FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated f3.34100000008546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated f3.34100000008546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209397
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4949423.636441782833565TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC36581.74692092054805No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA36171.7273408883603871No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG31831.5200790842275678No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC20550.981389418186507No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG20060.9579888919134466No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG17630.8419413840694948No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA13510.6451859386715186No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC11510.5496735865365788No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG9990.4770841989140246No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG8220.3925557672746028No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG7590.36246937635209675No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA6830.3261746825408196No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC6750.322354188455422No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA5530.2640916536531087No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT4860.23209501568790383No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3300.15759538102265075No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3120.14899926933050617No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA2880.13753778707431338No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT2680.1279865518608194TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA2590.1236884960147471No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT2520.12034556369002422TruSeq Adapter, Index 11 (97% over 37bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA2140.10219821678438565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA14550.0245.01
GGGTTAT14650.0243.327642
GGGGGGA16850.0239.183981
GGTTATT15200.0234.523033
TATTAGT15450.0230.728156
ATTAGTA15550.0229.244357
TTATTAG15550.0229.244355
GTTATTA16300.0218.69634
GAGCGAA12200.0217.889346
GGAGCGA12150.0217.777795
GCGAAAG12200.0216.885248
GGGGGAG18950.0212.67812
AGCGAAA12500.0211.680017
CGAAAGC12700.0208.346459
TAGTAGA17000.0208.249989
TTAGTAG18250.0194.657538
GGGGAGC21300.0189.213623
GGGAGCG14450.0182.266454
CGAGAGG3300.0152.19698245
GAGCAAA9800.0142.56
Produced by FastQC (version 0.11.2)