142857153
2102	7	-0.8571428571428541
2102	8	-1.4285714285714306
2102	9	-2.142857142857146
2102	10-14	0.48571428571428044
2102	15-19	1.6857142857142833
2102	20-24	2.4571428571428555
2102	25-29	2.4571428571428626
2102	30-34	2.4285714285714306
2102	35-39	1.971428571428568
2102	40-44	3.2571428571428527
2102	45-49	2.6571428571428513
2102	50-54	2.771428571428565
2102	55-59	2.171428571428571
2102	60-64	1.971428571428568
2102	65-69	3.1142857142857068
2102	70-74	3.7142857142857153
2102	75-79	4.028571428571425
2102	80-84	3.057142857142857
2102	85-89	2.0
2102	90-94	2.5428571428571445
2102	95-99	3.6000000000000085
2102	100-104	3.200000000000003
2102	105-109	2.2285714285714207
2102	110-114	3.5714285714285694
2102	115-119	2.885714285714286
2102	120-124	2.5714285714285694
2102	125-129	1.6285714285714263
2102	130-134	2.5714285714285694
2102	135-139	0.8000000000000043
2102	140-144	4.799999999999997
2102	145-149	4.028571428571425
2102	150-154	5.771428571428565
2102	155-159	5.628571428571426
2102	160-164	7.74285714285714
2102	165-169	6.885714285714286
2102	170-174	7.5428571428571445
2102	175-179	8.457142857142852
2102	180-184	9.342857142857142
2102	185-189	8.37142857142857
2102	190-194	7.228571428571431
2102	195-199	8.971428571428572
2102	200-204	7.800000000000004
2102	205-209	7.028571428571436
2102	210-214	2.2857142857142883
2102	215-219	7.514285714285716
2102	220-224	6.599999999999991
2102	225-229	5.171428571428571
2102	230-234	5.828571428571429
2102	235-239	6.114285714285717
2102	240-244	7.085714285714289
2102	245-249	7.199999999999999
2102	250-251	10.785714285714285
1114	1	2.571428571428573
1114	2	3.571428571428573
1114	3	1.4285714285714306
1114	4	4.285714285714285
1114	5	0.7142857142857153
1114	6	0.2857142857142847
1114	7	0.1428571428571459
1114	8	2.5714285714285694
1114	9	1.857142857142854
1114	10-14	2.885714285714279
1114	15-19	1.6857142857142833
1114	20-24	2.4571428571428555
1114	25-29	2.4571428571428626
1114	30-34	2.2285714285714278
1114	35-39	2.171428571428571
1114	40-44	3.4571428571428555
1114	45-49	2.857142857142854
1114	50-54	2.771428571428565
1114	55-59	2.971428571428568
1114	60-64	1.7714285714285722
1114	65-69	2.714285714285708
1114	70-74	4.314285714285717
1114	75-79	5.028571428571425
1114	80-84	3.6571428571428513
1114	85-89	3.0
1114	90-94	2.5428571428571445
1114	95-99	3.6000000000000085
1114	100-104	3.200000000000003
1114	105-109	2.2285714285714207
1114	110-114	3.5714285714285694
1114	115-119	2.885714285714286
1114	120-124	2.5714285714285694
1114	125-129	1.4285714285714235
1114	130-134	2.171428571428571
1114	135-139	0.6000000000000014
1114	140-144	-5.600000000000001
1114	145-149	-13.571428571428577
1114	150-154	-14.42857142857143
1114	155-159	-10.171428571428574
1114	160-164	-12.857142857142861
1114	165-169	-17.314285714285713
1114	170-174	-9.857142857142854
1114	175-179	-17.542857142857144
1114	180-184	-10.057142857142857
1114	185-189	-12.82857142857143
1114	190-194	-8.971428571428572
1114	195-199	-6.0285714285714285
1114	200-204	-5.599999999999994
1114	205-209	-10.371428571428567
1114	210-214	0.28571428571428825
1114	215-219	1.3142857142857132
1114	220-224	6.399999999999995
1114	225-229	5.171428571428571
1114	230-234	5.028571428571432
1114	235-239	7.114285714285717
1114	240-244	4.885714285714286
1114	245-249	0.7999999999999972
1114	250-251	-3.2142857142857153
1108	1	1.571428571428573
1108	2	1.571428571428573
1108	3	0.4285714285714306
1108	4	2.2857142857142847
1108	5	-0.2857142857142847
1108	6	-1.7142857142857153
1108	7	-0.8571428571428541
1108	8	2.5714285714285694
1108	9	1.857142857142854
1108	10-14	2.885714285714279
1108	15-19	1.2857142857142847
1108	20-24	-1.7428571428571402
