FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated g2.341000000084da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated g2.341000000084da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378116
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT5869115.521956225073788TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA36230.9581715663976134No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC34980.9251129283077151No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG23160.6125104465296364No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA19010.5027557680711738No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG17700.46811031535296044No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG15620.4131007415713696No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA14530.3842736091569783No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG13770.3641739571983201No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG13270.3509505019623607No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC12460.3295285044801066No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT10810.2858911022014408No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA9730.25732843889176865No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA8650.22876577558209651No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC8450.22347639348771278No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG8360.22109617154524008No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA7490.19808735943467082No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC7000.18512837330343068No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT6110.16159062298342308TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5350.14149097102476488No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT5340.1412265019200457No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG5170.13673052713981954No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4710.12456494832273693No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA4440.11742428249531889No Hit
CGGGTTATTAGTAGAAACAAGGGTTAGAAGATTTCAGTTGGATTTCTGAA4380.11583746786700377No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA4260.11266383861037353No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4110.10869680203958573No Hit
CGGGTTATTAGTAGAAACAAGGTCCCCGCAGAGCAAGGGGCTCCATCCTA4080.10790339472542818No Hit
CGGGTTATTAGTAGAAACAAGGGAAGAACACTGAGGAACACCAGAAGGTA3920.1036718890499212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA32500.0240.11
GGGTTAT33250.0235.052642
TATTAGT34950.0223.619456
TTATTAG34950.0223.268945
ATTAGTA35050.0222.631967
GTTATTA35400.0221.12294
TAGTAGA35750.0217.930089
GGTTATT36200.0216.236193
GGGGGGA56850.0208.799481
TTAGTAG38200.0203.952888
GGAGCGA35450.0203.878715
AGCGAAA36150.0200.269717
GAGCGAA36300.0200.117076
CGAAAGC36800.0197.065229
GCGAAAG36750.0197.08
GGGAGCG36650.0196.869034
GGGGAGC61200.0191.156053
GGGGGAG62550.0188.988832
GAGCAAA27500.0159.027276
GGAGCAA28700.0156.219515
Produced by FastQC (version 0.11.2)