FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated e3.34100000008539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated e3.34100000008539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102699
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT9171789.30661447531135TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT5150.5014654475700834TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT4370.4255153409478184TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT3840.37390821721730494TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC3070.29893182991070993TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATTTCGTAT3060.2979581105950399TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTCATTATCTCGTAT2360.22979775849813533TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG1800.1752694768206117TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTACCTCGTAT1680.1635848450325709TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTCATTATCTCGTAT1490.1450841780348397TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCCATTATCTCGTAT1480.1441104587191696TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCACTATCTCGTAT1350.13145210761545878TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGCAT1290.12560979172143838TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATATCTCGTATG1160.11295144061772752TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTTATTATCTCGTAT1030.10029308951401668TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAG400.0245.000022
TCGGAAG104650.0235.869573
GATCGGA104800.0235.298171
CGGAAGA104800.0235.181294
ATCGGAA105000.0234.966672
GAAGAGC105450.0233.266976
GGAAGAG105900.0232.738455
AGAGCAC105950.0231.588038
GAGCACA105950.0231.47249
AAGAGCA106600.0230.635567
GGGGAGC450.0217.777793
GGGAGCG354.9112714E-11210.04
CGGGTTA254.7290632E-7196.01
TCGGGAA150.004960414163.333333
GGGGGGA600.0163.333331
GGAGCGA505.857146E-10147.05
GCGAAAG551.1386874E-9133.636378
GGGTTAT404.908592E-6122.500012
GTTATTA404.908592E-6122.500014
TTAGTAG501.4878611E-598.08
Produced by FastQC (version 0.11.2)