FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated d4.341000000085a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated d4.341000000085a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198537
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2240211.28353908843188TruSeq Adapter, Index 7 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG156777.896261150314551No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA105635.320418863990088No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA41952.1129562751527424No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG41892.1099341684421544No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA36541.840462986748062No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC30501.5362375778822084No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT30491.5357338934304439No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG28721.4465817454680991No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT23471.1821474082916534No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG16490.8305756609599217No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC14270.7187577126681676No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA13660.6880329611105235No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG13020.6557971561975853No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT11700.5893108085646503No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA9380.47245601575524965No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC7270.3661785964329067No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA6710.33797226713408585No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5800.2921369820235019No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA4770.24025748349174209No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT4530.22816905664939027TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3570.17981534927998308No Hit
GGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACT3500.17628955811763045No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3350.1687342913411606No Hit
GGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAAC2770.1395205931388104No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA2490.12541742848939996No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG2450.12340269068234133No Hit
AATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACG2280.11484005500234212No Hit
ATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGG2010.10124057480469636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA32950.0243.884671
CGGGTTA32050.0243.471131
GGGTTAT32400.0240.841052
GGTTATT32750.0239.015263
GTTATTA32950.0237.564484
TTATTAG33100.0236.858025
TATTAGT33400.0234.730556
ATTAGTA33450.0234.379677
GGGGGAG34450.0232.910022
TAGTAGA33900.0230.907079
GGGGAGC36150.0221.957143
GAGCGAA24050.0220.550956
GGAGCGA24100.0220.093375
GCGAAAG24100.0220.093378
AGCGAAA24400.0217.387287
CGAAAGC24500.0216.09
GGGAGCG24750.0214.313134
TTAGTAG37700.0207.632618
GAGCAAA14050.0194.430626
CGAGAGG2400.0183.75245
Produced by FastQC (version 0.11.2)