FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated g3.34100000008553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated g3.34100000008553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293516
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT107103.6488641164365827TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA43151.4701072513934506No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC26790.9127270745036047No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG20610.7021763719865357No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG15850.5400046334782431No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG15830.5393232396189646No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG13750.4684582782539964No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC12220.41633164801918804No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA12020.40951770942640264No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11520.3924828629444392No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC11510.39214216601479984No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC11380.3877131059294893No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11230.3826026519849003No Hit
CGGGTTATTAGTAGAAACAAGGTTCTATCAAATTACAGTCCCACCAATGA10150.34580738358385915No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT10020.34137832349854863No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA7370.25109363714414207No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA7200.24530178934027447No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC7200.24530178934027447No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG7070.24087272925496397No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT6660.22690415513975387No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG6220.211913490235626No Hit
CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT6180.21055070251706892No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA5800.19760421919077664No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGTATTTGTACTGTGACTGGCTCCC5220.177843797271699No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5120.17443682797530627No Hit
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA5030.17137055560855285No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA4990.17000776788999578No Hit
CGGGTTATTAGTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCC4860.16557870780468525No Hit
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA4590.15637989070442498No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4540.15467640605622862No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4480.152632224478393No Hit
CGGGTTATTAGTAGAAACAAGGGATTAAAAATATTAAATGATAAGATAAA4430.15092873983019664No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC4200.14309271044849345No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA4010.1366194687853473No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3870.13184971177039753No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3770.12844274247400483No Hit
CGGGTTATTAGTAGAAACAAGGGAATAAAAAGTCACCAACTTAACTGGGT3720.12673925782580847No Hit
CGGGTTATTAGTAGAAACAAGGGGAAGAAGAGACTGTTCCGGGAATAGAA3690.12571716703689068No Hit
GGGGGGAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT3680.1253764701072514No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3680.1253764701072514No Hit
GGGGGGAGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTT3590.12231019774049795No Hit
CGGGTTATTAGTAGAAACAAGGAAAAGAATATGAATGAATGATCAGCTCA3530.12026601616266235No Hit
GGGGGGAGCAAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA3420.11651834993663038No Hit
CGGGTTATTAGTAGAAACAAGGATTTGCTACTAGAATATCAAGAGACCCC3070.10459395739925592No Hit
CGGGTTATTAGTAGAAACAAGGAGGGGAAAACAAGGTTAAAAACTGGTCA2990.10186838196214176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA42950.0243.859151
TTATTAG44950.0232.736375
GGGTTAT45600.0229.956152
GGTTATT45700.0229.18493
GGGGGGA53000.0227.433981
GTTATTA46150.0226.950164
TATTAGT46500.0226.032266
ATTAGTA47300.0221.691337
TAGTAGA47850.0218.631139
TTAGTAG50350.0207.775598
GGGAGCG35100.0196.13964
GGAGCGA35500.0193.92965
AGCGAAA36000.0189.875027
GAGCGAA36600.0187.76646
GCGAAAG37000.0184.412168
GGGGGAG66450.0180.662142
GGGGAGC66850.0179.214663
CGAAAGC38250.0178.065379
GCAAAAG31400.0159.952228
GAGCAAA31800.0159.866356
Produced by FastQC (version 0.11.2)