##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE52Y l01n01 flu mrt-pcr updated h4.341000000085e6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17465 Sequences flagged as poor quality 0 Sequence length 251 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83011737761237 33.0 32.0 34.0 32.0 34.0 2 33.12608073289436 33.0 33.0 34.0 32.0 35.0 3 33.25342112797023 33.0 33.0 34.0 32.0 35.0 4 33.25983395362153 34.0 33.0 34.0 32.0 35.0 5 33.059375894646436 33.0 33.0 34.0 32.0 35.0 6 35.19387346120813 37.0 34.0 37.0 33.0 37.0 7 35.201259662181506 37.0 34.0 37.0 33.0 37.0 8 35.5456054967077 37.0 34.0 37.0 33.0 37.0 9 35.493959347265964 37.0 34.0 37.0 33.0 37.0 10-14 36.33147437732608 37.4 36.0 37.4 34.6 37.4 15-19 37.351995419410244 38.0 38.0 38.0 37.0 38.0 20-24 37.53442885771543 38.4 38.0 38.4 37.0 38.4 25-29 37.909544803893496 39.0 38.0 39.0 37.0 39.0 30-34 37.9139421700544 39.0 38.0 39.0 37.0 39.0 35-39 37.785903235041516 39.0 38.0 39.0 37.0 39.0 40-44 37.81193243630118 39.0 38.0 39.0 37.0 39.0 45-49 37.84894360148869 39.0 38.0 39.0 37.0 39.0 50-54 37.87773260807329 39.0 38.0 39.0 37.0 39.0 55-59 37.84906956770684 39.0 38.0 39.0 37.0 39.0 60-64 37.85856283996565 39.0 38.0 39.0 37.0 39.0 65-69 37.86667048382479 39.0 38.0 39.0 37.0 39.0 70-74 37.76609218436874 39.0 38.0 39.0 36.8 39.0 75-79 37.34836530203263 39.0 38.0 39.0 36.0 39.0 80-84 37.289791010592616 39.0 38.0 39.0 36.0 39.0 85-89 37.25674205553965 39.0 38.0 39.0 36.0 39.0 90-94 37.1564614944174 39.0 38.0 39.0 35.6 39.0 95-99 37.27600343544232 39.0 38.0 39.0 36.0 39.0 100-104 37.14517034068136 39.0 38.0 39.0 35.4 39.0 105-109 37.098906384196965 39.0 38.0 39.0 35.2 39.0 110-114 37.144677927283134 39.0 38.0 39.0 35.8 39.0 115-119 36.98859433152019 39.0 38.0 39.0 35.0 39.0 120-124 36.55869453192098 39.0 37.6 39.0 31.2 39.0 125-129 37.04771829373032 39.0 38.0 39.0 35.0 39.0 130-134 36.87537360435156 39.0 38.0 39.0 34.6 39.0 135-139 36.73512739765245 38.8 38.0 39.0 33.8 39.0 140-144 36.601820784425996 39.0 38.0 39.0 33.0 39.0 145-149 36.63167477812768 39.0 37.6 39.0 33.2 39.0 150-154 36.4216661895219 38.4 37.0 39.0 32.4 39.0 155-159 36.30475808760378 38.6 37.0 39.0 31.4 39.0 160-164 36.372745490981956 38.6 37.0 39.0 32.8 39.0 165-169 36.25160034354423 38.6 37.0 39.0 32.2 39.0 170-174 36.23511022044089 38.4 37.0 39.0 31.8 39.0 175-179 35.997698253650164 38.2 37.0 39.0 28.8 39.0 180-184 35.60412253077583 38.0 37.0 39.0 24.8 39.0 185-189 35.79956484397367 38.0 37.0 39.0 27.0 39.0 190-194 35.641030632693955 38.0 37.0 39.0 26.0 39.0 195-199 35.35303750357859 38.0 37.0 39.0 25.0 39.0 200-204 35.235304895505294 38.0 37.0 39.0 23.0 39.0 205-209 35.007695390781564 38.0 37.0 39.0 24.0 39.0 210-214 34.69748640137418 38.0 36.8 39.0 17.2 39.0 215-219 34.29559690810193 38.0 36.2 38.2 15.0 39.0 220-224 34.07002576581735 37.4 35.8 38.0 15.0 39.0 225-229 33.51614085313484 37.0 33.8 38.0 15.0 39.0 230-234 33.457028342399084 37.0 34.0 38.0 15.0 38.2 235-239 33.137497852848554 37.0 33.2 38.0 15.0 38.0 240-244 32.33777268823361 37.0 31.4 38.0 14.0 38.0 245-249 32.20059547666763 37.0 31.6 37.4 14.0 38.0 250-251 30.124592041225306 35.0 23.0 37.0 14.0 37.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3007758052773326 1101 2 0.2208306018290287 1101 3 0.35413031612108625 1101 4 0.23429085577409836 1101 5 0.20833917402786284 1101 6 0.31111930484976114 1101 7 0.09443946093202982 1101 8 0.7511310412831307 1101 9 0.13442239562822067 1101 10-14 0.18966243210842038 1101 15-19 0.12494132100550814 1101 20-24 0.2167824944180623 1101 25-29 0.3813820348428436 1101 30-34 0.4446083043092699 1101 35-39 0.021490828257270778 1101 40-44 -0.07736115329797855 1101 45-49 0.2509759142681176 1101 50-54 0.35650685079964006 1101 55-59 0.3273168671487454 1101 60-64 0.22752249837930094 1101 65-69 0.04571946369388513 1101 70-74 0.23688388147161277 1101 75-79 0.25601040734345304 1101 80-84 0.08454307540814199 1101 85-89 0.3083214499111193 1101 