##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE52Y l01n01 flu mrt-pcr updated d3.3410000000852c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 377415 Sequences flagged as poor quality 0 Sequence length 251 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92896943682683 33.0 32.0 34.0 32.0 34.0 2 33.13508472106302 33.0 33.0 34.0 32.0 35.0 3 33.20391876316521 33.0 33.0 34.0 32.0 35.0 4 33.28508670826544 34.0 33.0 34.0 32.0 35.0 5 32.945232701402965 33.0 33.0 34.0 32.0 34.0 6 34.08639826186029 34.0 33.0 37.0 32.0 37.0 7 34.1834134838308 34.0 33.0 37.0 32.0 37.0 8 34.934345481764105 35.0 34.0 37.0 33.0 37.0 9 34.83644529231748 35.0 33.0 37.0 32.0 37.0 10-14 36.331500867745056 37.4 36.0 37.4 34.6 37.4 15-19 37.370524753918104 38.0 38.0 38.0 37.0 38.0 20-24 37.4405850323914 38.4 38.0 38.4 36.8 38.4 25-29 37.66881867440351 39.0 38.0 39.0 36.6 39.0 30-34 37.51825285163547 39.0 38.0 39.0 36.2 39.0 35-39 37.125234556125214 38.0 38.0 39.0 35.2 39.0 40-44 37.411739332035026 39.0 38.0 39.0 35.8 39.0 45-49 37.51236490335572 39.0 38.0 39.0 36.2 39.0 50-54 37.58566670641072 39.0 38.0 39.0 36.4 39.0 55-59 37.360090086509544 38.8 38.0 39.0 36.0 39.0 60-64 37.49923082018468 39.0 38.0 39.0 36.2 39.0 65-69 37.396854920975585 39.0 38.0 39.0 36.0 39.0 70-74 36.80594517970934 38.2 37.8 39.0 34.0 39.0 75-79 34.70950757124121 38.4 36.0 39.0 18.6 39.0 80-84 34.543859677013366 38.8 36.2 39.0 18.0 39.0 85-89 34.53924088867692 38.6 36.0 39.0 18.0 39.0 90-94 34.467253818740645 38.0 36.0 39.0 17.4 39.0 95-99 34.49990328948239 38.0 36.0 39.0 17.0 39.0 100-104 34.245033716201 38.0 35.0 39.0 17.0 39.0 105-109 34.30104685823298 38.0 35.6 39.0 17.0 39.0 110-114 34.2433538677583 38.0 35.8 39.0 17.0 39.0 115-119 34.07444007259913 38.0 35.0 39.0 16.0 39.0 120-124 33.599015937363376 38.0 33.4 39.0 16.0 39.0 125-129 33.93765960547408 38.0 35.0 39.0 16.0 39.0 130-134 33.782698090960885 38.0 34.6 39.0 16.0 39.0 135-139 33.656980777128624 38.0 34.2 39.0 16.0 39.0 140-144 33.39578236159135 38.0 33.0 39.0 15.2 39.0 145-149 33.42042102195196 38.0 33.4 39.0 15.0 39.0 150-154 33.17371328643535 38.0 32.6 39.0 15.0 39.0 155-159 33.00521653882331 37.0 31.8 39.0 15.0 39.0 160-164 33.02054290370017 37.0 33.0 39.0 15.0 39.0 165-169 32.88424519428216 37.0 32.2 39.0 15.0 39.0 170-174 32.62044327861903 37.0 30.6 39.0 15.0 39.0 175-179 32.265080084257384 37.0 27.2 39.0 15.0 39.0 180-184 31.992557264549635 37.0 26.4 38.0 14.8 39.0 185-189 32.100948292993124 37.0 26.4 38.0 15.0 39.0 190-194 31.89588278155346 37.0 26.0 38.0 14.2 39.0 195-199 31.517735649086553 37.0 25.4 38.0 14.0 39.0 200-204 31.295575957500365 37.0 24.8 38.0 14.0 39.0 205-209 30.87010320204549 37.0 24.0 38.0 14.0 39.0 210-214 30.502947683584388 37.0 22.4 38.0 14.0 39.0 215-219 30.026423433090894 37.0 17.6 37.4 14.0 38.2 220-224 29.649188294052966 37.0 15.2 37.0 14.0 38.0 225-229 29.092459494190745 36.0 15.0 37.0 13.8 38.0 230-234 28.64047268921479 35.8 15.0 37.0 13.2 38.0 235-239 27.955874567783475 33.4 15.0 37.0 13.0 38.0 240-244 26.898107918339225 30.4 14.4 37.0 13.0 37.4 245-249 26.048174025939613 26.2 14.0 37.0 13.0 37.0 250-251 24.43013923664931 25.0 14.0 35.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.051081038529829925 1101 2 -0.003793298826487046 1101 3 0.05291867486597113 1101 4 0.044972978679709286 1101 5 0.09260575773486579 1101 6 0.05442930033047588 1101 7 0.0480673246959995 1101 8 0.025365300252452982 1101 9 0.059133035325224625 1101 10-14 0.12527649553245368 1101 15-19 0.09688168796324703 1101 20-24 0.03815621071414199 1101 25-29 0.16228713042486476 1101 30-34 0.1290580913620616 1101 35-39 0.1423124289271982 1101 40-44 0.13989575699419277 1101 45-49 0.16935356288604453 1101 50-54 0.20396193525957784 1101 55-59 0.16262478273436187 1101 60-64 0.18709500016277048 1101 65-69 0.20761155399521414 1101 70-74 0.210287003523554 1101 75-79 0.2199677227437462 1101 80-84 0.006550791863553229 1101 85-89 0.1653637331387543 1101 90-94 0.0940761194380002 1101 95-99 0.04656322490441056 1101 100-104 0.06247938713210033 1101 105-109 0.19314131901575138 1101 110-114 0.0864500994880757 1101 115-119 0.03804957005453247 1101 120-124 0.5710273871915916 1101 125-129 0.09230244682191824 1101 130-134 0.09171138818224023 1101 135-139 0.12626272677708528 1101 140-144 0.2338353721262152 1101 145-149 0.1645626663797941 1101 150-154 0.36872120497411487 1101 155-159 0.3783469093050229 1101 160-164 0.15540576269503958 1101 165-169 0.13964696237848528 1101 170-174 0.15807228048214483 1101 175-179 0.26725551679844983 1101 180-184 0.5071299388244022 1101 185-189 0.18151332616273663 1101 190-194 0.14155130272103378 1101 195-199 0.3397837665467236 1101 200-204 0.31868552189663646 1101 205-209 0.32605687906449177 1101 210-214 0.23988412349254773 1101 215-219 0.4002222107258149 