FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated h1.34100000008460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated h1.34100000008460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318859
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT11050234.6554433150703TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT64182.012801896763146TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC11800.3700695291649287No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATTTCGTAT11690.36661972846932345TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC8370.26249847111105534No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC7320.2295685553802778No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT6760.21200593365719644No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA6710.21043784243192132No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA6300.19757949438466532No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG5860.18378029160224424No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG5250.16464957865388777No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG4540.14238268325498105No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC4500.14112821027476094No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA4040.12670177100222982No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT3230.10129869315277287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA12900.0239.302351
GGGTTAT13750.0224.509082
GTTATTA14900.0206.359074
TATTAGT15200.0203.09216
GGGGGGA34700.0199.459661
ATTAGTA15550.0198.520897
TAGTAGA15500.0198.370979
TTATTAG15650.0196.469655
TTAGTAG15900.0194.150968
GGTTATT16000.0192.93753
GGGAGCG23050.0189.728874
GGAGCGA23250.0188.096775
GAGCGAA23550.0186.220816
GCGAAAG23850.0180.796658
AGCGAAA24000.0180.177087
GGGGAGC39000.0177.467943
CGAAAGC24550.0175.641569
GGGGGAG39650.0174.249682
GGAGCAA15550.0162.282945
GAGCAAA17200.0145.29076
Produced by FastQC (version 0.11.2)