90-94 0.32221671707096533 1101 95-99 0.30646876420159685 1101 100-104 0.3897265913070953 1101 105-109 0.2214781508074921 1101 110-114 0.48541989664872887 1101 115-119 0.45181167247943677 1101 120-124 0.7265887260150166 1101 125-129 0.2675997525079197 1101 130-134 0.3349379331862252 1101 135-139 0.5581392868190846 1101 140-144 0.3033980273770922 1101 145-149 0.10737583698892905 1101 150-154 0.03838589777976864 1101 155-159 0.5037847209768103 1101 160-164 0.07175052622026357 1101 165-169 -0.0796558462290804 1101 170-174 -0.3112715516016493 1101 175-179 -0.39359066009867405 1101 180-184 -0.1113133377341029 1101 185-189 -0.16329839136323443 1101 190-194 -0.30570533938773536 1101 195-199 0.2993771955265174 1101 200-204 0.5364634598708307 1101 205-209 0.27462678440140564 1101 210-214 0.5984463978910028 1101 215-219 0.4878507721455776 1101 220-224 0.20019762773918615 1101 225-229 -0.5393628553765453 1101 230-234 -0.6013851398665224 1101 235-239 -0.8045238978838469 1101 240-244 -0.3176773898378791 1101 245-249 -0.3196345188154268 1101 250-251 -0.5064659874911968 1102 1 -0.3887403237549236 1102 2 0.23242334376450913 1102 3 0.21199321934689408 1102 4 0.23529892029022648 1102 5 -0.29770921306891296 1102 6 0.21636124033363302 1102 7 0.5087539770610618 1102 8 0.3423608799928104 1102 9 0.20649900853144487 1102 10-14 0.26688017404390507 1102 15-19 0.1686913210055181 1102 20-24 0.10881878474063456 1102 25-29 -0.3236582877377998 1102 30-34 0.16900346559958734 1102 35-39 0.4357045379346971 1102 40-44 0.018909007992348847 1102 45-49 -0.008701505086712302 1102 50-54 0.402877818541576 1102 55-59 -0.06391297156093145 1102 60-64 -0.2581629854916656 1102 65-69 -0.17373618146741165 1102 70-74 0.3402104943748441 1102 75-79 -0.2573968507210651 1102 80-84 0.19462372056943167 1102 85-89 0.3756601595885485 1102 90-94 -0.29471876680000264 1102 95-99 -0.2305272035403334 1102 100-104 -0.12186615062839223 1102 105-109 -0.02771539757959829 1102 110-114 -0.08333010335127256 1102 115-119 0.27731570473749656 1102 120-124 0.5934234034343717 1102 125-129 0.4541924944434115 1102 130-134 0.17314357834751348 1102 135-139 0.014590899722307427 1102 140-144 0.5397891564093555 1102 145-149 0.3023355144082842 1102 150-154 -0.04608990867184559 1102 155-159 -0.2310943112812538 1102 160-164 -0.36312850603780333 1102 165-169 0.2821385086096271 1102 170-174 0.6392324806564105 1102 175-179 0.636046436675521 1102 180-184 0.8974568235562188 1102 185-189 0.8588790279916054 1102 190-194 0.7390527251283885 1102 195-199 0.74363122778459 1102 200-204 0.7507779759998598 1102 205-209 0.6989211392401131 1102 210-214 1.2825189785361673 1102 215-219 1.2327096431133242 1102 220-224 1.9327581116101555 1102 225-229 2.229387144623459 1102 230-234 1.304159214972188 1102 235-239 1.5291454569548648 1102 240-244 2.7863548682266384 1102 245-249 2.0811719327974743 1102 250-251 1.6210541737991235 1103 1 -0.014700385198857191 1103 2 0.08367782405125013 1103 3 0.28418983993061175 1103 4 0.3062154589486994 1103 5 0.4355217137104006 1103 6 0.20893676516722337 1103 7 0.2744989847415553 1103 8 -0.19604157776448972 1103 9 0.30480930039012577 1103 10-14 0.1959124321084218 1103 15-19 -0.0900388377246486 1103 20-24 0.12238765314822331 1103 25-29 0.278901876112684 1103 30-34 0.3581995741505466 1103 35-39 -0.1334198860284488 1103 40-44 0.5557241641623349 1103 45-49 0.36922988252209166 1103 50-54 0.4047211365139347 1103 55-59 0.23163234333922134 1103 60-64 0.3774232920300946 1103 65-69 0.27582859067801024 1103 70-74 0.6591557068684395 1103 75-79 0.1414766771847198 1103 80-84 0.12442402778909667 1103 85-89 0.41764684673651686 1103 90-94 0.4662643361185843 1103 95-99 0.5723913832492187 1103 100-104 0.6760857182912332 1103 105-109 -0.06622026189091912 1103 110-114 0.5833365633153988 1103 115-119 -0.2372260259332606 1103 120-124 0.7436026149038995 1103 125-129 0.6308438001269678 1103 130-134 0.47754706017035176 1103 135-139 0.17480595348575179 1103 140-144 0.8467015988056659 1103 145-149 0.37791155127464293 1103 150-154 0.6253898660337356 1103 155-159 0.5675744035164954 1103 160-164 0.6577624309821672 1103 165-169 0.4983699474217147 1103 170-174 0.9342145595094564 1103 175-179 0.5717863240283165 1103 180-184 0.8342719797262106 1103 185-189 1.115868275303427 1103 190-194 0.4956835495011518 1103 195-199 0.7116291796535066 1103 200-204 0.7133483805057494 1103 205-209 0.8595970224966507 1103 210-214 0.7128313185259358 1103 215-219 0.06706704198685287 1103 220-224 0.46701310392966633 1103 225-229 0.7135141287504467 1103 230-234 0.9283271617207802 1103 235-239 0.34537689576694675 1103 240-244 0.5811321339716429 1103 245-249 0.7521908780099693 1103 250-251 1.4967582188580124 2112 1 0.39116042066194723 2112 2 -0.12315978278635242 2112 3 0.052688008428781075 2112 4 0.026117778851023843 2112 5 0.49583917402786426 2112 6 -0.5283037720733148 2112 7 -0.49546438522181546 2112 8 0.007621425898513223 2112 9 4.739562822209109E-5 2112 10-14 0.16822012441610923 2112 15-19 0.03484516715935371 2112 20-24 0.1575036482642176 2112 25-29 0.2007089579197725 2112 30-34 -0.1670263110753467 2112 35-39 -0.5268264794350443 2112 40-44 -0.07865923022105648 2112 45-49 -0.2944087011164953 2112 50-54 -0.034839303046510395 2112 55-59 -0.26301967131279014 2112 60-64 0.048387882994681775 2112 65-69 -0.11577092092150565 2112 70-74 -0.5693180416053067 2112 75-79 -1.171441515733477 2112 80-84 -1.2094953861303281 2112 85-89 -1.0653323962427237 2112 90-94 -1.0143217444675017 2112 95-99 -1.3605023896445587 2112 100-104 -1.518590716385205 2112 105-109 -1.2689064645771282 2112 110-114 -1.3817916418128107 2112 115-119 -1.3726594813667177 2112 120-124 -1.2037478124465224 2112 125-129 -0.9860060167228539 2112 130-134 -1.55712937450609 2112 135-139 -0.482629943950144 2112 140-144 -1.309342357238286 2112 145-149 -1.087143393780302 2112 150-154 -0.9151717945279287 2112 155-159 -0.39804220210010754 2112 160-164 -0.7762302430104953 2112 165-169 -1.0029731539213884 2112 170-174 -0.7861273208324135 2112 175-179 -0.963109890867905 2112 180-184 -0.3447267992725713 2112 185-189 -0.9107983913632367 2112 190-194 -0.4916668778492763 2112 195-199 -0.26019011216578747 2112 200-204 -0.6319019247445539 2112 205-209 -0.5169597540601245 2112 210-214 -0.33395744826283646 2112 215-219 -0.3104665355467304 2112 220-224 -0.4645619876454319 2112 225-229 0.012464067700378223 2112 230-234 -0.43479860140498516 2112 235-239 -0.46058159019153777 2112 240-244 0.37732261016211766 2112 245-249 -0.34617298035389155 2112 250-251 0.31348593558572446 1113 1 0.017881068435229963 1113 2 0.3000520930570971 1113 3 0.216520666998278 1113 4 0.1591812066512901 1113 5 0.22917250736119854 1113 6 0.02932105923572692 1113 7 0.18928595216009825 1113 8 0.1598481465462882 1113 9 0.38250353597909736 1113 10-14 0.15029839702069125 1113 15-19 0.2090421981984889 1113 20-24 0.047868020733851324 1113 25-29 -0.060613579192249745 1113 30-34 -0.2484838009538919 1113 35-39 0.18788337211691442 1113 40-44 0.010193671263422743 1113 45-49 0.29571275637338346 1113 50-54 0.3681296578171853 1113 55-59 -0.07964585214950404 1113 60-64 -0.25482399284877033 1113 65-69 0.16638832334300702 1113 70-74 -0.008346381686280324 1113 75-79 -0.647662838270584 1113 80-84 -0.3718165737146677 1113 85-89 -0.29847679570291064 1113 90-94 0.05423426093061323 1113 95-99 -0.39753342878086073 1113 100-104 -0.1316001630788648 1113 105-109 -0.2886095684907559 1113 110-114 -0.38157571738636165 1113 115-119 -0.07565543278372644 1113 120-124 0.06874881373431663 1113 125-129 -0.4974770018780461 1113 130-134 0.12951030160727584 1113 135-139 0.42743753243312455 1113 140-144 0.3559747817630523 1113 145-149 -0.5960232858180845 1113 150-154 -0.12181147064128339 1113 155-159 -0.08689510358459529 1113 160-164 -0.2855959650078077 1113 165-169 -0.46721067079048595 1113 170-174 -0.47514216563673983 1113 175-179 -0.1983055723793754 1113 180-184 -0.020030442997260423 1113 185-189 -0.2525527773281411 1113 190-194 0.06293501148945779 1113 195-199 0.29943202008792014 1113 200-204 -0.028339171708118727 1113 205-209 -0.33288418051087376 1113 210-214 -0.021838689828292956 1113 215-219 -1.0308444032930169 1113 220-224 -0.47584403892748384 1113 225-229 -1.0214900483589986 1113 230-234 -0.604839087234943 1113 235-239 -0.5780984592873537 1113 240-244 0.46434015402176954 1113 245-249 -0.22709065916630422 1113 250-251 0.009707258122837459 2113 1 0.45269888220040855 2113 2 0.4614556018290301 2113 3 0.3449957007364688 2113 4 0.3030408557740998 2113 5 0.5573776355663256 2113 6 0.44092699715745454 2113 7 0.4737663840089539 2113 8 0.6230060412831335 2113 9 0.5692781648589929 2113 10-14 0.4912970474930347 2113 15-19 0.188691321005507 2113 20-24 0.17596518672576167 2113 25-29 0.25301665022745823 2113 30-34 0.03605061200157422 2113 35-39 -0.06528801789657734 2113 40-44 0.20749461593279506 2113 45-49 0.02251437580657978 2113 50-54 -0.04714699535420408 2113 55-59 -0.49071197900509844 2113 60-64 -0.019304424697622835 2113 65-69 -0.20807861322919052 2113 70-74 0.258374266087003 2113 75-79 0.29009694580499 2113 80-84 0.33819692156199466 2113 85-89 0.6300522191418878 2113 90-94 0.551832101686351 2113 95-99 0.3994976103554464 2113 100-104 0.24756312976863626 2113 105-109 0.4234012277305723 2113 110-114 0.39051605049488813 2113 115-119 0.4242635955563614 2113 120-124 1.119329110630403 2113 125-129 0.3032247525079299 2113 130-134 0.17517831780160975 2113 135-139 -0.10416840548860762 2113 140-144 0.4045037966078695 2113 145-149 -0.0840664707033767 2113 150-154 -0.016710256066389206 2113 155-159 0.5773424132845051 2113 160-164 0.29146206468180225 2113 165-169 -0.11681930776754257 2113 170-174 0.2661803714752722 2113 175-179 0.31996703220901423 2113 180-184 0.13527320072743265 2113 185-189 0.396893916329077 2113 190-194 0.1083331221507251 2113 195-199 0.4567329647572862 2113 200-204 0.5157903829477632 2113 205-209 0.10457870747833908 2113 210-214 -0.1462651405705344 2113 215-219 -0.5535434586236505 2113 220-224 -0.27071583379927233 2113 225-229 -0.6890743938380837 2113 230-234 -0.4224909090972986 2113 235-239 0.15172610211615734 2113 240-244 0.38347645631596805 2113 245-249 0.17690394272303678 2113 250-251 -0.4865140644142727 1112 1 -0.6088395793380528 1112 2 -0.33854439817097415 1112 3 -0.2703889146481444 1112 4 0.0568870096202545 1112 5 -0.8887762105875225 1112 6 0.36400392023437433 1112 7 -0.080079769837198 1112 8 -0.10007088179379053 1112 9 -0.10764491206408877 1112 10-14 -0.45639526019927246 1112 15-19 0.2009990133132078 1112 20-24 -0.28557327481270534 1112 25-29 -0.14698334977254035 1112 30-34 0.11912753507850482 1112 35-39 0.023942751334189438 1112 40-44 0.007494615932792215 1112 45-49 0.05328360657581044 1112 50-54 -0.2717623799695872 1112 55-59 0.10621109791798489 1112 60-64 0.03608019068698809 1112 65-69 0.20730600215542694 1112 70-74 -0.027779580066848553 1112 75-79 0.05317386888191322 1112 80-84 0.04588922925429273 1112 85-89 0.05159068068035566 1112 90-94 -0.05124482139057562 1112 95-99 -0.10819469733686304 1112 100-104 -0.090898408692901 1112 105-109 0.18340122773057033 1112 110-114 0.15666989664872233 1112 115-119 0.01195590324866913 1112 120-124 0.6239444952457802 1112 125-129 0.06630167558484601 1112 130-134 0.08287062549392488 1112 135-139 -0.03032225164245972 1112 140-144 0.2475807196847839 1112 145-149 0.25747199083508576 1112 150-154 0.10636666701053343 1112 155-159 0.3434962594383464 1112 160-164 -0.39776870454896596 1112 165-169 -0.6460500769983142 1112 170-174 0.07541114070604493 1112 175-179 -0.16618681394482593 1112 180-184 0.3229655084197418 1112 185-189 -0.7077214682863087 1112 190-194 -0.33166687784927973 1112 195-199 -0.34326703524271096 1112 200-204 -0.5149788478214745 1112 205-209 -0.48926744636782615 1112 210-214 -0.4447266790320654 1112 215-219 0.12337961829942401 1112 220-224 -0.11071583379927574 1112 