##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE52Y l01n01 flu mrt-pcr updated f7.34100000008744.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76
Sequences flagged as poor quality	0
Sequence length	251
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18421052631579	0.0	0.0	0.0	0.0	0.0
2	32.69736842105263	0.0	0.0	0.0	0.0	0.0
3	32.421052631578945	0.0	0.0	0.0	0.0	0.0
4	32.75	0.0	0.0	0.0	0.0	0.0
5	32.06578947368421	0.0	0.0	0.0	0.0	0.0
6	33.30263157894737	0.0	0.0	0.0	0.0	0.0
7	33.98684210526316	0.0	0.0	0.0	0.0	0.0
8	34.76315789473684	0.0	0.0	0.0	0.0	0.0
9	34.3421052631579	0.0	0.0	0.0	0.0	0.0
10-14	35.47894736842105	0.0	0.0	0.0	0.0	0.0
15-19	36.4578947368421	0.0	0.0	0.0	0.0	0.0
20-24	35.247368421052634	0.0	0.0	0.0	0.0	0.0
25-29	35.56842105263158	0.0	0.0	0.0	0.0	0.0
30-34	33.565789473684205	0.0	0.0	0.0	0.0	0.0
35-39	31.334210526315793	0.0	0.0	0.0	0.0	0.0
40-44	31.563157894736843	0.0	0.0	0.0	0.0	0.0
45-49	32.868421052631575	0.0	0.0	0.0	0.0	0.0
50-54	32.921052631578945	0.0	0.0	0.0	0.0	0.0
55-59	33.22631578947368	0.0	0.0	0.0	0.0	0.0
60-64	32.60263157894737	0.0	0.0	0.0	0.0	0.0
65-69	32.61052631578948	0.0	0.0	0.0	0.0	0.0
70-74	33.20263157894737	0.0	0.0	0.0	0.0	0.0
75-79	29.376315789473683	0.0	0.0	0.0	0.0	0.0
80-84	29.20526315789474	0.0	0.0	0.0	0.0	0.0
85-89	30.113157894736837	0.0	0.0	0.0	0.0	0.0
90-94	28.818421052631578	0.0	0.0	0.0	0.0	0.0
95-99	29.771052631578947	0.0	0.0	0.0	0.0	0.0
100-104	28.97105263157895	0.0	0.0	0.0	0.0	0.0
105-109	28.807894736842105	0.0	0.0	0.0	0.0	0.0
110-114	29.121052631578948	0.0	0.0	0.0	0.0	0.0
115-119	28.72631578947368	0.0	0.0	0.0	0.0	0.0
120-124	28.389473684210525	0.0	0.0	0.0	0.0	0.0
125-129	29.21052631578948	0.0	0.0	0.0	0.0	0.0
130-134	27.80526315789474	0.0	0.0	0.0	0.0	0.0
135-139	28.139473684210525	0.0	0.0	0.0	0.0	0.0
140-144	28.410526315789475	0.0	0.0	0.0	0.0	0.0
145-149	27.47105263157895	0.0	0.0	0.0	0.0	0.0
150-154	27.931578947368422	0.0	0.0	0.0	0.0	0.0
155-159	28.49473684210526	0.0	0.0	0.0	0.0	0.0
160-164	27.363157894736844	0.0	0.0	0.0	0.0	0.0
165-169	27.55	0.0	0.0	0.0	0.0	0.0
170-174	27.55	0.0	0.0	0.0	0.0	0.0
175-179	27.292105263157897	0.0	0.0	0.0	0.0	0.0
180-184	25.65	0.0	0.0	0.0	0.0	0.0
185-189	26.85263157894737	0.0	0.0	0.0	0.0	0.0
190-194	27.405263157894733	0.0	0.0	0.0	0.0	0.0
195-199	26.742105263157896	0.0	0.0	0.0	0.0	0.0
200-204	27.178947368421053	0.0	0.0	0.0	0.0	0.0
205-209	26.136842105263156	0.0	0.0	0.0	0.0	0.0
210-214	25.80263157894737	0.0	0.0	0.0	0.0	0.0
215-219	25.61578947368421	0.0	0.0	0.0	0.0	0.0
220-224	25.092105263157894	0.0	0.0	0.0	0.0	0.0
225-229	23.910526315789475	0.0	0.0	0.0	0.0	0.0
230-234	24.43947368421053	0.0	0.0	0.0	0.0	0.0
235-239	23.76578947368421	0.0	0.0	0.0	0.0	0.0
240-244	22.468421052631577	0.0	0.0	0.0	0.0	0.0
245-249	22.823684210526316	0.0	0.0	0.0	0.0	0.0
250-251	21.57236842105263	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1103	1	1.5714285714285694
1103	2	1.5714285714285694
1103	3	2.2857142857142847
1103	4	1.4285714285714306
1103	5	2.2857142857142847
1103	6	0.8571428571428541
1103	7	0.1428571428571459
1103	8	0.2857142857142847
1103	9	1.142857142857146
1103	10-14	0.17142857142856371
1103	15-19	0.11428571428571388
1103	20-24	3.5999999999999943
1103	25-29	3.3999999999999986
1103	30-34	7.828571428571426
1103	35-39	-10.714285714285715
1103	40-44	4.485714285714291
1103	45-49	8.200000000000003
1103	50-54	8.514285714285712
1103	55-59	5.857142857142854
1103	60-64	9.25714285714286
1103	65-69	6.5428571428571445
1103	70-74	4.885714285714293