1101 220-224 0.5178511265289032 1101 225-229 0.6283330346138776 1101 230-234 0.36652223851587706 1101 235-239 0.3460092429052999 1101 240-244 0.7461322440569624 1101 245-249 0.3992084804435798 1101 250-251 0.4364884116260619 1102 1 -0.08834624423022319 1102 2 0.051829581259418944 1102 3 0.006055770370728908 1102 4 -0.08072970613151398 1102 5 0.10629935157818693 1102 6 -0.028269181848770586 1102 7 0.022658543804283227 1102 8 -0.021120251448117244 1102 9 0.010714359165085341 1102 10-14 0.10914218381316942 1102 15-19 -0.04099159481200587 1102 20-24 -0.09072550966959625 1102 25-29 -0.00359974567619048 1102 30-34 0.12604185656667966 1102 35-39 0.0862300409524579 1102 40-44 0.02109280631096766 1102 45-49 0.11216240458546878 1102 50-54 0.07697495716544012 1102 55-59 0.1685477121995831 1102 60-64 0.02862407681281809 1102 65-69 0.21638446739597583 1102 70-74 0.22768102668654677 1102 75-79 0.09823651437384484 1102 80-84 0.1331000407158669 1102 85-89 0.1971361915598706 1102 90-94 0.26103877444913337 1102 95-99 0.26740495882972226 1102 100-104 0.2845694041021929 1102 105-109 0.22213107011384636 1102 110-114 0.2744696873325694 1102 115-119 0.21235055095616673 1102 120-124 0.6881934892665953 1102 125-129 0.24271807397148848 1102 130-134 0.26051185911853025 1102 135-139 0.26090414458857936 1102 140-144 0.24280429335004783 1102 145-149 0.18445519630386542 1102 150-154 0.4055016740559836 1102 155-159 0.45540234997761786 1102 160-164 0.3726160689545708 1102 165-169 0.2755487385241935 1102 170-174 0.42564624702065856 1102 175-179 0.46210648400756327 1102 180-184 0.7150709510355391 1102 185-189 0.23955342043625194 1102 190-194 0.21952985614448295 1102 195-199 0.43830648426311214 1102 200-204 0.43467557703326776 1102 205-209 0.3252029835976842 1102 210-214 0.4597281366287369 1102 215-219 0.46650002432216553 1102 220-224 0.6015352542078389 1102 225-229 0.7444910656516832 1102 230-234 0.4762829537180231 1102 235-239 0.3871728264199419 1102 240-244 0.8089578186574542 1102 245-249 0.49253968622592126 1102 250-251 0.4277995939909651 1103 1 -0.004493920619331959 1103 2 -0.037163200542693176 1103 3 -0.13478232600566287 1103 4 0.09107919730854519 1103 5 0.025903524558820834 1103 6 0.06850311837020939 1103 7 0.06848300201257729 1103 8 0.11273868848845581 1103 9 0.1564399993479526 1103 10-14 0.067135772380162 1103 15-19 0.08570378528524003 1103 20-24 0.09370158055574507 1103 25-29 0.17602431896744264 1103 30-34 0.20499809917667022 1103 35-39 0.20443710206596677 1103 40-44 0.1892044642768127 1103 45-49 0.12291372038055925 1103 50-54 0.17873425950591582 1103 55-59 0.09772592186454432 1103 60-64 0.13605655827642948 1103 65-69 0.2909363714037738 1103 70-74 0.30786747901272093 1103 75-79 3.4447519084324085E-5 1103 80-84 0.03181754068385345 1103 85-89 0.0660940989227683 1103 90-94 0.0434013576334209 1103 95-99 -0.033559103850357985 1103 100-104 0.014233166699881394 1103 105-109 -0.5928645118880382 1103 110-114 -0.02217903699715862 1103 115-119 -0.32549525877538343 1103 120-124 0.31329091999793945 1103 125-129 0.11316145316378368 1103 130-134 -0.04960236854283551 1103 135-139 0.005716304968309771 1103 140-144 0.23539528917109465 1103 145-149 0.1620139001870129 1103 150-154 0.21968600915793246 1103 155-159 0.32623395808624167 1103 160-164 0.19100433201967348 1103 165-169 0.030297979781032325 1103 170-174 0.2412979124138559 1103 175-179 -0.128341249153884 1103 180-184 0.31021520827827587 1103 185-189 0.29295271782514476 1103 190-194 0.35442136974636895 1103 195-199 0.4486894201189031 1103 200-204 0.5802556580512466 1103 205-209 0.44891312023548124 1103 210-214 0.5813105909867637 1103 215-219 0.7073921184532708 1103 220-224 0.7393503300394677 1103 225-229 0.6973751433572275 1103 230-234 0.561590015258389 1103 235-239 0.5903616819663462 1103 240-244 1.019809019627278 1103 245-249 0.6992144916846037 1103 250-251 0.576415074485606 2112 1 -0.18697518985396044 2112 2 0.025360659455898826 2112 3 -0.1403999560653162 2112 4 0.07054093706767617 2112 5 -0.08100899695516262 2112 6 0.10264664305984184 2112 7 0.03934607814490221 2112 8 0.017730573423008877 2112 9 0.14250362743268852 2112 10-14 -3.229218301115111E-4 2112 15-19 -0.08424615937023106 2112 20-24 -0.1039864255694738 2112 25-29 -0.061433906040612385 2112 30-34 -0.1588242779296749 2112 35-39 -0.19304573473057474 2112 40-44 -0.1525661555606348 2112 45-49 0.03739415358215581 2112 50-54 -0.013611026398187676 2112 55-59 0.005054966974071817 2112 60-64 -0.05235649322276714 2112 65-69 0.07711175492324429 2112 70-74 0.05328646591984665 2112 75-79 0.1545723793184024 2112 80-84 0.1031854357361155 2112 85-89 0.24137055499584648 2112 90-94 0.43811771423563073 2112 95-99 0.278094705964925 2112 100-104 0.49192110884298046 2112 105-109 -0.26764873219126883 2112 110-114 0.15747358275071832 2112 