FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated g1.34100000008454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated g1.34100000008454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232713
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3064413.168151328030664TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC46231.9865671449381856No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC33591.4434088340574012No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA30111.2938684130237676No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG24111.0360401008968128No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG19450.8357934451448781No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG19250.8271991680739795No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT16080.6909798765002385TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG15320.6583216236308242No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA14660.6299605092968592No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA14520.6239445153472303No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT13900.5973022564274449No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC11010.4731149527529618No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG10090.43358127822682874No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG9390.40350130847868404No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5860.25181231817732574No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5720.24579632422769676No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5660.24321804110642725No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA5060.21743520989373177No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATTTCGTAT3510.1508295625942685TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3180.13664900542728597No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3100.13321129459892658No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC3060.13149243918474687TruSeq Adapter, Index 27 (97% over 36bp)
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA2920.12547644523511792No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG2740.11774159587130929TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA20900.0243.827761
GGGTTAT20950.0243.245832
GGGGGGA12100.0239.938031
GGTTATT21550.0236.473333
TTATTAG22400.0227.55
ATTAGTA22600.0223.860617
TATTAGT22750.0223.461556
GTTATTA23150.0220.12964
TAGTAGA23600.0214.3759
GCGAAAG8800.0214.374988
GGAGCGA8850.0213.163835
GAGCGAA8950.0210.782126
AGCGAAA8950.0210.782127
CGAAAGC9050.0208.453059
TTAGTAG24300.0208.199588
GGGGGAG14250.0202.877182
GGGGAGC15950.0181.253923
GGGAGCG10650.0177.136154
CGGGTTT150.004964432163.333331
CGAGAGG3950.0133.35443245
Produced by FastQC (version 0.11.2)