FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated g4.341000000085d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated g4.341000000085d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129489
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA44063.4026056267327727No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC37312.8813258269042157No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG36792.841167975658164No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG23711.8310435635459383No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT22221.7159758743985973No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG19331.4927908934349636No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC18541.4317818501957695No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG17701.3669114751059936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT17411.3445157503726186TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT11490.8873340592637212No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA10920.8433148761670876No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG8380.6471592181575269No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC8280.6394365544563632No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA7830.6046845678011259No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA7060.5452200573021646No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5590.43169690089505675No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG4540.3506089320328368No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4440.34288626833167296No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4140.3197182772281816No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3210.24789750480735817No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3140.24249164021654346No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA1660.12819621743931917No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT1580.12201808647838812No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA1350.10425595996571137No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGC151.5162663E-5245.08
CGGGTTA18800.0243.696811
GGGTTAT18850.0243.050382
GGGGGGA18150.0238.925611
GGTTATT19200.0237.981773
ATTAGTA19300.0236.74877
TATTAGT19300.0236.74876
TTATTAG19350.0236.136955
GTTATTA19750.0231.354424
TAGTAGA20050.0228.503749
GGAGCGA13000.0222.384635
GAGCGAA13050.0221.532586
GGGGGAG19650.0220.687042
AGCGAAA13000.0219.55777
CGAAAGC13000.0218.615399
GCGAAAG13050.0217.777798
GGGGAGC20800.0207.896643
GGGAGCG14450.0200.069214
TTAGTAG23050.0198.232128
CGAGAGG3000.0183.75245
Produced by FastQC (version 0.11.2)