115-119 0.12347900121809374 2112 120-124 0.590416565950747 2112 125-129 0.33694488648694687 2112 130-134 0.22741301489134003 2112 135-139 0.3832745766833128 2112 140-144 0.5166685759965333 2112 145-149 0.28394441134016546 2112 150-154 0.3026805216248505 2112 155-159 0.41043538659368295 2112 160-164 0.4788131790405643 2112 165-169 0.3102373460594734 2112 170-174 0.3852261159452439 2112 175-179 -0.02505448769415608 2112 180-184 0.4311814022592664 2112 185-189 0.21824932826574894 2112 190-194 0.15245733196280042 2112 195-199 0.3169269899927123 2112 200-204 0.23108326676219448 2112 205-209 0.44931055583214885 2112 210-214 0.15516962984736793 2112 215-219 0.2335451295584292 2112 220-224 0.33480546684390333 2112 225-229 0.11976014082993203 2112 230-234 -0.20074200501968065 2112 235-239 0.049398516472329845 2112 240-244 0.36763714659339897 2112 245-249 0.17102759564915004 2112 250-251 -7.66555821439141E-4 1113 1 0.03161591301848432 1113 2 0.019319988143159605 1113 3 0.06663489583448978 1113 4 -0.0202438102901894 1113 5 0.10426736957997917 1113 6 0.08489356952689775 1113 7 0.09066849394761789 1113 8 0.15235676850937807 1113 9 0.11382208387495751 1113 10-14 0.058620627641253975 1113 15-19 0.062001287953869166 1113 20-24 0.055697968939512066 1113 25-29 0.06271808681983515 1113 30-34 0.04500560931323605 1113 35-39 0.12507246805854777 1113 40-44 0.05577528528662867 1113 45-49 0.09021509203527955 1113 50-54 0.08197015795874307 1113 55-59 -0.020391016868785528 1113 60-64 0.10039902050424843 1113 65-69 0.11032602513583356 1113 70-74 0.2563656626566555 1113 75-79 0.013560709791619274 1113 80-84 -0.005027484694501538 1113 85-89 0.06855008234983728 1113 90-94 0.13750083740879404 1113 95-99 -0.022605063354689037 1113 100-104 0.12421953875542613 1113 105-109 -0.07994117013041091 1113 110-114 0.02299443450019112 1113 115-119 0.013364634985720159 1113 120-124 0.6076024134603131 1113 125-129 0.14773170880376796 1113 130-134 -0.05579633258700056 1113 135-139 0.033180621271633015 1113 140-144 0.178520349821774 1113 145-149 -0.353151090762843 1113 150-154 0.1725753021502996 1113 155-159 0.2451871378696282 1113 160-164 -0.031332417441326754 1113 165-169 0.04393457355438102 1113 170-174 0.1577235937055974 1113 175-179 -0.1566958119837807 1113 180-184 0.23675531367334557 1113 185-189 -0.02047776911016541 1113 190-194 0.044703337402154375 1113 195-199 0.1317852840588607 1113 200-204 0.05770668394433898 1113 205-209 0.2995016955456933 1113 210-214 0.0670337369745404 1113 215-219 0.14986510516708051 1113 220-224 0.06454979167238051 1113 225-229 0.10186097324841015 1113 230-234 0.37274467766087227 1113 235-239 0.5867388849045803 1113 240-244 0.5492141577420675 1113 245-249 0.29622098499931937 1113 250-251 0.3796094140366044 2113 1 0.0722160233788216 2113 2 0.08176311612991327 2113 3 -0.11762730366784524 2113 4 0.06307407953687516 2113 5 0.16154230216647392 2113 6 0.13510231714530363 2113 7 0.13811510973747687 2113 8 0.11025950565396414 2113 9 0.14290307378635703 2113 10-14 -0.06634787801731079 2113 15-19 0.06239275480932349 2113 20-24 0.021458570299230928 2113 25-29 0.16962102178012373 2113 30-34 0.07015110796497481 2113 35-39 0.14890655274448505 2113 40-44 0.14454372639481505 2113 45-49 0.11432299529299428 2113 50-54 0.12507211633415238 2113 55-59 0.07641103208540301 2113 60-64 0.10890426262025699 2113 65-69 0.13749299199650267 2113 70-74 0.13348192623045207 2113 75-79 0.1558387137270998 2113 80-84 0.14371992548154822 2113 85-89 0.04781316681714287 2113 90-94 0.18205018201414447 2113 95-99 0.16820296171584914 2113 100-104 0.08010518045913528 2113 105-109 0.21112693052653952 2113 110-114 0.1587803354465791 2113 115-119 0.18407874965635074 2113 120-124 0.6219594603842822 2113 125-129 0.1589722214587681 2113 130-134 0.020722735805172476 2113 135-139 0.14473805549791052 2113 140-144 0.16995493008809603 2113 145-149 0.03685963293026617 2113 150-154 0.23574889063118576 2113 155-159 0.23491592213598977 2113 160-164 0.14852598816415252 2113 165-169 0.020198980263444355 2113 170-174 0.23679469283208476 2113 175-179 0.1950725562449378 2113 180-184 0.55345345732918 2113 185-189 0.3079385600551561 2113 190-194 0.15210872423596555 2113 195-199 0.18850903964194643 2113 200-204 0.17851981057761535 2113 205-209 0.05075782522950334 2113 210-214 -0.20124703638072816 2113 215-219 -0.03003536230265169 2113 220-224 0.07227563993732034 2113 225-229 -0.5966400002130499 2113 230-234 0.247624790936392 2113 235-239 0.3068134301887575 2113 240-244 0.35626097759443454 2113 245-249 0.19889316274192836 2113 250-251 -0.21673151611363295 1112 1 -0.0065217886638109235 1112 2 -0.013708014129200308 1112 3 0.0453470732775898 1112 4 -0.09390371931286978 1112 5 -0.03518730555136074 1112 6 -0.055591546102206735 1112 7 0.06302801608861586 1112 8 -0.013761850327156822 1112 9 -0.009573684062374355 1112 10-14 0.05945451731570728 1112 15-19 0.05351435609563282 1112 20-24 0.06999787066409624 1112 25-29 0.11862166081044023 1112 30-34 0.1568726762690673 1112 35-39 0.15263436705464528 1112 40-44 0.17628333184512002 1112 45-49 0.1122727386501623 1112 50-54 -0.09217345683481426 1112 55-59 -0.0012499107935397546 1112 60-64 0.028184838159823755 1112 65-69 -0.02571720775200248 1112 70-74 0.022436802444097736 1112 75-79 0.19159885060327042 1112 80-84 0.08625337150410672 1112 85-89 0.03886344407384712 1112 90-94 0.2544537605839565 1112 95-99 0.06438671385453887 1112 100-104 0.2813411887871524 1112 105-109 0.05087770870055408 1112 110-114 0.1392187927037014 1112 115-119 0.004884752418618632 1112 120-124 0.40192441678592417 1112 125-129 -0.030318042605053108 1112 130-134 0.08373277476540153 1112 135-139 0.22051117911935592 1112 140-144 0.29149643613827436 1112 145-149 0.18119782426830966 1112 150-154 0.13852784798106654 1112 155-159 0.23241154168620426 1112 160-164 0.062403665391997265 1112 165-169 -0.06701587822225719 1112 170-174 0.02104053398151251 1112 175-179 0.02308682105086035 1112 180-184 0.31656780252923866 1112 185-189 -0.17096292620569287 1112 190-194 0.16964144877265852 1112 195-199 0.14291030945704009 1112 200-204 0.07255477172045133 1112 205-209 0.11592758054272778 1112 210-214 0.29400867881788884 1112 215-219 0.14968037227301423 1112 220-224 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-0.011045495479223177 2110 45-49 0.04276696404436109 2110 50-54 -0.05391120008020067 2110 55-59 -0.16115771046656846 2110 60-64 -0.10295013116533624 2110 65-69 -0.09886133558437393 2110 70-74 -0.1281191242175268 2110 75-79 0.11565223714149653 2110 80-84 0.09029515609460503 2110 85-89 0.19618585516953146 2110 90-94 0.1224279901970533 2110 95-99 0.03197640921551681 2110 100-104 -1.2878894790279887 2110 105-109 -0.03492616564682294 2110 110-114 0.0791382822179969 2110 115-119 0.10638377680028555 2110 120-124 -3.170099947304294 2110 125-129 -0.008431671377891803 2110 130-134 0.15702983736318998 2110 135-139 0.17610076900808735 2110 140-144 0.13122959134069134 2110 145-149 -0.03480011902276914 2110 150-154 0.20987587125527085 2110 155-159 0.2864836495491616 2110 160-164 0.21538457491438123 2110 165-169 0.12064211042190465 2110 170-174 0.02730866202482929 2110 175-179 0.06765939474959737 2110 180-184 0.43897801927471747 2110 185-189 0.0035158650822992854 2110 190-194 -0.12336530145146085 2110 195-199 -0.13424132448393067 2110 200-204 -0.10269968008751462 2110 205-209 -0.24827337925476556 2110 210-214 -0.16086106295088243 2110 215-219 0.17651471052252177 2110 220-224 -0.047225176587371465 2110 225-229 0.1432984294113453 2110 230-234 0.10448337768661986 2110 235-239 0.10091812189551774 2110 240-244 -0.19112481928953073 2110 245-249 0.17968364691899907 2110 250-251 0.13768875135337666 2109 1 -0.023055558003704846 2109 2 -0.05154715526228415 2109 3 0.05351950540630668 2109 4 -0.18782506591010417 2109 5 -0.04789453604139737 2109 6 -0.12418777144497994 2109 7 -0.06180057004026196 2109 8 -0.08183979762317506 2109 9 -0.17971204223502468 2109 10-14 0.007532852109335408 2109 15-19 -0.10867405815653086 2109 20-24 -0.21569281152543596 2109 25-29 -0.3587647324264367 2109 30-34 -0.1737175686939949 2109 35-39 -0.5256786836494101 2109 40-44 -0.42029484902583647 2109 45-49 -0.38123581535946016 2109 50-54 -0.2131179176641922 2109 55-59 -0.23963380897638586 2109 60-64 -0.35145453073574373 2109 65-69 -0.2998417322601483 2109 70-74 -0.24466475852541691 2109 75-79 -0.2572777292567565 2109 80-84 -0.038001179978188304 2109 85-89 -0.1547744910805804 2109 90-94 0.035537742078794565 2109 95-99 -0.36193459915956083 2109 100-104 -0.3824045352844223 2109 105-109 -0.02842141577012569 2109 110-114 -0.07903998680800584 2109 115-119 -0.06619242634048561 2109 120-124 0.1601220362589757 2109 125-129 -0.1403637027395206 2109 130-134 -0.31423329336097794 2109 135-139 -0.0874154073065938 2109 140-144 0.017682878458181506 2109 145-149 -0.17577132015850339 2109 150-154 0.17415534653333253 2109 155-159 0.06099541016090626 2109 160-164 -0.045566299238089414 2109 165-169 -0.14133813414720464 2109 170-174 -0.16345880834893478 2109 175-179 -0.29039788032841685 2109 180-184 0.22393305561898913 2109 185-189 -0.2027498929390248 2109 190-194 -0.2986632922605317 2109 195-199 -0.261166165348083 2109 200-204 -0.190963728061039 2109 205-209 -0.25340467497318997 2109 210-214 -0.1449286951108455 2109 215-219 -0.19816793963672552 2109 220-224 -0.1276257702643413 2109 225-229 -0.04690555486173764 2109 230-234 -0.30564105018485677 2109 235-239 -0.010714581962002256 2109 240-244 -0.038684889903908726 2109 245-249 -0.006223669412186439 2109 250-251 -0.7726155541874569 2108 1 0.010674036707222001 2108 2 0.02998001459886268 