FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated d5.3410000000862b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated d5.3410000000862b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229584
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA71143.0986479894069276No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC53942.3494668618022163No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG47132.0528434037215137No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG41581.8111018189420864No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC28671.2487804028155272No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG25221.098508606871559No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA22920.9983274095755801No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG22640.9861314377308522No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT21940.9556415081190327TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA20730.9029374869328872No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT14170.6172032894278346No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG13400.5836643668548331No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA13350.5814865147397031No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13160.5732106767022093No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA11550.503083838595024No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG9940.4329570004878389No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA8550.3724127116872256No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8100.3528120426510558No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC7270.31665969753989825No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA4800.20907380305247752No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4730.20602481009129558No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4120.17945501428670987No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA3320.1446093804446303No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA2430.10584361279531675No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACC100.0014754345244.99998245
CGGGTTA29250.0244.162381
GGGGGGA23850.0243.459121
GGGTTAT29750.0240.058822
GGTTATT30150.0236.873963
TATTAGT30350.0234.90946
ATTAGTA30350.0234.505777
TTATTAG30500.0233.75415
GTTATTA30950.0230.35544
TAGTAGA32100.0220.576319
GGGGGAG26350.0219.895652
GGAGCGA17800.0219.536515
CGAAAGC17900.0218.310069
GCGAAAG17900.0218.310068
GAGCGAA17900.0218.310066
AGCGAAA17900.0218.310067
GGGGAGC28050.0204.821753
TTAGTAG34750.0204.812948
GGGAGCG19500.0200.397434
CGAGAGG3450.0166.88406245
Produced by FastQC (version 0.11.2)