1103	75-79	-2.457142857142859
1103	80-84	-4.171428571428578
1103	85-89	-4.257142857142856
1103	90-94	-5.342857142857142
1103	95-99	-2.799999999999997
1103	100-104	-2.857142857142861
1103	105-109	-4.514285714285716
1103	110-114	-6.057142857142853
1103	115-119	-6.742857142857144
1103	120-124	-5.371428571428574
1103	125-129	-8.600000000000001
1103	130-134	-5.342857142857145
1103	135-139	-7.485714285714284
1103	140-144	-7.057142857142857
1103	145-149	-4.31428571428571
1103	150-154	-4.057142857142853
1103	155-159	-6.314285714285713
1103	160-164	-1.1142857142857139
1103	165-169	-3.37142857142857
1103	170-174	-6.085714285714285
1103	175-179	-6.657142857142858
1103	180-184	-0.7142857142857117
1103	185-189	-6.114285714285717
1103	190-194	-5.342857142857142
1103	195-199	-10.514285714285718
1103	200-204	-4.914285714285715
1103	205-209	-9.200000000000003
1103	210-214	-4.628571428571426
1103	215-219	-2.799999999999997
1103	220-224	-7.342857142857143
1103	225-229	-3.8857142857142826
1103	230-234	-10.171428571428574
1103	235-239	-6.485714285714282
1103	240-244	-7.371428571428574
1103	245-249	-11.571428571428575
1103	250-251	-0.35714285714285765
2112	1	2.5714285714285694
2112	2	2.5714285714285694
2112	3	2.2857142857142847
2112	4	2.4285714285714306
2112	5	2.2857142857142847
2112	6	-0.1428571428571459
2112	7	-0.8571428571428541
2112	8	-1.7142857142857153
2112	9	0.1428571428571459
2112	10-14	0.17142857142856371
2112	15-19	0.11428571428571388
2112	20-24	3.5999999999999943
2112	25-29	2.1999999999999957
2112	30-34	-10.171428571428574
2112	35-39	-7.914285714285715
2112	40-44	-8.514285714285709
2112	45-49	-12.799999999999997
2112	50-54	-11.085714285714285
2112	55-59	-11.742857142857144
2112	60-64	-8.342857142857142
2112	65-69	0.5428571428571445
2112	70-74	2.6857142857142904
2112	75-79	-4.25714285714286
2112	80-84	-7.971428571428575
2112	85-89	-5.4571428571428555
2112	90-94	-6.542857142857141
2112	95-99	-6.9999999999999964
2112	100-104	-7.657142857142862
2112	105-109	-4.114285714285717
2112	110-114	-5.657142857142855
2112	115-119	-3.142857142857146
2112	120-124	-5.371428571428574
2112	125-129	-8.600000000000001
2112	130-134	-7.5428571428571445
2112	135-139	-5.285714285714285
2112	140-144	-6.4571428571428555
2112	145-149	-4.9142857142857075
2112	150-154	-5.257142857142856
2112	155-159	-8.914285714285713
2112	160-164	-6.714285714285712
2112	165-169	-3.37142857142857
2112	170-174	-4.485714285714284
2112	175-179	-8.25714285714286
2112	180-184	-6.514285714285711
2112	185-189	-3.1142857142857174
2112	190-194	-9.342857142857142
2112	195-199	-6.914285714285718
2112	200-204	-5.914285714285715
2112	205-209	-6.200000000000003
2112	210-214	-6.428571428571427
2112	215-219	-3.599999999999998
2112	220-224	-4.942857142857143
2112	225-229	-5.685714285714283
2112	230-234	-7.171428571428574
2112	235-239	-4.085714285714282
2112	240-244	-6.171428571428571
2112	245-249	-1.971428571428575
2112	250-251	-1.3571428571428577
1113	1	-0.4285714285714306
1113	2	-0.4285714285714306
1113	3	-0.7142857142857153
1113	4	-0.5714285714285694
1113	5	-0.7142857142857153
1113	6	-1.142857142857146
1113	7	-0.8571428571428541
1113	8	-1.7142857142857153
1113	9	-0.8571428571428541
1113	10-14	0.17142857142856371
1113	15-19	0.11428571428571388
1113	20-24	2.999999999999993
1113	25-29	3.0
1113	30-34	2.628571428571423
1113	35-39	4.4857142857142875
1113	40-44	-0.9142857142857075
1113	45-49	7.399999999999999
1113	50-54	8.314285714285717
1113	55-59	5.257142857142853
1113	60-64	8.857142857142861