1108	25-29	0.057142857142864045
1108	30-34	2.028571428571432
1108	35-39	2.171428571428571
1108	40-44	2.6571428571428584
1108	45-49	2.6571428571428513
1108	50-54	1.5714285714285623
1108	55-59	2.5714285714285694
1108	60-64	0.971428571428568
1108	65-69	3.3142857142857096
1108	70-74	3.5142857142857196
1108	75-79	4.028571428571425
1108	80-84	3.857142857142854
1108	85-89	2.6000000000000014
1108	90-94	2.142857142857146
1108	95-99	2.8000000000000114
1108	100-104	3.200000000000003
1108	105-109	1.6285714285714192
1108	110-114	2.971428571428568
1108	115-119	1.8857142857142861
1108	120-124	1.971428571428568
1108	125-129	1.028571428571425
1108	130-134	1.971428571428575
1108	135-139	0.4000000000000057
1108	140-144	3.799999999999997
1108	145-149	4.028571428571425
1108	150-154	3.971428571428568
1108	155-159	0.6285714285714263
1108	160-164	7.542857142857137
1108	165-169	6.885714285714286
1108	170-174	7.5428571428571445
1108	175-179	8.657142857142855
1108	180-184	9.342857142857142
1108	185-189	9.37142857142857
1108	190-194	8.62857142857143
1108	195-199	9.171428571428574
1108	200-204	8.600000000000001
1108	205-209	10.62857142857143
1108	210-214	8.285714285714288
1108	215-219	8.314285714285713
1108	220-224	6.799999999999994
1108	225-229	5.371428571428567
1108	230-234	6.828571428571429
1108	235-239	7.114285714285717
1108	240-244	8.085714285714289
1108	245-249	8.999999999999996
1108	250-251	10.285714285714285
2105	1	-14.428571428571427
2105	2	-14.428571428571427
2105	3	-3.5714285714285694
2105	4	-14.714285714285715
2105	5	-0.2857142857142847
2105	6	-3.7142857142857153
2105	7	-1.857142857142854
2105	8	-2.4285714285714306
2105	9	-3.142857142857146
2105	10-14	-12.714285714285719
2105	15-19	-9.514285714285716
2105	20-24	-8.742857142857144
2105	25-29	-8.942857142857136
2105	30-34	-9.37142857142857
2105	35-39	-11.82857142857143
2105	40-44	-15.742857142857144
2105	45-49	-13.342857142857145
2105	50-54	-13.828571428571436
2105	55-59	-14.028571428571432
2105	60-64	-9.028571428571428
2105	65-69	-12.88571428571429
2105	70-74	-19.685714285714283
2105	75-79	-19.571428571428577
2105	80-84	-17.942857142857147
2105	85-89	-12.0
2105	90-94	-9.257142857142856
2105	95-99	-5.79999999999999
2105	100-104	-14.599999999999998
2105	105-109	-7.771428571428579
2105	110-114	-16.42857142857143
2105	115-119	-8.914285714285715
2105	120-124	-12.228571428571431
2105	125-129	-5.771428571428572
2105	130-134	-9.228571428571428
2105	135-139	-1.5999999999999943
2105	140-144	-12.400000000000002
2105	145-149	-3.3714285714285737
2105	150-154	-8.628571428571433
2105	155-159	-7.371428571428574
2105	160-164	-12.857142857142861
2105	165-169	-9.114285714285714
2105	170-174	-10.857142857142854
2105	175-179	0.6571428571428548
2105	180-184	-8.657142857142855
2105	185-189	-8.62857142857143
2105	190-194	-10.37142857142857
2105	195-199	-12.82857142857143
2105	200-204	-11.199999999999996
2105	205-209	-11.571428571428566
2105	210-214	-10.914285714285715
2105	215-219	-11.485714285714284
2105	220-224	-11.800000000000008
2105	225-229	-9.62857142857143
2105	230-234	-12.17142857142857
2105	235-239	-13.285714285714281
2105	240-244	-12.31428571428571
2105	245-249	-14.200000000000003
2105	250-251	-13.214285714285715
2109	1	2.571428571428573
2109	2	2.571428571428573
2109	3	0.4285714285714306
2109	4	2.2857142857142847
2109	5	0.7142857142857153
2109	6	3.2857142857142847
2109	7	3.142857142857146
2109	8	2.5714285714285694
2109	9	1.857142857142854
2109	10-14	2.085714285714282
2109	15-19	1.6857142857142833
2109	20-24	2.4571428571428555
2109	25-29	2.4571428571428626
2109	30-34	2.4285714285714306
2109	35-39	1.7714285714285722
2109	40-44	2.6571428571428584
