FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated e5.34100000008638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated e5.34100000008638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78278
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT6877787.86249009938936TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT6800.8686987403868264TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATTTCGTAT3270.4177418942742533TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC1860.23761465545874957TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT1510.19290222029178056TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAATCTCGTATG1490.1903472239965252TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTAGAAATCTCGTAT1230.15713227215820535TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAAGAAATCTCGTAT1080.13796979994379008TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG1030.13158230920565164TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGGAATCTCGTAT970.12391732031988555TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAACCTCGTAT910.11625233143411944TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCGAAATCTCGTAT840.10730984440072563TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGCAT800.10219985181021488TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA400.0245.000021
GATCGGA79100.0232.300891
TCGGAAG79200.0231.852923
ATCGGAA79500.0231.286182
CGGAAGA79300.0231.251594
GAAGAGC79800.0229.495626
GGAAGAG80100.0228.941945
AAGAGCA80100.0228.33027
AGAGCAC80250.0226.83498
GAGCACA80550.0226.142149
CGGGTTA400.0214.375021
GGGTTAT400.0214.375022
GGTTATT451.8189894E-12190.555543
GGGGAGC600.0163.333343
GGAGCGA452.8012437E-10163.333335
GGGAGCG505.838956E-10147.04
GAGCGAA505.838956E-10147.06
GCGAAAG551.1350494E-9133.636378
ATTAGTA652.3646862E-11131.923087
GGGGGAG750.0130.666662
Produced by FastQC (version 0.11.2)