FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated f4.341000000085cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated f4.341000000085cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279446
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG222137.948941834916227No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA145885.220328793398367No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA111213.9796597553731314No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT99893.5745725471110696No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC80142.8678170379966077No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG71352.5532661050793353No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT66592.382929081110483No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG43421.5537885673797442No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA37241.3326367169327884No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA28581.0227378455944978No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG24850.8892594633668044No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG23140.8280669610586661No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA20570.7360992821511133No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19150.6852844556730101No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC15790.5650465564008789No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA14200.508148264781031No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT8150.29164847591305654TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA6110.2186468942121197No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5940.21256342907037495No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4990.1785675944547426No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA4360.15602298834121797No Hit
GGGGGGAGCGAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA3830.13705689113460204No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3450.12345855728834909No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3370.12059575016282215No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT2890.10341890740966055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGT151.5181424E-5245.09
CGTACTT151.5181424E-5245.0245
CGGGTTA62450.0244.215381
GGGGGGA52700.0243.140431
GGGTTAT62800.0243.049362
TATTAGT63100.0241.699686
GGTTATT63200.0241.511083
TTATTAG63300.0241.129555
GTTATTA63300.0241.129554
ATTAGTA63200.0241.123437
TAGTAGA63500.0239.598439
GGGGGAG55500.0230.432452
GGAGCGA40200.0224.583335
GCGAAAG40050.0224.506878
GAGCGAA40350.0223.748446
AGCGAAA40400.0223.471537
GGGGAGC57200.0223.369753
CGAAAGC40150.0223.032389
GGGAGCG41000.0220.201224
TTAGTAG71550.0212.983928
Produced by FastQC (version 0.11.2)