2109	45-49	2.2571428571428527
2109	50-54	2.3714285714285666
2109	55-59	2.5714285714285694
2109	60-64	1.3714285714285737
2109	65-69	2.1142857142857068
2109	70-74	3.914285714285718
2109	75-79	4.628571428571426
2109	80-84	3.057142857142857
2109	85-89	2.200000000000003
2109	90-94	2.3428571428571416
2109	95-99	2.4000000000000057
2109	100-104	2.0
2109	105-109	1.828571428571422
2109	110-114	3.3714285714285666
2109	115-119	2.6857142857142833
2109	120-124	2.3714285714285666
2109	125-129	1.6285714285714263
2109	130-134	2.3714285714285737
2109	135-139	0.8000000000000043
2109	140-144	4.199999999999996
2109	145-149	3.2285714285714278
2109	150-154	5.371428571428567
2109	155-159	2.828571428571429
2109	160-164	7.142857142857139
2109	165-169	4.285714285714285
2109	170-174	2.7428571428571473
2109	175-179	-5.342857142857145
2109	180-184	-9.657142857142855
2109	185-189	-12.82857142857143
2109	190-194	-12.771428571428569
2109	195-199	-15.028571428571428
2109	200-204	-14.199999999999996
2109	205-209	-11.971428571428568
2109	210-214	-13.914285714285715
2109	215-219	-16.085714285714285
2109	220-224	-17.400000000000006
2109	225-229	-14.42857142857143
2109	230-234	-17.17142857142857
2109	235-239	-16.885714285714283
2109	240-244	-13.714285714285712
2109	245-249	-13.000000000000004
2109	250-251	-12.214285714285715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	3.0
22	8.0
23	6.0
24	6.0
25	5.0
26	2.0
27	1.0
28	2.0
29	5.0
30	2.0
31	1.0
32	5.0
33	3.0
34	1.0
35	2.0
36	11.0
37	8.0
38	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.702702702702695	24.324324324324326	2.7027027027027026	20.27027027027027
2	28.37837837837838	50.0	17.56756756756757	4.054054054054054
3	32.432432432432435	8.108108108108109	51.35135135135135	8.108108108108109
4	35.13513513513514	10.81081081081081	9.45945945945946	44.5945945945946
5	51.35135135135135	17.56756756756757	24.324324324324326	6.756756756756757
6	58.108108108108105	10.81081081081081	22.972972972972975	8.108108108108109
7	9.45945945945946	68.91891891891892	16.216216216216218	5.405405405405405
8	18.91891891891892	51.35135135135135	22.972972972972975	6.756756756756757
9	47.2972972972973	9.45945945945946	18.91891891891892	24.324324324324326
10-14	22.162162162162165	38.648648648648646	14.324324324324325	24.864864864864867
15-19	19.45945945945946	29.45945945945946	20.54054054054054	30.54054054054054
20-24	24.864864864864867	32.7027027027027	23.513513513513516	18.91891891891892
25-29	17.027027027027028	24.054054054054056	32.16216216216216	26.756756756756754
30-34	13.783783783783784	30.810810810810814	25.405405405405407	30.0
35-39	27.027027027027028	29.72972972972973	25.405405405405407	17.83783783783784
40-44	18.37837837837838	29.72972972972973	26.756756756756754	25.135135135135133
45-49	20.81081081081081	26.216216216216214	32.16216216216216	20.81081081081081
50-54	31.62162162162162	15.675675675675677	25.405405405405407	27.297297297297295
55-59	11.891891891891893	17.2972972972973	41.08108108108108	29.72972972972973
60-64	21.891891891891895	23.513513513513516	34.054054054054056	20.54054054054054
65-69	17.2972972972973	49.72972972972973	20.0	12.972972972972974
70-74	12.972972972972974	54.054054054054056	17.2972972972973	15.675675675675677
75-79	13.243243243243244	40.54054054054054	23.243243243243246	22.972972972972975
80-84	21.35135135135135	35.13513513513514	24.054054054054056	19.45945945945946
85-89	20.0	28.10810810810811	25.135135135135133	26.756756756756754
90-94	18.91891891891892	31.891891891891895	24.864864864864867	24.324324324324326
95-99	18.10810810810811	28.37837837837838	29.72972972972973	23.783783783783786