1113	65-69	5.942857142857143
1113	70-74	3.6857142857142904
1113	75-79	-7.25714285714286
1113	80-84	-10.371428571428577
1113	85-89	-7.257142857142856
1113	90-94	-1.1428571428571423
1113	95-99	-1.5999999999999943
1113	100-104	0.1428571428571388
1113	105-109	-8.114285714285717
1113	110-114	-2.4571428571428555
1113	115-119	-5.142857142857146
1113	120-124	0.22857142857142776
1113	125-129	-3.1999999999999993
1113	130-134	-0.3428571428571452
1113	135-139	-3.6857142857142833
1113	140-144	2.5428571428571445
1113	145-149	-4.714285714285708
1113	150-154	-9.057142857142855
1113	155-159	-5.914285714285711
1113	160-164	-7.5142857142857125
1113	165-169	-7.7714285714285705
1113	170-174	-9.885714285714284
1113	175-179	-10.05714285714286
1113	180-184	-4.5142857142857125
1113	185-189	-4.914285714285718
1113	190-194	-2.3428571428571416
1113	195-199	-1.3142857142857167
1113	200-204	-6.114285714285714
1113	205-209	-7.8000000000000025
1113	210-214	-8.228571428571428
1113	215-219	-5.199999999999999
1113	220-224	4.657142857142858
1113	225-229	-2.2857142857142847
1113	230-234	-1.3714285714285737
1113	235-239	-8.485714285714282
1113	240-244	-7.9714285714285715
1113	245-249	-8.171428571428574
1113	250-251	-1.8571428571428577
2102	1	-1.4285714285714306
2102	2	-1.4285714285714306
2102	3	-1.7142857142857153
2102	4	-1.5714285714285694
2102	5	-1.7142857142857153
2102	6	-3.142857142857146
2102	7	-2.857142857142854
2102	8	1.2857142857142847
2102	9	2.142857142857146
2102	10-14	0.17142857142856371
2102	15-19	0.11428571428571388
2102	20-24	-5.0000000000000036
2102	25-29	-0.8000000000000043
2102	30-34	-1.571428571428573
2102	35-39	-5.914285714285715
2102	40-44	-4.31428571428571
2102	45-49	-8.2
2102	50-54	-8.485714285714284
2102	55-59	-6.942857142857143
2102	60-64	-8.74285714285714
2102	65-69	-14.857142857142854
2102	70-74	-3.914285714285711
2102	75-79	-7.857142857142861
2102	80-84	1.028571428571425
2102	85-89	3.5428571428571445
2102	90-94	-3.542857142857141
2102	95-99	-3.799999999999997
2102	100-104	-3.4571428571428626
2102	105-109	-1.3142857142857167
2102	110-114	-1.0571428571428534
2102	115-119	-8.742857142857144
2102	120-124	-1.7714285714285722
2102	125-129	3.0
2102	130-134	-0.7428571428571438
2102	135-139	-2.6857142857142833
2102	140-144	-3.6571428571428584
2102	145-149	-3.714285714285708
2102	150-154	-1.4571428571428555
2102	155-159	3.885714285714286
2102	160-164	-6.114285714285714
2102	165-169	-5.9714285714285715
2102	170-174	-2.6857142857142833
2102	175-179	-4.25714285714286
2102	180-184	-6.11428571428571
2102	185-189	-3.714285714285719
2102	190-194	-2.1428571428571423
2102	195-199	-3.1142857142857174
2102	200-204	-2.314285714285713
2102	205-209	-6.400000000000002
2102	210-214	-5.428571428571427
2102	215-219	-7.999999999999998
2102	220-224	-6.342857142857143
2102	225-229	-3.0857142857142854
2102	230-234	-5.571428571428573
2102	235-239	-4.885714285714283
2102	240-244	-4.771428571428572
2102	245-249	2.8285714285714256
2102	250-251	-7.857142857142858
2107	1	-1.4285714285714306
2107	2	-1.4285714285714306
2107	3	-1.7142857142857153
2107	4	-1.5714285714285694
2107	5	-1.7142857142857153
2107	6	-0.1428571428571459
2107	7	0.1428571428571459
2107	8	-0.7142857142857153
2107	9	-2.857142857142854
2107	10-14	-1.028571428571432
2107	15-19	-0.6857142857142833
2107	20-24	-12.000000000000004
2107	25-29	-11.8
2107	30-34	-8.771428571428576
2107	35-39	3.2857142857142847
2107	40-44	-9.11428571428571
2107	45-49	-10.2
2107	50-54	-13.485714285714284
2107	55-59	-2.7428571428571438
