FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated f7.34100000008744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated f7.34100000008744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC33.9473684210526314No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCAATCTCGTAT22.631578947368421TruSeq Adapter, Index 19 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG22.631578947368421No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA22.631578947368421No Hit
CATAAGTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGG11.3157894736842104No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCCACATTCAGAAATCTCGTAT11.3157894736842104No Hit
TCTATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTGGAA11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGGACTTTTTTGTCCTGAATGAATAGGAAA11.3157894736842104No Hit
ACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGTCTTGTCTT11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACTCTCGTATG11.3157894736842104TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGAGATATTTAAATATGAGACTTCTAACCGAGG11.3157894736842104No Hit
GGGGGGAGCAAAAGCAGGGTAAATAAAAACTCATTGACTCCAACACAATG11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAAGATATCTCGTAT11.3157894736842104TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCACTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACTGAAGCTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTAGTTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGGTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 7 (97% over 38bp)
TCCTCAGGTAGTGGTCCATCAATCGGGTTGAGCTGGGGTGCACCAGTCTC11.3157894736842104No Hit
AAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAACTTCTGTTCGCAT11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGCACATTCAGAAATCTCGTATG11.3157894736842104Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGCAAATATGACCTTTAATTTCAGCACGTAGAACAGTGCCTGGCAGAGAG11.3157894736842104No Hit
CTACCTTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGC11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCATCACGATGTCGTAATTCGGATG11.3157894736842104Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAACTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 15 (97% over 37bp)
ACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCATGAGG11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT11.3157894736842104TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAAGCTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAATTCAGAAATCTCGTATG11.3157894736842104TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAAGCTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTAAAAAATTAGAGACAAAAAG11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTCTCATTAAATAAGCTGAAC11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC11.3157894736842104No Hit
CATTTCAGAATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTA11.3157894736842104No Hit
AACAGTCTAACTGAAAGCTTGACATGGTGATGGAGCCCATTATGTTTTTG11.3157894736842104No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT11.3157894736842104TruSeq Adapter, Index 13 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTATCATAGCTCAATCTTGAC11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTAATCTAGCGCTATGTTGAC11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAAGCAATCTCGTAT11.3157894736842104TruSeq Adapter, Index 22 (97% over 37bp)
TGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTTCGAGAGT11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACATTTCGTCACTTATTG11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCTCATCAGTCATACTCCTC11.3157894736842104No Hit
GGGTTGCTTATGAAAGAATGTGCAATATCCTCAAAGGAAAATTTCAAACA11.3157894736842104No Hit
CTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCAT11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGCAGTTTTTTAATAGATCCCCACACAATG11.3157894736842104No Hit
ATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAA11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA11.3157894736842104No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAGA11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACACTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 7 (97% over 37bp)
CAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTACCCCATCTATTTCTT11.3157894736842104No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA11.3157894736842104No Hit
AAAGCATTAGTTCAAGAAACGGTTTTGAGATGATTTGGGATCCGAACGGA11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAAGCAATCTCGTAT11.3157894736842104TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTAACTCATTCGACACTATTAG11.3157894736842104No Hit
GCGCCAAACTCTCCTTATTTCTTCTTTGTCATAAAGGCTGAGTTCTCTCA11.3157894736842104No Hit
GGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCAACTGTCGCCA11.3157894736842104No Hit
AGAAGCTGCTTTTGGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATT11.3157894736842104No Hit
CAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAGGGTT11.3157894736842104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT11.3157894736842104TruSeq Adapter, Index 19 (97% over 38bp)
TAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCT11.3157894736842104No Hit
AGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTG11.3157894736842104No Hit
CCACAAGAGGGGTCCAGATTGCTTCAAATGAGAATGTGGAAACCATGGAC11.3157894736842104No Hit
TTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCACT11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTAATATAGCTCCATTTTGAC11.3157894736842104No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG11.3157894736842104No Hit
GTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGA11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGGAATTTTTTCTCAACTCTCATACTCCTC11.3157894736842104No Hit
ATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGTTGCTTT11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTCATGCTTCTGATAGCATA11.3157894736842104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGCA50.0245.000024
GAGCAAA50.0245.000026
GGGGGGA50.0245.000021
GGGGGAG50.0245.000022
GCAAAAG50.0245.000028
AGCAAAA50.0245.000027
GGGGAGC50.0245.000023
GGAATCC50.0245.00002245
GGAGCAA50.0245.000025
CAAAAGC50.0245.000029
Produced by FastQC (version 0.11.2)