225-229 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2110 105-109 -0.456747655644115 2110 110-114 -0.09945913560933661 2110 115-119 -0.7355875210689504 2110 120-124 -3.625931435275305 2110 125-129 -1.0103236345888504 2110 130-134 -0.49137255068474417 2110 135-139 -0.346699422858336 2110 140-144 -0.6924689081067825 2110 145-149 -0.6557290017207436 2110 150-154 -0.25576732802668545 2110 155-159 -0.3343201177328652 2110 160-164 -0.1050639899087642 2110 165-169 0.10471915377091534 2110 170-174 -0.7478642935371411 2110 175-179 -0.8704051762277061 2110 180-184 -0.28963995063732995 2110 185-189 -0.5088629074922721 2110 190-194 -0.8286892103554777 2110 195-199 -0.3015300625379922 2110 200-204 -0.7008349272259409 2110 205-209 -1.2268853123727865 2110 210-214 -0.3271584408186641 2110 215-219 -0.44470971172538043 2110 220-224 -0.3124031787124224 2110 225-229 0.20358069301055082 2110 230-234 0.9202882472302534 2110 235-239 0.3968873924387424 2110 240-244 -0.32654835757981715 2110 245-249 -0.23495709946058696 2110 250-251 -0.5402361487815206 2109 1 0.15882665273495888 2109 2 0.09874373742224662 2109 3 -0.03622985337043616 2109 4 -0.042721856090309984 2109 5 -0.6634828598704416 2109 6 0.3079413387480656 2109 7 -0.06286986110186632 2109 8 0.18571790568991275 2109 9 -0.1592746382700838 2109 10-14 0.1586242965151996 2109 15-19 -0.08385105187585395 2109 20-24 -0.22617301405651347 2109 25-29 -0.08779169397071485 2109 30-34 -0.14892983128395088 2109 35-39 0.007984472325063052 2109 40-44 0.04504350771896526 2109 45-49 -0.1820749331895044 2109 50-54 0.027481427070824793 2109 55-59 0.17917068070806152 2109 60-64 -0.5280919084003628 2109 65-69 -0.07488434986544235 2109 70-74 0.14562328825127224 2109 75-79 -0.5317544231650189 2109 80-84 0.0839074821878043 2109 85-89 0.09200789058908754 2109 90-94 0.13874214079977065 2109 95-99 0.07537766250668199 2109 100-104 -0.31530518835391774 2109 105-109 -0.14843710342979222 2109 110-114 -0.33417756097838947 2109 115-119 0.2611866724794325 2109 120-124 0.25810355652348704 2109 125-129 0.2476315321689455 2109 130-134 -0.503674354949375 2109 135-139 -0.2647420691131259 2109 140-144 -0.11455748109748498 2109 145-149 0.1382021081753706 2109 150-154 0.06391555879671529 2109 155-159 -0.3115754485147164 2109 160-164 -0.0029316771695633292 2109 165-169 -0.04443338860196633 2109 170-174 -0.3023202804152163 2109 175-179 -0.40895082959019646 2109 180-184 -0.018781558073094118 2109 185-189 0.08648974422998634 2109 190-194 0.37967601654447236 2109 195-199 0.03597677179770642 2109 200-204 -0.027625523180013545 2109 205-209 0.10624754711327 2109 210-214 -1.4213629241428976 2109 215-219 -0.9112488041255986 2109 220-224 0.24505991587478348 2109 225-229 0.4276922293692209 2109 230-234 0.030129690641956586 2109 235-239 -0.24149423686689886 2109 240-244 0.3251192203316151 2109 245-249 -0.04463451881542824 2109 250-251 0.4853242667460904 2108 1 0.4773631068646367 2108 2 0.30590004627347156 2108 3 0.10958666532743422 2108 4 0.29034244307568713 2108 5 0.11806139625008427 2108 6 0.5740161302465836 2108 7 0.4332291434717135 2108 8 -0.03731141903433155 2108 9 0.0032219988028217017 2108 10-14 -0.05488121868523166 2108 15-19 0.04011989243408465 2108 20-24 -0.20777107701050568 2108 25-29 0.11382251103332663 2108 30-34 0.27565989161085724 2108 35-39 -0.45405480666337183 2108 40-44 -0.09189488345671037 2108 45-49 0.289864803157009 2108 50-54 0.13170526349805556 2108 55-59 0.19353710524398338 2108 60-64 -0.15908464447784354 2108 65-69 0.34884446369388655 2108 70-74 0.19248904020177093 2108 75-79 0.06846080416884348 2108 80-84 -0.06605216268710024 2108 85-89 -0.9188610897714184 2108 90-94 0.08848655834080432 2108 95-99 -0.10379909294126577 2108 100-104 0.025292067497574067 2108 105-109 -0.10431549998615708 2108 110-114 0.3388921188709446 2108 115-119 0.29928191057467046 2108 120-124 0.7880470593483508 2108 125-129 0.1927485620317313 2108 130-134 0.33151531413859914 2108 