FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated h6.341000000086e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated h6.341000000086e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560195
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT533309.51989932077223TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG178193.180856665982381No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT109101.9475361258133328No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA106271.897018002659788No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC89871.6042628013459597No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA85861.5326805844393472No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG68971.2311784289399228No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT63851.1397816831638983No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA61491.0976534956577622No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC56031.0001874347325486No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG46340.8272119529806585No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA42560.7597354492632029No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG41170.7349226608591651No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA37310.6660180829889591No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA35270.6296021920938245No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT34840.6219262935227912No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG28450.5078588705718544No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC27310.48750881389516154No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG25160.44912932103999503No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA21300.3802247431697891No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA15880.28347271932095075No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC15630.2790099875936058No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA14450.2579458938405377No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA13740.24527173573487804No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA5810.10371388534349646No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGCATCTCGTAT5640.1006792277689019TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA73050.0244.664611
GGGTTAT73600.0242.836292
GGGGGGA59800.0242.746641
TATTAGT75100.0237.986046
GGTTATT75450.0236.882053
TTATTAG75400.0236.876655
ATTAGTA76050.0234.85217
TAGTAGA77800.0229.411949
GTTATTA79050.0225.939294
GGGGGAG65200.0222.4542
TTAGTAG83300.0214.411768
GGAGCGA43650.0212.726245
GAGCGAA44100.0210.833346
GCGAAAG44450.0208.897638
CGAAAGC44700.0207.729319
AGCGAAA44900.0207.076847
GGGGAGC71750.0201.804893
GGGAGCG47850.0193.798344
GAGCAAA30750.0170.504066
ACGCGTA150.004966287163.33333245
Produced by FastQC (version 0.11.2)