2107	60-64	-13.74285714285714
2107	65-69	-9.857142857142854
2107	70-74	-17.11428571428571
2107	75-79	-4.857142857142861
2107	80-84	-5.371428571428577
2107	85-89	-7.857142857142858
2107	90-94	-8.342857142857142
2107	95-99	-3.399999999999995
2107	100-104	-8.457142857142863
2107	105-109	-8.914285714285718
2107	110-114	-9.057142857142853
2107	115-119	-4.5428571428571445
2107	120-124	-6.771428571428572
2107	125-129	-9.200000000000001
2107	130-134	-9.542857142857144
2107	135-139	-5.485714285714284
2107	140-144	-4.857142857142858
2107	145-149	-5.714285714285708
2107	150-154	-4.4571428571428555
2107	155-159	-7.314285714285713
2107	160-164	-3.114285714285714
2107	165-169	-7.37142857142857
2107	170-174	-2.6857142857142833
2107	175-179	1.7428571428571402
2107	180-184	-4.914285714285711
2107	185-189	-2.914285714285718
2107	190-194	-4.742857142857144
2107	195-199	-5.914285714285718
2107	200-204	-7.114285714285714
2107	205-209	3.3999999999999986
2107	210-214	-1.428571428571427
2107	215-219	-5.9999999999999964
2107	220-224	-5.342857142857143
2107	225-229	-6.885714285714284
2107	230-234	-0.37142857142857366
2107	235-239	-5.885714285714283
2107	240-244	4.428571428571427
2107	245-249	-5.171428571428574
2107	250-251	-2.8571428571428577
2105	1	-1.4285714285714306
2105	2	-1.4285714285714306
2105	3	-0.7142857142857153
2105	4	-0.5714285714285694
2105	5	-0.7142857142857153
2105	6	1.857142857142854
2105	7	2.142857142857146
2105	8	1.2857142857142847
2105	9	-1.857142857142854
2105	10-14	0.17142857142856371
2105	15-19	0.11428571428571388
2105	20-24	3.1999999999999957
2105	25-29	2.6000000000000014
2105	30-34	2.8285714285714256
2105	35-39	8.085714285714282
2105	40-44	8.685714285714294
2105	45-49	7.399999999999999
2105	50-54	7.5142857142857125
2105	55-59	4.057142857142857
2105	60-64	4.25714285714286
2105	65-69	5.5428571428571445
2105	70-74	4.085714285714289
2105	75-79	11.942857142857143
2105	80-84	13.028571428571425
2105	85-89	9.942857142857143
2105	90-94	12.257142857142856
2105	95-99	8.800000000000004
2105	100-104	8.142857142857135
2105	105-109	13.085714285714285
2105	110-114	11.942857142857147
2105	115-119	14.057142857142857
2105	120-124	11.028571428571425
2105	125-129	11.999999999999996
2105	130-134	7.857142857142854
2105	135-139	9.314285714285713
2105	140-144	6.5428571428571445
2105	145-149	10.685714285714294
2105	150-154	7.5428571428571445
2105	155-159	11.085714285714289
2105	160-164	8.285714285714288
2105	165-169	12.62857142857143
2105	170-174	11.714285714285715
2105	175-179	12.74285714285714
2105	180-184	4.88571428571429
2105	185-189	10.68571428571428
2105	190-194	10.857142857142858
2105	195-199	11.885714285714283
2105	200-204	12.285714285714288
2105	205-209	12.8
2105	210-214	12.571428571428573
2105	215-219	13.400000000000002
2105	220-224	3.6571428571428584
2105	225-229	6.314285714285717
2105	230-234	9.228571428571428
2105	235-239	14.114285714285717
2105	240-244	7.628571428571426
2105	245-249	11.828571428571426
2105	250-251	0.6428571428571423
1110	1	0.5714285714285694
1110	2	0.5714285714285694
1110	3	0.2857142857142847
1110	4	0.4285714285714306
1110	5	0.2857142857142847
1110	6	1.857142857142854
1110	7	2.142857142857146
1110	8	1.2857142857142847
1110	9	2.142857142857146
1110	10-14	0.17142857142856371
1110	15-19	0.11428571428571388
1110	20-24	3.5999999999999943
1110	25-29	1.3999999999999986
1110	30-34	7.228571428571424
1110	35-39	8.685714285714283
1110	40-44	9.685714285714294
1110	45-49	8.200000000000003
1110	50-54	8.714285714285715
1110	55-59	6.257142857142853
1110	60-64	8.457142857142863
1110	65-69	6.142857142857146
1110	70-74	5.68571428571429
1110	75-79	14.74285714285714
1110	80-84	13.828571428571422
1110	85-89	11.342857142857142
1110	90-94	12.657142857142855
1110	95-99	9.800000000000004
1110	100-104	14.142857142857135
1110	105-109	13.885714285714283
1110	110-114	12.342857142857145
1110	115-119	14.257142857142853
1110	120-124	8.028571428571428
1110	125-129	14.599999999999998
1110	130-134	15.657142857142855
1110	135-139	15.314285714285713
1110	140-144	12.942857142857143
1110	145-149	12.685714285714294
1110	150-154	16.742857142857144
1110	155-159	13.485714285714288
1110	160-164	16.285714285714285
1110	165-169	15.228571428571431
1110	170-174	14.114285714285714
1110	175-179	14.74285714285714
1110	180-184	17.885714285714286
1110	185-189	10.085714285714285
1110	190-194	13.05714285714286
1110	195-199	15.885714285714283
1110	200-204	14.085714285714285
1110	205-209	13.399999999999995
1110	210-214	13.571428571428573
1110	215-219	12.2
1110	220-224	15.657142857142858
1110	225-229	15.514285714285716
1110	230-234	15.428571428571423
1110	235-239	15.714285714285719
1110	240-244	14.228571428571428
1110	245-249	12.228571428571424
1110	250-251	13.642857142857142
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
20	3.0
21	4.0
22	10.0
23	9.0
24	6.0
25	2.0
26	3.0
27	1.0
28	1.0
29	2.0
30	4.0
31	1.0
32	5.0
33	2.0
34	2.0
35	6.0
36	5.0
37	3.0
38	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10526315789473	14.473684210526317	6.578947368421052	36.84210526315789
2	43.42105263157895	39.473684210526315	7.894736842105263	9.210526315789473
3	42.10526315789473	15.789473684210526	32.89473684210527	9.210526315789473
4	39.473684210526315	11.842105263157894	13.157894736842104	35.526315789473685
5	40.78947368421053	9.210526315789473	39.473684210526315	10.526315789473683
6	42.10526315789473	9.210526315789473	39.473684210526315	9.210526315789473
7	5.263157894736842	67.10526315789474	15.789473684210526	11.842105263157894
8	19.736842105263158	35.526315789473685	40.78947368421053	3.9473684210526314
9	32.89473684210527	10.526315789473683	38.15789473684211	18.421052631578945
10-14	27.105263157894736	40.78947368421053	15.0	17.105263157894736
15-19	16.05263157894737	39.473684210526315	18.157894736842106	26.31578947368421
20-24	28.421052631578945	31.57894736842105	24.210526315789473	15.789473684210526
25-29	18.684210526315788	21.842105263157897	41.31578947368421	18.157894736842106
30-34	10.263157894736842	30.789473684210527	30.526315789473685	28.421052631578945
35-39	22.894736842105264	31.31578947368421	28.947368421052634	16.842105263157894
40-44	13.684210526315791	31.05263157894737	29.47368421052631	25.789473684210527
45-49	20.263157894736842	25.789473684210527	32.10526315789474	21.842105263157897
50-54	28.947368421052634	25.263157894736842	20.526315789473685	25.263157894736842
55-59	12.894736842105264	17.36842105263158	39.473684210526315	30.263157894736842
60-64	22.105263157894736	20.0	37.10526315789473	20.789473684210527
65-69	15.263157894736842	45.78947368421053	19.736842105263158	19.210526315789473
70-74	13.157894736842104	46.31578947368421	22.63157894736842	17.894736842105264
75-79	15.263157894736842	35.26315789473684	25.263157894736842	24.210526315789473
80-84	21.052631578947366	29.210526315789476	25.526315789473685	24.210526315789473
85-89	20.263157894736842	24.736842105263158	26.842105263157894	28.157894736842103
90-94	18.947368421052634	27.89473684210526	29.736842105263158	23.42105263157895