2108 3 0.040525161313354374 2108 4 0.11683049113821653 2108 5 -0.11305422935734555 2108 6 -0.024126247815466684 2108 7 -0.15013281839737402 2108 8 -0.20597972092333094 2108 9 -0.05515177473697008 2108 10-14 -0.04017142853990663 2108 15-19 -0.1549391356261438 2108 20-24 -0.10112970137046773 2108 25-29 -0.13227532146760979 2108 30-34 -0.062326240931042776 2108 35-39 -0.10892799619348636 2108 40-44 -0.25528213202819217 2108 45-49 -0.17048030864866348 2108 50-54 0.030739723461039148 2108 55-59 0.01717297050090849 2108 60-64 0.07860694099941412 2108 65-69 0.14125420712264258 2108 70-74 0.13330955146717827 2108 75-79 0.042748390897102695 2108 80-84 0.2373791623284518 2108 85-89 -0.5087023452551591 2108 90-94 0.06732014562882682 2108 95-99 -0.015029203127866708 2108 100-104 0.2643574593727749 2108 105-109 0.1431796920011692 2108 110-114 0.1252920378268172 2108 115-119 0.17052916568577814 2108 120-124 0.5275230463535721 2108 125-129 0.009020974474772459 2108 130-134 0.010554502011544287 2108 135-139 0.09186041286911717 2108 140-144 0.15403538636773106 2108 145-149 0.26295812175241196 2108 150-154 0.10178752863865981 2108 155-159 0.05893255227010741 2108 160-164 0.02894660025467033 2108 165-169 0.06786065752179127 2108 170-174 0.03057495641861152 2108 175-179 0.22173046246027184 2108 180-184 0.1187945351959776 2108 185-189 -0.020976340257902848 2108 190-194 0.05663872418074334 2108 195-199 0.25390389490125287 2108 200-204 -0.6149227707372198 2108 205-209 -0.7810658025911827 2108 210-214 -1.661466673410935 2108 215-219 -1.2896715091027886 2108 220-224 -1.8529267430526737 2108 225-229 -0.5896210014443 2108 230-234 -0.3491456944404696 2108 235-239 -0.19251915592960955 2108 240-244 0.05007646702943447 2108 245-249 0.12196620392525048 2108 250-251 -0.10516634001199421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 6.0 18 32.0 19 193.0 20 568.0 21 2086.0 22 14215.0 23 25295.0 24 17926.0 25 10337.0 26 6384.0 27 4893.0 28 4945.0 29 5798.0 30 7054.0 31 9190.0 32 11626.0 33 14650.0 34 18786.0 35 26385.0 36 50021.0 37 83609.0 38 63415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.91094683571135 13.623994806777684 4.473060159241154 27.99199819826981 2 55.9076878237484 28.550799517772212 10.122544149013684 5.418968509465707 3 53.951750725328885 10.790509121259092 28.31577971198813 6.941960441423897 4 53.87199766834917 11.062623372150021 9.45934846256773 25.606030496933087 5 51.25683928831657 11.180265755203159 30.070082005219717 7.492812951260548 6 50.13658704608985 11.47702131605792 30.21501530145861 8.171376336393624 7 9.420929215849927 73.64996091835248 9.347005285958428 7.582104579839169 8 30.848535431819084 30.134467363512314 30.720294635878275 8.296702568790323 9 27.306280884437555 12.38768994343097 30.22905819853477 30.076970973596705 10-14 28.42753997588861 42.87794602758237 13.426758342938145 15.267755653590875 15-19 24.899964230356503 36.55371408131632 13.917941788217213 24.62837990010996 20-24 32.7753268947975 32.29823933865904 19.590477326020427 15.335956440523033 25-29 28.795728839606273 27.78273253580276 22.42783143224302 20.993707192347948 30-34 23.3831723699376 29.466608375395786 21.398725540850258 25.75149371381635 35-39 29.258614522475256 27.364254202933108 21.54095624180279 21.83617503278884 40-44 20.560603049693306 28.02305154803068 30.733860604374495 20.682484797901516 45-49 24.42626816634209 24.042234675357367 27.138190056039107 24.393307102261435 50-54 26.679914682776257 20.60490441556377 25.192374441927324 27.522806459732656 55-59 22.143211054144643 22.171031887974777 30.352794668998317 25.33296238888226 60-64 24.421021951962693 22.000344448418847 31.13368573056185 22.44494786905661 65-69 24.400196070638422 35.50023184028191 20.87760157916352 19.22197050991614 70-74 23.382377488971027 38.435992210166525 18.960348687784006 19.221281613078443 75-79 21.694792204867323 31.627094842547326 24.984698541393428 21.693414411191924 80-84 22.23123087317674 26.875243432296013 24.67358213107587 26.219943563451377 85-89 22.089159148417526 26.656068253778997 23.641296715816807 27.61347588198667 90-94 23.11429063497741 26.36047851834188 24.38170184014944 26.143529006531274 95-99 21.051097598134678 25.391571612151083 27.030616165229254 26.52671462448498 100-104 22.438323861001816 25.09041770994794 25.99732390074586 26.473934528304387 105-109 22.929189353894255 25.538783567160817 26.076917981532265 25.455109097412667 110-114 21.88148324788363 26.306267636421442 24.950624643959568 26.861624471735357 115-119 23.751255249526384 25.781964150868408 25.068426003206017 25.39835459639919 120-124 18.753520660281122 27.6563464621173 25.041612018600212 28.548520859001364 125-129 19.759310043321012 25.589602956957197 25.711643681358716 28.93944331836307 130-134 19.653760265893123 25.829458728168582 