100-104	22.972972972972975	27.837837837837835	22.162162162162165	27.027027027027028
105-109	20.81081081081081	24.324324324324326	25.405405405405407	29.45945945945946
110-114	17.027027027027028	25.675675675675674	35.13513513513514	22.162162162162165
115-119	18.37837837837838	31.351351351351354	28.91891891891892	21.35135135135135
120-124	15.405405405405407	30.0	27.837837837837835	26.756756756756754
125-129	18.37837837837838	28.91891891891892	28.10810810810811	24.594594594594597
130-134	20.0	30.0	26.486486486486488	23.513513513513516
135-139	20.81081081081081	30.54054054054054	24.594594594594597	24.054054054054056
140-144	17.83783783783784	31.891891891891895	25.135135135135133	25.135135135135133
145-149	20.27027027027027	28.10810810810811	24.594594594594597	27.027027027027028
150-154	17.56756756756757	29.72972972972973	23.243243243243246	29.45945945945946
155-159	18.37837837837838	28.37837837837838	28.91891891891892	24.324324324324326
160-164	16.486486486486488	32.16216216216216	25.405405405405407	25.945945945945947
165-169	17.56756756756757	26.486486486486488	26.486486486486488	29.45945945945946
170-174	16.486486486486488	28.91891891891892	28.10810810810811	26.486486486486488
175-179	18.64864864864865	28.91891891891892	25.945945945945947	26.486486486486488
180-184	15.945945945945947	25.405405405405407	28.64864864864865	30.0
185-189	19.18918918918919	24.864864864864867	28.10810810810811	27.837837837837835
190-194	14.864864864864865	29.72972972972973	27.56756756756757	27.837837837837835
195-199	16.486486486486488	30.270270270270274	28.37837837837838	24.864864864864867
200-204	18.37837837837838	31.351351351351354	23.783783783783786	26.486486486486488
205-209	19.18918918918919	28.64864864864865	27.027027027027028	25.135135135135133
210-214	17.56756756756757	27.837837837837835	29.18918918918919	25.405405405405407
215-219	17.2972972972973	31.62162162162162	28.64864864864865	22.432432432432435
220-224	17.2972972972973	25.135135135135133	26.756756756756754	30.810810810810814
225-229	17.027027027027028	29.72972972972973	25.675675675675674	27.56756756756757
230-234	18.10810810810811	27.027027027027028	27.027027027027028	27.837837837837835
235-239	16.486486486486488	32.16216216216216	23.243243243243246	28.10810810810811
240-244	18.64864864864865	31.351351351351354	23.513513513513516	26.486486486486488
245-249	15.675675675675677	31.351351351351354	26.756756756756754	26.216216216216214
250-251	16.216216216216218	29.054054054054053	25.675675675675674	29.054054054054053
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.3333333333333333
31	1.0
32	0.6666666666666666
33	0.3333333333333333
34	0.6666666666666666
35	1.3333333333333333
36	1.0
37	0.3333333333333333
38	1.0
39	1.0
40	0.6666666666666666
41	1.3333333333333333
42	4.0
43	4.999999999999999
44	3.3333333333333335
45	1.9999999999999998
46	3.0
47	1.6666666666666665
48	0.6666666666666666
49	1.0
50	0.6666666666666666
51	1.6666666666666665
52	1.6666666666666665
53	0.3333333333333333
54	0.3333333333333333
55	0.0
56	0.3333333333333333
57	0.6666666666666666
58	0.0
59	0.0
60	0.3333333333333333
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	74.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.24324324324324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.20289855072464	87.83783783783784
2	4.3478260869565215	8.108108108108109
3	1.4492753623188406	4.054054054054054
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT	3	4.054054054054054	TruSeq Adapter, Index 19 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	2	2.7027027027027026	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTACTCCGGAGAATCTCGTATG	2	2.7027027027027026	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	2	2.7027027027027026	No Hit
