FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated d7.3410000000872a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated d7.3410000000872a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT189.836065573770492TruSeq Adapter, Index 19 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG105.46448087431694No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA52.73224043715847No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT42.185792349726776No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT31.639344262295082No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC31.639344262295082No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG31.639344262295082No Hit
TTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGG21.092896174863388No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA21.092896174863388No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGCACCGCTCATTATCTCGTATG21.092896174863388Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA21.092896174863388No Hit
GGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACT21.092896174863388No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA21.092896174863388No Hit
CGGGTTATTAGTAGAAACAAGGGGATTCATGCCAAAATTAGGAAAAACAG21.092896174863388No Hit
GGGGGGAGCGAAAGCAGGAGATATAAACAGCCGAAGACAAGGAGCGGCTC10.546448087431694No Hit
AGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAGTGAAAG10.546448087431694No Hit
ACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGC10.546448087431694No Hit
AACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTCAGGATA10.546448087431694No Hit
ATTTTCCAAATTAGATGAAAAAACACATCAATAGCTCTAAGAAGCTCAAT10.546448087431694No Hit
TCTCGAACATGTACCCTTTCCCTAGTCTTGCCATTTTGTTTGAGAACATT10.546448087431694No Hit
TTTATCTACCCTTGTTTGTTGAACAACTTCCATTGTTTCAAGGCATGAAT10.546448087431694No Hit
AGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATT10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG10.546448087431694No Hit
TGGAAAATGTCCAAAATATGTAAAAAGCACAAAATTGAGACTGGCCACAG10.546448087431694No Hit
AAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAG10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGAAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGCGACAAAAACATAATGGACTCCAACACCATG10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAATCAATTAGTAACTTGTTG10.546448087431694No Hit
AAAGAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACATT10.546448087431694No Hit
ATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTAT10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA10.546448087431694No Hit
AGAAGAAATAAGGAGAGTTTGGCGCCAAGCAAACAATGGCGAAGATGCAA10.546448087431694No Hit
GGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT10.546448087431694No Hit
ATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAACAT10.546448087431694No Hit
GGAGACAATCCACGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATC10.546448087431694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTACGAGATTCCATATCGTATG10.546448087431694Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGGGGAGCAAAAGCAGGCAGACCATTTGACGGGAAGGCAAACCGACACT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGTCAAATATAATCAATATGGAGTGAATAAAAGA10.546448087431694No Hit
GTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACAC10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGTCTCATATTGCCTTAGTGCACCTTTGGA10.546448087431694No Hit
ATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTT10.546448087431694No Hit
AAATTTCTCCAACTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATC10.546448087431694No Hit
TCCCCATTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCC10.546448087431694No Hit
TTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCA10.546448087431694No Hit
ATCCGCAGGCCAGATCAGTGTGCAGCCTACATTCTCAGTGCAGCGGAATC10.546448087431694No Hit
AAAACAGGTGAGTATGTTGCCATTAAAACTTGACAGTAAGGGGCACCTGG10.546448087431694No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT10.546448087431694No Hit
AAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGAAGT10.546448087431694No Hit
ATTAGGGTTCGATATGGGCTCCTGTCCTTAATGGTTCCATTGGAATGTTT10.546448087431694No Hit
GTATGAATTTCCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTT10.546448087431694No Hit
AGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAATGGAAT10.546448087431694No Hit
TGGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGG10.546448087431694No Hit
ATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCAAACAA10.546448087431694No Hit
CCCAAGAAATACTCTCATTTTCCTACCTAGAGCTGAAAGTCTGCCTGCCA10.546448087431694No Hit
AAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGAC10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGAAAGTATTTCCATTGGAAAATAAGACAGTCTT10.546448087431694No Hit
ATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCAG10.546448087431694No Hit
AAATTAGCGGGCAATTCCTCTCTCTGCCCTGTTAGTGGATGGGCTATATA10.546448087431694No Hit
GAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAA10.546448087431694No Hit
AATAGCAATAAGACCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATT10.546448087431694No Hit
GGTGCTGCGGTGAAAGGAGTTGGAACAATAGCAATGGAGTTAATCAGAAT10.546448087431694No Hit
TGATCTCGGCGGAGCCGATCAAGGAATGGGGCATCACCCAATCCATTGTC10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10.546448087431694No Hit
CATTCTTATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTGTTCC10.546448087431694No Hit
GGATGTCCGTCCTTCATTGTTCCCGCTGAATGCTGCCATAACGGTTGCTC10.546448087431694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGAGCTATCTCGTAT10.546448087431694TruSeq Adapter, Index 12 (97% over 37bp)