24.764794597350186 29.75198640858811 135-139 19.56221066286819 24.09129063437961 25.81666200335758 30.529836699394618 140-144 20.804737490560786 23.951035332458964 25.803054992514873 29.441172184465376 145-149 17.93469678454581 22.770278219084148 27.767167583253226 31.527857413116816 150-154 19.16473908032272 23.547606745889805 28.278685266881286 29.00896890690619 155-159 17.636669256213587 23.30941976838216 30.448885417318028 28.605025558086222 160-164 18.053134111327907 25.45045928246587 28.7204953276872 27.775911278519022 165-169 18.12111336327385 24.493939032629864 26.163348038631213 31.22159956546507 170-174 19.607498169123204 25.70646408142977 26.591961947438204 28.094075802008824 175-179 20.348380614644682 26.968894701532637 26.117311774736972 26.565412909085705 180-184 18.178026840480637 25.5811772187115 25.999231615065643 30.24156432574222 185-189 18.70604968326626 25.317290153963224 26.36573870447052 29.610921458299995 190-194 18.567697169107923 26.327757325763233 26.43755699964972 28.666988505479118 195-199 17.562100075513694 25.988686194242412 26.68325318283587 29.765960547408028 200-204 16.053733953340487 24.938595445332062 27.107348674535984 31.900321926791463 205-209 18.247889721277343 24.806194566929843 27.795904027030222 29.150011684762596 210-214 15.58109773061484 25.07250639216777 29.573493369367938 29.772902507849448 215-219 17.263763901150668 25.62490925104156 28.748687120907817 28.362639726899953 220-224 18.213155844775233 24.59749770808714 27.08447487374607 30.104871573391556 225-229 15.72120874898984 26.500536544652437 29.786627452538983 27.99162725381874 230-234 15.776964761318316 26.895394669207462 28.729292015045516 28.59834855442871 235-239 15.800016321564497 31.819048961513964 24.565756297845233 27.815178419076304 240-244 15.195376545911818 34.257268130449575 24.411920253259808 26.135435070378797 245-249 14.969728283189593 32.502046818488935 24.370255554230756 28.157969344090723 250-251 13.872262628671356 30.00225216273863 25.155465469046014 30.970019739544004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.3333333333333333 17 0.6666666666666666 18 1.0 19 2.333333333333333 20 2.6666666666666665 21 3.3333333333333335 22 4.333333333333333 23 9.666666666666666 24 18.000000000000004 25 28.999999999999964 26 44.33333333333337 27 84.33333333333321 28 250.00000000000273 29 450.9999999999918 30 750.3333333333487 31 1080.333333333367 32 1167.3333333333471 33 2073.666666666492 34 3223.000000000348 35 2448.999999999996 36 2963.333333333563 37 3427.0000000004407 38 3577.3333333338423 39 4065.3333333340643 40 5582.333333332726 41 5784.666666665876 42 7049.666666664725 43 7998.333333330529 44 8178.999999997031 45 8663.999999997877 46 9555.666666666166 47 8903.66666666498 48 9028.666666665207 49 9759.99999999987 50 8390.333333330713 51 7265.666666664529 52 6843.3333333315795 53 6904.666666664857 54 6320.666666665388 55 6047.6666666656365 56 6955.333333331478 57 5920.999999999085 58 4487.333333333722 59 4200.00000000065 60 9199.666666665518 61 6532.666666665195 62 1436.6666666666192 63 472.66666666665725 64 305.3333333333334 65 186.33333333333425 66 139.9999999999996 67 100.33333333333299 68 84.66666666666654 69 54.33333333333344 70 29.333333333333297 71 21.666666666666657 72 18.333333333333336 73 18.666666666666668 74 18.000000000000004 75 17.66666666666667 76 22.33333333333332 77 20.33333333333333 78 22.666666666666654 79 19.666666666666664 80 13.666666666666673 81 8.666666666666664 82 2.333333333333333 83 0.6666666666666666 84 0.3333333333333333 85 0.0 86 0.0 87 0.6666666666666666 88 0.6666666666666666 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 5.2992064438350364E-5 135-139 1.5897619331505108E-4 140-144 0.0 145-149 5.2992064438350364E-5 150-154 0.0 155-159 5.2992064438350364E-5 160-164 5.2992064438350364E-5 165-169 0.0 170-174 5.2992064438350364E-5 175-179 5.2992064438350364E-5 180-184 0.0 185-189 5.2992064438350364E-5 190-194 1.0598412887670073E-4 195-199 0.0 200-204 0.0 205-209 1.0598412887670073E-4 210-214 0.0 215-219 5.2992064438350364E-5 220-224 2.6496032219175175E-4 225-229 0.0 230-234 5.2992064438350364E-5 235-239 5.2992064438350364E-5 240-244 5.2992064438350364E-5 245-249 0.0 250-251 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 377415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.079505993229457 #Duplication Level Percentage of deduplicated Percentage of total 1 74.67738650380844 18.728719623809763 2 11.357755582579234 5.696937984058625 3 5.005185816760397 3.765827630660084 4 2.526972517839765 2.5350088962355413 5 1.3885653895094032 1.7412267004096038 6 1.0210607719823002 1.5364619850228955 7 0.694604548837038 1.219423726183806 8 0.5125902705616255 1.0284408610097118 9 0.4428867641906469 0.9996643131157196 >10 1.924775728226686 