CGGGTTATTAGTAGAAACAAGGGCTTATAGATGTGTATTTACTAAGTACC	1	1.3513513513513513	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	1	1.3513513513513513	No Hit
TTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTT	1	1.3513513513513513	No Hit
ATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTC	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAATTACTCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCTCCGGAGAATCTCGTATGCC	1	1.3513513513513513	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCT	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAAACTATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 22 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGGAGTTTTTTGGCCAGAATTGATAGCAAA	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGACTCTATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 15 (97% over 36bp)
CTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATTACA	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAAATCCAGTCACATTACTCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTGACATTACTCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGACCTCCAGTCACAGAAGCTAATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 6 (97% over 36bp)
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT	1	1.3513513513513513	No Hit
ATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCTCTTC	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGCACGAATTCGTATCTCGTATG	1	1.3513513513513513	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGGCAAAATCTGTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGG	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAATAGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCACATTACTCGATATCGTATG	1	1.3513513513513513	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CTTTTGATGAGAGAAGAAATAAATACCTAGAAGAGCATCCCAGTGCTGGG	1	1.3513513513513513	No Hit
GGGGGGAGCAAAAGCAGGGGGAGTGGGTGAGGAAGCAGGCTTCCCTCTGA	1	1.3513513513513513	No Hit
AGGAAATACCAATCAACAAAAGGCATCCGCAGGCCAGATCAGTGTGCAGC	1	1.3513513513513513	No Hit
GATCGGAAGAGCACCGTCTGAACTCCATCACATTCAGAAATCTCGTATGC	1	1.3513513513513513	No Hit
AGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGA	1	1.3513513513513513	No Hit
ATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCA	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACGCTATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 7 (97% over 37bp)
ATCATCAGCCAAGTGCCCACGCTCTGCAAGTCTGCAGAGATTGCTGGGTT	1	1.3513513513513513	No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTACTCGATATCGTATGC	1	1.3513513513513513	TruSeq Adapter, Index 10 (97% over 35bp)
GACAAGGGTAGCTCCATCTGTGTGCTGTTTGAGGGAAGTAAAGGAGGCTA	1	1.3513513513513513	No Hit
AAACAGAAGCTGCAGTATGAGGGCATTTTCATCAAGGATTCCAACTCATT	1	1.3513513513513513	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1	1.3513513513513513	No Hit
CGGGTTATTAGTAGAAACAAGGGCGTTTTTTATCATTTCATCACTAATAG	1	1.3513513513513513	No Hit
CTAGTTTCTCTACCTCCGCATCCTTGCCAAGGCCTGCTGGGCCTTCCCTC	1	1.3513513513513513	No Hit
ACTCTCATCTTTTTCTTTAATACTCATAAAGGATTTGCAAATGTCTGGAG	1	1.3513513513513513	No Hit
AATTACTCCAAAACAGCCAAGTGGTCAGCCTGATGAGACCAAATGAAAAC	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGACGCTATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 20 (97% over 38bp)
GGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAAC	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACGCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 1 (97% over 39bp)
AGCATCTGCTCCTTTTCTACCAGTGCTCCCAGTTCAGCAGCTCAGAGACG	1	1.3513513513513513	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	1	1.3513513513513513	No Hit
GTATCTATCTGGAAGCATCCGACTTTCAAAACAACCAGGAGTTGCAGGCT	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGAGCAATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 12 (97% over 37bp)
GGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCA	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAAGGTATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAATCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 1 (97% over 37bp)
AATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACA	1	1.3513513513513513	No Hit
ATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATGGTGTTTGGATAGGG	1	1.3513513513513513	No Hit
GATCGGAAGAGACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCC	1	1.3513513513513513	TruSeq Adapter, Index 6 (95% over 22bp)
CTGGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCT	1	1.3513513513513513	No Hit
AGCATTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAATGA	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGACTCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 1 (97% over 38bp)
GGCGAAGATGCAACAGCAGGTCTTACTCATATCATGATTTGGCATTCCAA	1	1.3513513513513513	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	1	1.3513513513513513	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1	1.3513513513513513	No Hit
CATGGACTCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAGGA	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATATCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 19 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTTTGGGAGATCATCAAGAACACAAATCCAGT	1	1.3513513513513513	No Hit
CCCATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAG	1	1.3513513513513513	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGCGATCTCGTAT	1	1.3513513513513513	TruSeq Adapter, Index 19 (97% over 38bp)
TTTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGA	1	1.3513513513513513	No Hit
ATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCAT	1	1.3513513513513513	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0	0.0	0.0
15-19	0.0	0.0	0.0
20-24	0.0	0.0	0.0
25-29	0.0	0.0	0.0
30-34	0.0	0.0	0.0
35-39	0.0	0.0	0.0
40-44	0.0	0.0	0.0
45-49	0.0	0.0	0.0
50-54	0.0	0.0	0.0
55-59	0.0	0.0	0.0
60-64	0.0	0.0	0.0
65-69	0.0	0.0	0.0
70-74	0.0	0.0	0.0
75-79	0.5405405405405406	0.0	0.0
80-84	1.3513513513513513	0.0	0.0
85-89	1.3513513513513513	0.0	0.0
90-94	1.3513513513513513	0.0	0.0
95-99	1.3513513513513513	0.0	0.0
100-104	1.3513513513513513	0.0	0.0
105-109	1.3513513513513513	0.0	0.0
110-114	1.3513513513513513	0.0	0.0
115-119	1.3513513513513513	0.0	0.0
120-124	1.6216216216216217	0.0	0.0
125-129	3.5135135135135136	0.0	0.0
130-134	5.405405405405405	0.0	0.0
135-139	7.837837837837839	0.0	0.0
140-144	9.45945945945946	0.0	0.0
145-149	10.27027027027027	0.0	0.0
150-154	11.621621621621621	0.0	0.0
155-159	12.162162162162161	0.0	0.0
160-164	12.972972972972974	0.0	0.0
165-169	14.054054054054054	0.0	0.0
170-174	16.216216216216218	0.0	0.0
175-179	16.486486486486488	0.0	0.0
180-184	17.56756756756757	0.0	0.0
185-189	18.37837837837838	0.0	0.0
190-194	20.000000000000004	0.0	0.0
195-199	20.54054054054054	0.0	0.0
200-204	22.7027027027027	0.0	0.0
205-209	26.216216216216218	0.0	0.0
210-214	29.189189189189186	0.0	0.0
215-219	29.72972972972973	0.0	0.0
220-224	29.72972972972973	0.0	0.0
225-229	30.810810810810814	0.0	0.0
230-234	31.081081081081084	0.0	0.0
235-239	31.891891891891895	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTAT	5	0.0	245.00002	2
ATTAGTA	5	0.0	245.00002	7
TTATTAG	5	0.0	245.00002	5
GGTTATT	5	0.0	245.00002	3
CGGGTTA	5	0.0	245.00002	1
TAGTAGA	5	0.0	245.00002	9
GAATTCG	5	0.0	245.00002	245
TATTAGT	5	0.0	245.00002	6
GTTATTA	5	0.0	245.00002	4
TTAGTAG	5	0.0	245.00002	8
>>END_MODULE