95-99	23.94736842105263	28.421052631578945	27.89473684210526	19.736842105263158
100-104	20.263157894736842	23.684210526315788	28.157894736842103	27.89473684210526
105-109	21.842105263157897	28.68421052631579	23.42105263157895	26.052631578947366
110-114	18.684210526315788	25.0	35.78947368421053	20.526315789473685
115-119	20.526315789473685	27.105263157894736	32.631578947368425	19.736842105263158
120-124	18.684210526315788	23.42105263157895	27.89473684210526	30.0
125-129	14.473684210526317	22.894736842105264	30.263157894736842	32.36842105263158
130-134	22.105263157894736	25.263157894736842	28.947368421052634	23.684210526315788
135-139	18.684210526315788	20.263157894736842	25.526315789473685	35.526315789473685
140-144	17.105263157894736	26.57894736842105	27.631578947368425	28.68421052631579
145-149	20.789473684210527	23.94736842105263	30.263157894736842	25.0
150-154	15.789473684210526	30.0	19.473684210526315	34.73684210526316
155-159	17.894736842105264	26.31578947368421	31.57894736842105	24.210526315789473
160-164	19.473684210526315	36.84210526315789	20.0	23.684210526315788
165-169	17.36842105263158	26.31578947368421	26.052631578947366	30.263157894736842
170-174	18.947368421052634	26.052631578947366	28.947368421052634	26.052631578947366
175-179	13.421052631578947	29.210526315789476	30.263157894736842	27.105263157894736
180-184	16.315789473684212	26.052631578947366	30.0	27.631578947368425
185-189	13.421052631578947	21.31578947368421	31.842105263157894	33.421052631578945
190-194	14.736842105263156	26.842105263157894	32.36842105263158	26.052631578947366
195-199	15.526315789473685	32.10526315789474	31.05263157894737	21.31578947368421
200-204	15.0	23.157894736842106	29.47368421052631	32.36842105263158
205-209	16.842105263157894	23.684210526315788	33.421052631578945	26.052631578947366
210-214	19.473684210526315	22.894736842105264	31.05263157894737	26.57894736842105
215-219	17.36842105263158	31.31578947368421	27.368421052631582	23.94736842105263
220-224	15.789473684210526	29.210526315789476	26.57894736842105	28.421052631578945
225-229	19.736842105263158	23.684210526315788	29.736842105263158	26.842105263157894
230-234	18.684210526315788	25.789473684210527	27.89473684210526	27.631578947368425
235-239	17.63157894736842	28.68421052631579	27.368421052631582	26.31578947368421
240-244	19.210526315789473	31.31578947368421	23.94736842105263	25.526315789473685
245-249	17.894736842105264	29.47368421052631	29.47368421052631	23.157894736842106
250-251	14.473684210526317	22.36842105263158	29.605263157894733	33.55263157894737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.3333333333333333
31	0.3333333333333333
32	0.0
33	0.0
34	0.0
35	1.0
36	1.0
37	0.6666666666666666
38	1.0
39	1.9999999999999998
40	1.3333333333333333
41	3.0
42	3.0
43	2.333333333333333
44	2.333333333333333
45	3.666666666666667
46	4.0
47	3.3333333333333335
48	1.6666666666666665
49	2.6666666666666665
50	1.6666666666666665
51	0.3333333333333333
52	0.6666666666666666
53	0.6666666666666666
54	0.3333333333333333
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	76.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.42105263157895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.36619718309859	88.1578947368421
2	4.225352112676056	7.894736842105263
3	1.4084507042253522	3.9473684210526314