8.71387288717118 >50 0.15133678219511157 2.629706848401494 >100 0.2288288534381637 12.762183892249562 >500 0.03934167800983897 7.142423039774988 >1k 0.02658221487149641 11.500303763933752 >5k 0.0 0.0 >10k+ 0.0021265771897197128 19.99979784796335 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 64036 16.966999191871018 TruSeq Adapter, Index 2 (97% over 37bp) CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT 10963 2.904760012188175 No Hit GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT 4811 1.274724110064518 No Hit GGGGGGAGCAAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT 3492 0.9252414450935973 No Hit CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC 2583 0.6843925122212948 No Hit CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC 2503 0.6631956864459547 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG 2428 0.6433236622815733 No Hit CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG 2235 0.5921863200985652 No Hit CGGGTTATTAGTAGAAACAAGGGCTGGAGAATAGAACCGAGTCCAGAAAT 2118 0.5611859624021303 No Hit CGGGTTATTAGTAGAAACAAGGTTGGTTTGATTTAGTTAAAAGCGAAGTT 1584 0.4196971503517348 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 1487 0.39399599909913485 No Hit CGGGTTATTAGTAGAAACAAGGGAATAGAAACAGCTGTAAAAGGGCGCCT 1476 0.3910814355550256 No Hit GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA 1434 0.37995310202297206 No Hit CGGGTTATTAGTAGAAACAAGGTCAACATCACCCACAAAGAGTAAATTGT 1423 0.37703853847886276 No Hit CGGGTTATTAGTAGAAACAAGGTGTACTGCAGTAGAACTGAAAACGTATT 1395 0.36961964945749376 No Hit GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC 1392 0.3688247684909185 No Hit CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT 1386 0.367235006557768 No Hit CGGGTTATTAGTAGAAACAAGGAGGGAGGGGTGCCTGGGTGGCTCAGTCC 1250 0.3312004027396897 No Hit GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA 1247 0.3304055217731145 No Hit GGGGGGAGCGAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT 1219 0.32298663275174544 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 1171 0.31026853728654136 No Hit CGGGTTATTAGTAGAAACAAGGGTTAGAAGATTTCAGTTGGATTTCTGAA 1157 0.30655909277585686 No Hit CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC 1102 0.29198627505531044 No Hit GGGGGGAGCGAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC 1090 0.28880675118900945 No Hit GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 1051 0.2784732986235311 No Hit CGGGTTATTAGTAGAAACAAGGCTTCCAGGCTGCCAGGGTGGCTCAGTCG 1048 0.2776784176569559 No Hit CGGGTTATTAGTAGAAACAAGGCACACCATATTGACTTCATTTCATTTTT 1044 0.2766185763681889 No Hit GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC 969 0.2567465522038075 No Hit CGGGTTATTAGTAGAAACAAGGGCACCTAGGTGGCTCAGTCATTATGCAT 965 0.25568671091504047 No Hit CGGGTTATTAGTAGAAACAAGGGGTCAGCATGTAAGTGGCATGAAATATG 961 0.25462686962627346 No Hit GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA 920 0.24376349641641162 No Hit CGGGTTATTAGTAGAAACAAGGTAAGCTAATGAACGTAACTGTGATAAAG 919 0.2434985360942199 No Hit GGGGGGAGCAAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT 894 0.2368745280394261 No Hit CGGGTTATTAGTAGAAACAAGGGAACGCTATCCAGCAGTTAACAAAAAGA 851 0.2254812341851808 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT 841 0.22283163096326325 No Hit CGGGTTATTAGTAGAAACAAGGAGTACTTAGGGTGCCTATGTGGCTCAGT 824 0.2183273054860035 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 819 0.21700250387504472 No Hit CGGGTTATTAGTAGAAACAAGGACCTCATCTGAACCAGTTTCAAAAGAAA 800 0.21196825775340145 No Hit GGGGGGAGCAAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA 796 0.2109084164646344 No Hit CGGGTTATTAGTAGAAACAAGGAATGATCTAATATAAGATATGCTCTAGA 785 0.20799385292052516 No Hit CGGGTTATTAGTAGAAACAAGGGGACAGAGAGATTTAATATATAATCTAG 752 0.19925016228819734 No Hit CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC 736 0.19501079713312933 No Hit CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT 735 0.19474583681093757 No Hit CGGGTTATTAGTAGAAACAAGGACCCCTTTGAGCTCTGCTGCCAACAAGA 720 0.1907714319780613 No Hit CGGGTTATTAGTAGAAACAAGGGGCACCTCAGCGGCTCAGTGGGTTCAAG 712 0.18865174940052729 No Hit GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC 709 0.18785686843395202 No Hit CGGGTTATTAGTAGAAACAAGGGAGCATGTCACAAAGATCAGGACAGACT 683 0.18096790005696647 No Hit CGGGTTATTAGTAGAAACAAGGGAAAAGGATTTCCTCAGGGTCAGTTCCC 682 0.18070293973477472 No Hit GGGGGGAGCGAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC 667 0.17672853490189844 No Hit CGGGTTATTAGTAGAAACAAGGAAATTAAAAGGTGCTCTTCAGGAGACTT 657 0.17407893167998092 No Hit CGGGTTATTAGTAGAAACAAGGATTTGCTACTAGAATATCAAGAGACCCC 656 0.17381397135778917 No Hit CGGGTTATTAGTAGAAACAAGGTGGGACAGAGCAGCACCAGACTCATTTG 652 0.17275413006902215 No Hit CGGGTTATTAGTAGAAACAAGGTCGCAGGTGCCAATCAGAGCCTGCCCTG 644 0.17063444749148815 