135-139 -0.028368649688857772 2108 140-144 0.24352699563106484 2108 145-149 0.49854647190957024 2108 150-154 0.7460247866686558 2108 155-159 0.25328868923078574 2108 160-164 0.22919100241074375 2108 165-169 -0.260360211308452 2108 170-174 0.49294471823962027 2108 175-179 0.4701990224410153 2108 180-184 0.4850656305198626 2108 185-189 0.2523762118113666 2108 190-194 0.016318470136070573 2108 195-199 0.6100418780661911 2108 200-204 0.01811028526766023 2108 205-209 0.20245416535378524 2108 210-214 -0.08716868147407553 2108 215-219 -0.31388533896552673 2108 220-224 -0.9901297532131963 2108 225-229 0.2531966684329774 2108 230-234 0.007692241085862861 2108 235-239 1.4628372132272602 2108 240-244 0.7938305466700584 2108 245-249 0.9426670684861591 2108 250-251 1.0840598061595976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 7.0 21 29.0 22 138.0 23 265.0 24 232.0 25 108.0 26 92.0 27 100.0 28 143.0 29 200.0 30 266.0 31 324.0 32 440.0 33 546.0 34 675.0 35 953.0 36 1532.0 37 4508.0 38 6904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.71714858288005 29.61351273976525 9.630689951331235 25.038648726023478 2 41.20240480961924 25.01002004008016 21.80933295161752 11.97824219868308 3 38.74033781849413 20.389350128829086 24.906956770684225 15.963355281992555 4 39.24420269109648 20.30346407099914 19.788147724019467 20.664185513884913 5 33.07185800171772 20.48668766103636 31.22244488977956 15.21900944746636 6 31.983967935871743 20.143143429716577 31.05639851130833 16.81649012310335 7 17.51503006012024 47.827082736902376 19.856856570283423 14.80103063269396 8 27.346120813054682 23.492699685084453 31.74921271113656 17.411966790724307 9 20.80732894360149 20.080160320641284 30.98196392785571 28.130546807901517 10-14 25.584884053821927 34.3704551961065 22.055539650730033 17.98912109934154 15-19 23.216719152590894 32.6286859433152 21.789865445176066 22.364729458917836 20-24 24.9481820784426 30.430002862868594 25.560835957629546 19.06097910105926 25-29 19.233896364156884 28.490123103349557 32.34239908388205 19.93358144861151 30-34 19.59576295448039 28.666475808760378 29.630689951331235 22.107071285428 35-39 22.39793873461208 28.929859719438877 28.30231892356141 20.36988262238763 40-44 19.15602633839107 29.92270254795305 26.511308330947607 24.409962782708273 45-49 21.53564271399943 28.18894932722588 27.225880332092757 23.049527626681936 50-54 23.519038076152306 28.690523904952762 25.08559977097051 22.70483824792442 55-59 21.366160893215003 25.2046951044947 30.315488119095335 23.113655883194962 60-64 22.310907529344405 25.80475236186659 29.41654738047524 22.46779272831377 65-69 20.203836243916403 30.021185227598053 26.92356140853135 22.851417119954196 70-74 20.506155167477814 28.377898654451762 29.043229315774404 22.072716862296023 75-79 21.078728886344116 27.309476095047238 28.319496135127398 23.292298883481248 80-84 22.281133695963355 28.770684225594046 25.501288290867446 23.446893787575153 85-89 21.602061265387917 26.57658173489837 26.75293444030919 25.068422559404524 90-94 23.03006012024048 28.968794732321783 27.07586601774978 20.925279129687947 95-99 23.44231319782422 26.99456054967077 28.600057257371887 20.963068995133124 100-104 22.446034926996848 27.23275121671915 26.542227311766393 23.778986544517608 105-109 24.778700257658173 28.119095333524193 25.194388777555112 21.907815631262526 110-114 22.276553106212425 26.460921843687373 30.064700830231892 21.19782421986831 115-119 24.06183796163756 26.568565702834242 27.8946464357286 21.4749498997996 120-124 20.586315488119094 27.372459204122528 25.96736329802462 26.073862009733755 125-129 19.832808474091042 25.67764099627827 29.012310334955625 25.477240194675066 130-134 22.961351273976526 28.101918121958203 27.77784139707987 21.1588892069854 135-139 23.404523332379043 25.558545662754078 25.497852848554253 25.539078156312627 140-144 21.788720297738333 26.82736902376181 25.649012310334957 25.7348983681649 145-149 22.50672774119668 26.517034068136276 28.03435442313198 22.94188376753507 150-154 21.269968508445462 29.22874320068709 24.90466647580876 24.59662181505869 155-159 21.084454623532782 26.482679645004293 29.952476381334098 22.480389350128828 160-164 21.96278270827369 31.295734325794445 25.125679931291156 21.61580303464071 165-169 21.880332092756944 28.515316346979674 26.418551388491267 23.185800171772115 170-174 21.103922129974233 30.304036644718007 25.45891783567134 23.133123389636413 175-179 19.210993415402232 28.367592327512163 27.409103922129972 25.01231033495562 180-184 21.389063841969655 28.075579730890354 27.421700543945033 23.113655883194962 185-189 20.36415688519897 27.128542799885487 27.906097910105927 24.60120240480962 190-194 19.607214428857716 27.925565416547382 28.04695104494704 24.420269109647865 195-199 19.387346120813053 31.5785857429144 26.052104208416832 22.981963927855713 200-204 20.700830231892358 27.93930718580017 25.991411394217007 25.368451188090468 205-209 20.267964500429432 27.044947036931006 28.72602347552247 23.96106498711709 210-214 23.120526767821357 26.254795304895506 28.861150873174925 21.763527054108216 215-219 20.65387918694532 29.835671342685373 27.212138562839964 22.298310907529345 220-224 19.484683653020326 28.756942456341257 27.034640709991407 24.72373318064701 225-229 22.784998568565705 28.38820498139135 26.84340108789007 21.983395362152876 230-234 21.287145720011452 28.932150014314345 27.229315774405954 22.551388491268252 235-239 20.524477526481537 28.256513026052104 26.941883767535067 24.27712567993129 240-244 21.433724592041226 31.991983967935873 25.07643859146865 21.497852848554253 245-249 22.022330375035786 29.32264529058116 25.6856570283424 22.969367306040652 250-251 20.538219295734326 27.4205553965073 24.116805038648724 27.924420269109646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.3333333333333333 20 0.3333333333333333 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 1.0 28 3.3333333333333335 29 4.999999999999999 30 8.33333333333333 31 5.999999999999998 32 8.33333333333333 33 17.66666666666667 34 41.33333333333335 35 209.3333333333349 36 215.33333333333508 37 79.66666666666661 38 138.66666666666623 39 236.33333333333567 40 396.9999999999949 41 376.6666666666627 42 587.9999999999969 43 706.6666666666771 44 791.6666666666868 45 794.0000000000203 46 798.3333333333542 47 692.3333333333421 48 715.6666666666781 49 856.6666666666941 50 640.0000000000028 51 252.66666666666947 52 64.00000000000017 53 22.666666666666654 54 17.000000000000007 55 13.33333333333334 56 12.333333333333337 57 6.333333333333331 58 4.666666666666666 59 5.666666666666665 60 3.666666666666667 61 3.0 62 3.0 63 1.0 64 0.6666666666666666 65 0.3333333333333333 66 0.0 67 0.6666666666666666 68 0.3333333333333333 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-251 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 17465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.667384260084276 #Duplication Level Percentage of deduplicated Percentage of total 1 69.52132975905161 33.83421269655797 2 17.819959198302698 17.345016036056414 3 6.6688923651676015 9.736726419742618 4 2.7651594919090137 5.382923181326215 5 1.182836014117097 2.8782767407851617 6 0.7345625456894201 2.1449542604479634 7 0.4482040311749795 1.5269042468478065 8 0.22410175238645347 0.8725156877399833 9 0.1120508570830208 0.49078999064980433 >10 0.28635218257121603 2.211584464361257 >50 0.04980037948356132 1.5147838778903102 >100 0.16185123332157428 15.359908521807744 >500 0.02490018974178066 6.701403875786732 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 582 3.3323790438018897 TruSeq Adapter, Index 7 (97% over 38bp) CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 524 3.000286286859433 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 477 2.7311766389922703 No Hit CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC 413 2.364729458917836 