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	3	3.9473684210526314	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCAATCTCGTAT	2	2.631578947368421	TruSeq Adapter, Index 19 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	2	2.631578947368421	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2	2.631578947368421	No Hit
CATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGG	1	1.3157894736842104	No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCCACATTCAGAAATCTCGTAT	1	1.3157894736842104	No Hit
TCTATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTGGAA	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGGACTTTTTTGTCCTGAATGAATAGGAAA	1	1.3157894736842104	No Hit
ACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGTCTTGTCTT	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACTCTCGTATG	1	1.3157894736842104	TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGAGATATTTAAATATGAGACTTCTAACCGAGG	1	1.3157894736842104	No Hit
GGGGGGAGCAAAAGCAGGGTAAATAAAAACTCATTGACTCCAACACAATG	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAAGATATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCACTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTGAAGCTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTAGTTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGGTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 7 (97% over 38bp)
TCCTCAGGTAGTGGTCCATCAATCGGGTTGAGCTGGGGTGCACCAGTCTC	1	1.3157894736842104	No Hit
AAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAACTTCTGTTCGCAT	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGCACATTCAGAAATCTCGTATG	1	1.3157894736842104	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGCAAATATGACCTTTAATTTCAGCACGTAGAACAGTGCCTGGCAGAGAG	1	1.3157894736842104	No Hit
CTACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGC	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCATCACGATGTCGTAATTCGGATG	1	1.3157894736842104	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAACTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 15 (97% over 37bp)
ACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCATGAGG	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGCTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAATTCAGAAATCTCGTATG	1	1.3157894736842104	TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAAGCTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTAAAAAATTAGAGACAAAAAG	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTCTCATTAAATAAGCTGAAC	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	1	1.3157894736842104	No Hit
CATTTCAGAATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTA	1	1.3157894736842104	No Hit
AACAGTCTAACTGAAAGCTTGACATGGTGATGGAGCCCATTATGTTTTTG	1	1.3157894736842104	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 13 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTATCATAGCTCAATCTTGAC	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTAATCTAGCGCTATGTTGAC	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAAGCAATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 22 (97% over 37bp)
TGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTTCGAGAGT	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACATTTCGTCACTTATTG	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCTCATCAGTCATACTCCTC	1	1.3157894736842104	No Hit
GGGTTGCTTATGAAAGAATGTGCAATATCCTCAAAGGAAAATTTCAAACA	1	1.3157894736842104	No Hit
CTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCAT	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGCAGTTTTTTAATAGATCCCCACACAATG	1	1.3157894736842104	No Hit
ATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAA	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA	1	1.3157894736842104	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAGA	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACACTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 7 (97% over 37bp)
CAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTACCCCATCTATTTCTT	1	1.3157894736842104	No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA	1	1.3157894736842104	No Hit
AAAGCATTAGTTCAAGAAACGGTTTTGAGATGATTTGGGATCCGAACGGA	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAAGCAATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTAACTCATTCGACACTATTAG	1	1.3157894736842104	No Hit
GCGCCAAACTCTCCTTATTTCTTCTTTGTCATAAAGGCTGAGTTCTCTCA	1	1.3157894736842104	No Hit
GGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCA	1	1.3157894736842104	No Hit
AGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATT	1	1.3157894736842104	No Hit
CAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAGGGTT	1	1.3157894736842104	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT	1	1.3157894736842104	TruSeq Adapter, Index 19 (97% over 38bp)
TAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCT	1	1.3157894736842104	No Hit
AGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTG	1	1.3157894736842104	No Hit
CCACAAGAGGGGTCCAGATTGCTTCAAATGAGAATGTGGAAACCATGGAC	1	1.3157894736842104	No Hit
TTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCACT	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTAATATAGCTCCATTTTGAC	1	1.3157894736842104	No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG	1	1.3157894736842104	No Hit
GTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGA	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGGAATTTTTTCTCAACTCTCATACTCCTC	1	1.3157894736842104	No Hit
ATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGTTGCTTT	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTCATGCTTCTGATAGCATA	1	1.3157894736842104	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0	0.0	0.0
15-19	0.0	0.0	0.0
20-24	0.0	0.0	0.0
25-29	0.0	0.0	0.0
30-34	0.0	0.0	0.0
35-39	0.0	0.0	0.0
40-44	0.0	0.0	0.0
45-49	0.0	0.0	0.0
50-54	0.0	0.0	0.0
55-59	0.0	0.0	0.0
60-64	0.0	0.0	0.0
65-69	0.0	0.0	0.0
70-74	0.0	0.0	0.0
75-79	0.0	0.0	0.0
80-84	0.0	0.0	0.0
85-89	0.0	0.0	0.0
90-94	0.0	0.0	0.0
95-99	0.0	0.0	0.0
100-104	0.0	0.0	0.0
105-109	0.0	0.0	0.0
110-114	0.0	0.0	0.0
115-119	0.0	0.0	0.0
120-124	0.0	0.0	0.0
125-129	0.7894736842105263	0.0	0.0
130-134	1.3157894736842106	0.0	0.0
135-139	1.3157894736842106	0.0	0.0
140-144	1.3157894736842106	0.0	0.0
145-149	1.3157894736842106	0.0	0.0
150-154	3.421052631578948	0.0	0.0
155-159	6.578947368421052	0.0	0.0
160-164	6.842105263157895	0.0	0.0
165-169	8.157894736842106	0.0	0.0
170-174	9.210526315789474	0.0	0.0
175-179	11.05263157894737	0.0	0.0
180-184	11.842105263157896	0.0	0.0
185-189	11.842105263157896	0.0	0.0
190-194	13.157894736842104	0.0	0.0
195-199	16.315789473684212	0.0	0.0
200-204	17.631578947368418	0.0	0.0
205-209	19.736842105263158	0.0	0.0
210-214	19.736842105263158	0.0	0.0
215-219	21.315789473684212	0.0	0.0
220-224	22.36842105263158	0.0	0.0
225-229	22.36842105263158	0.0	0.0
230-234	25.0	0.0	0.0
235-239	25.263157894736842	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGAGCA	5	0.0	245.00002	4
GAGCAAA	5	0.0	245.00002	6
GGGGGGA	5	0.0	245.00002	1
GGGGGAG	5	0.0	245.00002	2
GCAAAAG	5	0.0	245.00002	8
AGCAAAA	5	0.0	245.00002	7
GGGGAGC	5	0.0	245.00002	3
GGAATCC	5	0.0	245.00002	245
GGAGCAA	5	0.0	245.00002	5
CAAAAGC	5	0.0	245.00002	9
>>END_MODULE