ATCAAAAGCAGAAAGCACCATCCTCTCTATTGTTATGCTATTCTGGATTA10.546448087431694No Hit
AAGATGCAAAAGTGCTTTTCCAGAACTGGGGAATTGAATCCATCGACAAT10.546448087431694No Hit
ATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGC10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGACAATGGGAGAGAGGGAGGCACGCTTCCTGCT10.546448087431694No Hit
ATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCT10.546448087431694No Hit
AGCAACACCAACTTTGCTGCTGGACAGTCAGTGGTTTCCGTGAAATTAGC10.546448087431694No Hit
ACAAAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAGATATGCA10.546448087431694No Hit
AGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGTTTCCC10.546448087431694No Hit
CAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACA10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGAGACAAAAACATAATGGACTCCAACACCATG10.546448087431694No Hit
AAGGACAAGGGTTGCTTATGAAAGAATGTGCAATATCCTCAAAGGAAAAT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGAGAGGCAGAGAGAGAAGCAGGCTCCCCACTG10.546448087431694No Hit
AGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGTATCAGAT10.546448087431694No Hit
GGGAGCGAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTAT10.546448087431694No Hit
ATCCGACATGCGAACAGAAGTTATAAGAATGATGGAAAGTGCAAAGCCAG10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA10.546448087431694No Hit
TTTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAGA10.546448087431694No Hit
TGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAG10.546448087431694No Hit
TTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAGT10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGTAGGCAGAGGGAAAAGCAGGCTCC10.546448087431694No Hit
ACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTT10.546448087431694No Hit
AAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACC10.546448087431694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT10.546448087431694TruSeq Adapter, Index 7 (97% over 38bp)
CTCCTGCATTGAATTTAGAGAAAACTCTGCAGCCAGTATTGTCAGGACTG10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA10.546448087431694No Hit
GCTGAATGCTGCCATAACGGTTGCTCTTTCAAAAGGGAGATTCCGCTGCA10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGGGAGTGGAAGGCAGAGTGAGAAGAAGCCTCC10.546448087431694No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT10.546448087431694No Hit
AAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAA10.546448087431694No Hit
ACAGAAGTTATAAGAATGATGGAAAGTGCAAAGCCAGAAGATTTGTCCTT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGTGGGGGTGCTCTGGGGAAATGAAAGAGGGAGG10.546448087431694No Hit
CCAGTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATG10.546448087431694No Hit
TCTCTATTGTTATGCTATTCTGGATTAGTCGTCCCTCATAATCACTGAGT10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGCAGAGCGGCAGGCAGAGGGAGAAGCAGGCTCC10.546448087431694No Hit
GGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTGGA10.546448087431694No Hit
GCTCTATTGGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCTATAT10.546448087431694No Hit
ATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGGCCT10.546448087431694No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT10.546448087431694No Hit
GGATTCCTCTTTCAAGATCCATTCCACGATTTGTTTCCCAACAAGAGTGG10.546448087431694No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT10.546448087431694No Hit
AAACAATATATTGAGAACACAAGAGCCTGAATGTGCATGTGTAAATGGTT10.546448087431694No Hit
GGACAAACCCTCTGGAGCAAAACAAACGATATTGACTGTGAATGTGAGAG10.546448087431694No Hit
GTTCACTCGATCCAGCCATCTGTTCCATAGTCTTTGCCGTAGTGCTAGCC10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTG10.546448087431694No Hit
CTACTGCAAGCCCATACACACAAGCAGGCAGGCAGGATTTATGTGCAACT10.546448087431694No Hit
CGAATGTTATTTTGTCTCCCGGCTCTACTAGTGTCCAGTAATAGTTCATT10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGAAATTAAAAGGTGCTCTTCAGGAGACTT10.546448087431694No Hit
GAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAAT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA10.546448087431694No Hit
GAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCATTGGT10.546448087431694No Hit
AGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTGGAATCAACAA10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGAAATAGGGACCAGTGTATTGAAAACCTGGGAC10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGCTCCCTGATGAATGGGGACTGGATGTGGGGCT10.546448087431694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAATCCATCTCGTAT10.546448087431694TruSeq Adapter, Index 19 (97% over 38bp)
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG10.546448087431694No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTAATTATCTCGTAT10.546448087431694TruSeq Adapter, Index 2 (97% over 37bp)
ACTCCTGGTCCTTATGGCCCAGTATCTGCTTCTCAGTTCCAGGGTATTGG10.546448087431694No Hit
ATCCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAA10.546448087431694No Hit
GTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAAT10.546448087431694No Hit
TTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCT10.546448087431694No Hit
AGCCTCACCCTGCTCAGGACTGTCACTCATTAGCCATAATGTTGTATTCC10.546448087431694No Hit
ATTCGGGTCACCAGATTCTACAATTATTGATTCACCCCGTTCGTCGATGA10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT10.546448087431694No Hit
CCTCTCGTCATTGCAGCAAATATCATTGGGATCTTGCACCTGATATTGTG10.546448087431694No Hit
AGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGC10.546448087431694No Hit
TGGTCAAATAGAACACGTTAATAGCTGGAAGACAAATTTTTTAAAAAAGC10.546448087431694No Hit
GTTTGATCATTCTGATTAACTCCATTGCTATTGTTCCAACTCCTTTCACC10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC10.546448087431694No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers