FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated h2.341000000084e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated h2.341000000084e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548653
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT18939034.51908583385127TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG30160.5497099259459075No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC25740.4691489885228004No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT20460.3729132985694054TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGACAAGTGCAGTCAGGACAATGAGATAAA19820.3612483664538424No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA19110.3483075823881397No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA13050.23785525641890232No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC12550.2287420282036187No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT12100.22054012280986343No Hit
GGGGGGAGCGAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG11720.21361406936624788No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11470.20905745525860606No Hit
CGGGTTATTAGTAGAAACAAGGAGACACTAGGGTATTTCTCCTTTTGCCT11330.20650575135832666No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA10650.19411176098554095No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG9900.18044191866261552No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATTTCGTAT9650.17588530455497373TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGGAGAAGAAGCTCTCACCACTATTCTAGCTCTTA9600.17497398173344536No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA7490.1365161586649485No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC7400.13487577758619745No Hit
CGGGTTATTAGTAGAAACAAGGGGGCTGCACAGTCCCTGGAGAGCCTGTG7020.12794972414258193No Hit
GGGGGGAGCAAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG6920.1261270784995252No Hit
CGGGTTATTAGTAGAAACAAGGACAGAAAGGTCAGCAGTTCTTGAGAACA6660.12138819982757772No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG6600.12029461244174369No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGTATTTGTACTGTGACTGGCTCCC6590.120112347877438No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT6500.11847196679868696No Hit
GGGGGGAGCGAAAGCAGGAAGCCAGTACCAGGGCTAAGCTGGAAAGGCAA6350.1157379983341019No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGCAT6310.1150089400768792TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCC6220.11336855899812814TruSeq Adapter, Index 6 (96% over 33bp)
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT6000.10935873858340336No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG5940.10826515119756933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA26750.0239.958511
GGGTTAT28100.0228.430272
GGGGGGA80100.0218.691641
TATTAGT30150.0211.679646
TTATTAG30200.0211.329185
GTTATTA30300.0211.0364
TAGTAGA31100.0204.425759
GGAGCGA55000.0202.901065
GGGAGCG55550.0201.112674
ATTAGTA31700.0200.942927
GAGCGAA55950.0199.455936
GGTTATT32200.0199.344423
GCGAAAG55650.0199.210438
AGCGAAA56900.0195.264667
CGAAAGC56850.0194.789989
GGGGGAG90200.0193.253342
GGGGAGC91750.0188.386433
TTAGTAG33950.0187.264838
GGAGCAA34700.0176.156945
GAGCAAA35500.0170.116826
Produced by FastQC (version 0.11.2)