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC 267 1.528771829373032 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 247 1.414257085599771 No Hit GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 191 1.0936158030346408 No Hit GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC 143 0.8187804179788147 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA 131 0.7500715717148583 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG 131 0.7500715717148583 No Hit GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 114 0.6527340395075866 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT 113 0.6470083023189236 No Hit GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC 105 0.6012024048096193 No Hit CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG 102 0.5840251932436301 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA 101 0.578299456054967 No Hit GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA 73 0.4179788147724019 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 61 0.34926996850844544 No Hit GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA 60 0.34354423131978246 No Hit GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 56 0.32064128256513025 No Hit GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA 46 0.26338391067849987 No Hit GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA 40 0.22902948754652158 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 31 0.17749785284855424 No Hit GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 22 0.1259662181505869 No Hit GGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTC 18 0.10306326939593473 No Hit GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT 18 0.10306326939593473 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-14 0.010306326939593474 0.0 0.0 15-19 0.01832235900372173 0.0 0.0 20-24 0.022902948754652163 0.0 0.0 25-29 0.022902948754652163 0.0 0.0 30-34 0.0286286859433152 0.0 0.0 35-39 0.03320927569424563 0.0 0.0 40-44 0.04008016032064128 0.0 0.0 45-49 0.056112224448897796 0.0 0.0 50-54 0.07328943601488691 0.0 0.0 55-59 0.0858860578299456 0.0 0.0 60-64 0.12024048096192383 0.0 0.0 65-69 0.17978814772401946 0.0 0.0 70-74 0.27139994274262813 0.0 0.0 75-79 0.3641568851989693 0.0 0.0 80-84 0.45118809046664765 0.0 0.0 85-89 0.6206699112510736 0.0 0.0 90-94 0.9161179501860864 0.0 0.0 95-99 1.1371314056684798 0.0 0.0 100-104 1.3753220727168622 0.0 0.0 105-109 1.7142857142857142 0.0 0.0 110-114 2.183796163756084 0.0 0.0 115-119 2.643000286286859 0.0 0.0 120-124 3.1342685370741483 0.0 0.0 125-129 3.6862296020612653 0.0 0.0 130-134 4.232464929859719 0.0 0.0 135-139 4.8622960206126535 0.0 0.0 140-144 5.489836816490123 0.0 0.0 145-149 6.152877182937303 0.0 0.0 150-154 6.954480389350129 0.0 0.0 155-159 7.721729172630977 0.0 0.0 160-164 8.608073289436016 0.0 0.0 165-169 9.422273117663899 0.0 0.0 170-174 10.52963068995133 0.0 0.0 175-179 11.507586601774978 0.0 0.0 180-184 12.444317205840253 0.0 0.0 185-189 13.468079015173203 0.0 0.0 190-194 14.463212138562842 0.0 0.0 195-199 15.592327512167191 0.0 0.0 200-204 16.679072430575435 0.0 0.0 205-209 17.724592041225307 0.0 0.0 210-214 18.778127683939307 0.0 0.0 215-219 19.785857429144006 0.0 0.0 220-224 20.833667334669336 0.0 0.0 225-229 21.7875751503006 0.0 0.0 230-234 22.751789292871457 0.0 0.0 235-239 23.819066704838246 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGAGG 40 0.0 245.00002 245 GGGTTAT 220 0.0 245.0 2 ATTAGTA 220 0.0 245.0 7 CGGGTTA 220 0.0 245.0 1 GGGGGGA 225 0.0 245.0 1 TATTAGT 220 0.0 245.0 6 GGTTATT 230 0.0 234.34782 3 TTATTAG 235 0.0 229.36171 5 GTTATTA 235 0.0 229.36171 4 TAGTAGA 240 0.0 224.58333 9 GAGCGAA 170 0.0 223.38235 6 GCGAAAG 170 0.0 223.38235 8 CGAAAGC 170 0.0 223.38235 9 AGCGAAA 170 0.0 223.38235 7 GGAGCGA 170 0.0 223.38235 5 GGGGGAG 250 0.0 220.5 2 GGGGAGC 275 0.0 200.45453 3 TTAGTAG 275 0.0 195.99998 8 GGGAGCG 200 0.0 189.875 4 CGAGGAC 75 0.0 163.33333 245 >>END_MODULE