FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated h7.3410000000876d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated h7.3410000000876d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT34.054054054054054TruSeq Adapter, Index 19 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC22.7027027027027026No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTACTCCGGAGAATCTCGTATG22.7027027027027026Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG22.7027027027027026No Hit
CGGGTTATTAGTAGAAACAAGGGCTTATAGATGTGTATTTACTAAGTACC11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11.3513513513513513No Hit
TTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTT11.3513513513513513No Hit
ATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAATTACTCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCTCCGGAGAATCTCGTATGCC11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCT11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAAACTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 22 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGGAGTTTTTTGGCCAGAATTGATAGCAAA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGACTCTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 15 (97% over 36bp)
CTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATTACA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAAATCCAGTCACATTACTCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTGACATTACTCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGACCTCCAGTCACAGAAGCTAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 6 (97% over 36bp)
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT11.3513513513513513No Hit
ATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCTCTTC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGCACGAATTCGTATCTCGTATG11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGGCAAAATCTGTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGG11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAATAGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCACATTACTCGATATCGTATG11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CTTTTGATGAGAGAAGAAATAAATACCTAGAAGAGCATCCCAGTGCTGGG11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGGGGAGTGGGTGAGGAAGCAGGCTTCCCTCTGA11.3513513513513513No Hit
AGGAAATACCAATCAACAAAAGGCATCCGCAGGCCAGATCAGTGTGCAGC11.3513513513513513No Hit
GATCGGAAGAGCACCGTCTGAACTCCATCACATTCAGAAATCTCGTATGC11.3513513513513513No Hit
AGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGA11.3513513513513513No Hit
ATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACGCTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 7 (97% over 37bp)
ATCATCAGCCAAGTGCCCACGCTCTGCAAGTCTGCAGAGATTGCTGGGTT11.3513513513513513No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTACTCGATATCGTATGC11.3513513513513513TruSeq Adapter, Index 10 (97% over 35bp)
GACAAGGGTAGCTCCATCTGTGTGCTGTTTGAGGGAAGTAAAGGAGGCTA11.3513513513513513No Hit
AAACAGAAGCTGCAGTATGAGGGCATTTTCATCAAGGATTCCAACTCATT11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGGCGTTTTTTATCATTTCATCACTAATAG11.3513513513513513No Hit
CTAGTTTCTCTACCTCCGCATCCTTGCCAAGGCCTGCTGGGCCTTCCCTC11.3513513513513513No Hit
ACTCTCATCTTTTTCTTTAATACTCATAAAGGATTTGCAAATGTCTGGAG11.3513513513513513No Hit
AATTACTCCAAAACAGCCAAGTGGTCAGCCTGATGAGACCAAATGAAAAC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGACGCTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 20 (97% over 38bp)
GGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAAC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACGCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 1 (97% over 39bp)
AGCATCTGCTCCTTTTCTACCAGTGCTCCCAGTTCAGCAGCTCAGAGACG11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11.3513513513513513No Hit
GTATCTATCTGGAAGCATCCGACTTTCAAAACAACCAGGAGTTGCAGGCT11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGAGCAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 12 (97% over 37bp)
GGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAAGGTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAATCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 1 (97% over 37bp)
AATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACA11.3513513513513513No Hit
ATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATGGTGTTTGGATAGGG11.3513513513513513No Hit
GATCGGAAGAGACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCC11.3513513513513513TruSeq Adapter, Index 6 (95% over 22bp)
CTGGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCT11.3513513513513513No Hit
AGCATTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAATGA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGACTCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 1 (97% over 38bp)
GGCGAAGATGCAACAGCAGGTCTTACTCATATCATGATTTGGCATTCCAA11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA11.3513513513513513No Hit
CATGGACTCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAGGA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATATCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTTTGGGAGATCATCAAGAACACAAATCCAGT11.3513513513513513No Hit
CCCATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAG11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
TTTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGA11.3513513513513513No Hit
ATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCAT11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT50.0245.000022
ATTAGTA50.0245.000027
TTATTAG50.0245.000025
GGTTATT50.0245.000023
CGGGTTA50.0245.000021
TAGTAGA50.0245.000029
GAATTCG50.0245.00002245
TATTAGT50.0245.000026
GTTATTA50.0245.000024
TTAGTAG50.0245.000028
Produced by FastQC (version 0.11.2)