FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated g7.34100000008751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated g7.34100000008751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT45.405405405405405TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAAGCAATCTCGTAT34.054054054054054TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTCGTATCTCGTAT22.7027027027027026TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGCAATCTCGTAT22.7027027027027026TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGGTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACATTCAGAAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 27 (97% over 37bp)
ACAGAGATTCGCTTGGAGAAACTGTGATGGGAATGGGAGACCTTCACTAC11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTAACTAAATCTCCACGATGAC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGAGATTCCATCTCGTAT11.3513513513513513TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAATTACTCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 27 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGCCCAAAACAGACATATCAGTTAAGACTC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCGAATTCGTATCTCGTATG11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAATCCGGAGAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 3 (97% over 34bp)
ATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTAT11.3513513513513513No Hit
CCCCACTGATGCGCAACATGAAAATCACATGATGAAGTTTCTAAAAACAA11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTACATTCAGAAATCTCGTATG11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGAGCTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 6 (97% over 36bp)
TACCTCCAGGGCAATCTCTGAACAGGTAATAAGACTAACTTCTCTCCCTC11.3513513513513513No Hit
TAGAATCTCCCGATTCCACCAATCATTCTTCCGACAGATGCTCTGATTTC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGCTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAATAACTCCGGAGAATCTCGTAT11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCCAACTTCTGTACTCCTA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCGTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 13 (97% over 38bp)
AATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCA11.3513513513513513No Hit
ATTGCATGGCTTCCTTAAAATCATTAATGTGTAGAAATTCGTCAATAATA11.3513513513513513No Hit
CGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTGGAATTTCTA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
GGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTCTC11.3513513513513513No Hit
TCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCG11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA11.3513513513513513No Hit
CCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCAGTCTCTGCGCG11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCAGAAATCTCGTATGC11.3513513513513513TruSeq Adapter, Index 3 (97% over 34bp)
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCAGCTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 7 (97% over 36bp)
GGAGCAAAAGCAGGCAGAGAGGCAGGCAGAGAGAGAGAGAGGAGGGAAGC11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGAAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
ATACTGATATAAACAAATAAAAAACAAACAAAAGGGAATCCTCTTCCTTA11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGTCGCCTTATACTCTAGCTCTCCGTCCAA11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA11.3513513513513513No Hit
CTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTG11.3513513513513513No Hit
ATCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCA11.3513513513513513No Hit
GATCGGATGAGCACACGTCTGAACTCCAGTCAAATTACTCGATCTCGTAT11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GATCGGAAGAGCAAACGTCTGAACTCCAGTCACGAGATCGTATCTCGTAT11.3513513513513513TruSeq Adapter, Index 9 (97% over 35bp)
ACTGTCGCCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTT11.3513513513513513No Hit
ACAATCTGGACTAGCGGGAGCAGCATATCCTTTTGTGGTGTAAACAGTGA11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTCCGGAGAATCTCGTA11.3513513513513513TruSeq Adapter, Index 12 (97% over 35bp)
AGAGTTTTTCAAGAGGAAACACTCTGATGAAGTATCCATGTGAAATGGAT11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCGTCTCTCCTGAGACTATCCCGTAT11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCACGCATTCGTTCCCCGTATG11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTGTCATTAAATAAGCTGAAA11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTACCATTACTTTATCTTAAC11.3513513513513513No Hit
TCCGAGAGCTGGTACAATCTCCTGTTGGACATGCAGAATCGACTCAATAA11.3513513513513513No Hit
GAACTTGTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCT11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCAGCAATCTCGTAT11.3513513513513513TruSeq Adapter, Index 27 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCATCACTAATTA11.3513513513513513No Hit
CCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAA11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGCCATCTTATTATAGGGTAGAGGAGAGTG11.3513513513513513No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGCGATCTCGTAT11.3513513513513513TruSeq Adapter, Index 19 (97% over 38bp)
ATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTC11.3513513513513513No Hit
AAGGACAAGGGTTGCTTATGAAAGAATGTGCAATATCCTCAAAGGAAAAT11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCACGAAGGTCACAATTCCTT11.3513513513513513No Hit
GTCGCTTGGTCAGCAAGTGCTTGTCATGATGGCATCAATTGGCTAACAAT11.3513513513513513No Hit
AACTGTAGCAAGGTGTTTTTAAAATCCTATTAATTTTTGCTTTTTTAAAT11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCA50.0245.000027
GATCGGA50.0245.000021
GAAGAGC50.0245.000026
TCGGAAG50.0245.000023
GAGCACA50.0245.000029
CGGAAGA50.0245.000024
AGAGCAC50.0245.000028
ATCGGAA50.0245.000022
GGAAGAG50.0245.000025
ACCTCTC50.0245.00002245
AAAAAAA450.027.22222170-74
Produced by FastQC (version 0.11.2)