No Hit GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 641 0.1698395665249129 No Hit CGGGTTATTAGTAGAAACAAGGGATTAAAAATATTAAATGATAAGATAAA 632 0.16745492362518713 No Hit CGGGTTATTAGTAGAAACAAGGGGAAGAAGAGACTGTTCCGGGAATAGAA 627 0.1661301220142284 No Hit GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA 622 0.16480532040326962 No Hit GGGGGGAGCGAAAGCAGGAGGCAGGGAGAGGGAGAAGCAGGGTCTCTGCT 608 0.1610958758925851 No Hit CGGGTTATTAGTAGAAACAAGGGAAAATTCAAGAAGCTTTAGTAGCATCT 607 0.16083091557039333 No Hit GGGGGGAGCAAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC 576 0.15261714558244904 No Hit CGGGTTATTAGTAGAAACAAGGACAGTTGAGCTTAATGATCGTTCAACTT 575 0.1523521852602573 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCTGC 534 0.14148881205039546 No Hit CGGGTTATTAGTAGAAACAAGGGGTTAATCTGAGAAAGGAGAAAAAGGTC 508 0.1345998436734099 No Hit CGGGTTATTAGTAGAAACAAGGGCGCCTGGGTGGCTCAGTCTTAAGTGTC 505 0.13380496270683465 No Hit CGGGTTATTAGTAGAAACAAGGCTGGAGACTGTCGGGGACATGCATCCAG 497 0.13168528012930064 No Hit CGGGTTATTAGTAGAAACAAGGATAAAATTATTGTTAGAATGTAAAATTG 492 0.13036047851834187 No Hit GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC 489 0.1295655975517666 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA 485 0.12850575626299962 No Hit GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 484 0.12824079594080787 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGAGGGAGAGAGGCGGGCTTCCCGC 473 0.1253262323966986 No Hit CGGGTTATTAGTAGAAACAAGGGGAAAAGAATGTGCAGGGAGTATTAGAT 472 0.12506127207450685 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 463 0.12267662917478109 No Hit CGGGTTATTAGTAGAAACAAGGTGGAAAGCCGGGGCAACTCACTTCCAAG 462 0.12241166885258933 No Hit CGGGTTATTAGTAGAAACAAGGACATCAGGTGCCTCAGTCGGTTAGGCAT 448 0.11870222434190479 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGTGAGGGAGAAGCAGACTCCCTAC 446 0.1181723036975213 No Hit GGGGGGAGCAAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC 416 0.11022349403176873 No Hit GGGGGGAGCGAAAGCAGGGGGAATGGCAGACAGAGGGAGAAGCAGGCTCC 415 0.10995853370957698 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC 408 0.10810381145423473 No Hit GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA 405 0.10730893048765948 No Hit CGGGTTATTAGTAGAAACAAGGCGGTAGTGGATCAGAAAGGCCTTCAGAG 402 0.10651404952108422 No Hit GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 399 0.10571916855450895 No Hit GGGGGGAGCGAAAGCAGGCAGAATGTCAGACAGAGGCAGAGAGAGAAGCA 398 0.1054542082323172 No Hit GGGGGGAGCGAAAGCAGGAGAAGCAGCAGAGGGAGAGAGGGAAGCAGGCT 391 0.10359948597697495 No Hit GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG 378 0.10015500178848218 No Hit CGGGTTATTAGTAGAAACAAGGCAGCACAGTTGTGTGTTTTTCTCCAGAG 378 0.10015500178848218 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.948809665752554E-4 0.0 0.0 2 7.948809665752554E-4 0.0 0.0 3 7.948809665752554E-4 0.0 0.0 4 7.948809665752554E-4 0.0 0.0 5 7.948809665752554E-4 0.0 0.0 6 0.0013248016109587589 0.0 0.0 7 0.0015897619331505107 0.0 0.0 8 0.0021196825775340144 0.0 0.0 9 0.0021196825775340144 0.0 0.0 10-14 0.006571015990355445 0.0 0.0 15-19 0.010015500178848218 0.0 0.0 20-24 0.013248016109587587 0.0 0.0 25-29 0.01658651616920366 0.0 0.0 30-34 0.021991706741915397 0.0 0.0 35-39 0.02543619093040817 0.0 0.0 40-44 0.02824477034564074 0.0 0.0 45-49 0.03582263556032485 0.0 0.0 50-54 0.04901765960547408 0.0 0.0 55-59 0.07185723937840308 0.0 0.0 60-64 0.09474981121577045 0.0 0.0 65-69 0.1256441847833287 0.0 0.0 70-74 0.21281613078441505 0.0 0.0 75-79 0.3141899500549793 0.0 0.0 80-84 0.40470039611568165 0.0 0.0 85-89 0.5256282871639971 0.0 0.0 90-94 0.7275810447385505 0.0 0.0 95-99 1.113999178623001 0.0 0.0 100-104 1.5713736867904031 0.0 0.0 105-109 1.9566789873216486 0.0 0.0 110-114 2.415378297100009 0.0 0.0 115-119 3.0776731184505124 0.0 0.0 120-124 3.9378933004782533 0.0 0.0 125-129 4.671833392949405 0.0 0.0 130-134 5.541433170382736 0.0 0.0 135-139 6.478279877588331 0.0 0.0 140-144 7.666997867069407 0.0 0.0 145-149 8.8203701495701 0.0 0.0 150-154 10.123498006173575 0.0 0.0 155-159 12.731290489249234 0.0 0.0 160-164 14.574566458672814 0.0 0.0 165-169 17.445729502007076 0.0 1.0598412887670073E-4 170-174 24.853596173972946 0.0 2.649603221917518E-4 175-179 27.75427579719937 0.0 2.649603221917518E-4 180-184 29.61805969556059 0.0 2.649603221917518E-4 185-189 32.5901726216499 0.0 2.649603221917518E-4 190-194 35.06299431660109 0.0 2.649603221917518E-4 195-199 36.74475047361658 0.0 2.649603221917518E-4 200-204 38.65728707126108 0.0 2.649603221917518E-4 205-209 40.55980816872674 0.0 2.649603221917518E-4 210-214 42.45628817084641 0.0 4.239365155068029E-4 215-219 44.54173787475325 0.0 5.299206443835036E-4 220-224 46.33292264483394 0.0 5.299206443835036E-4 225-229 48.181815773087976 0.0 7.948809665752554E-4 230-234 49.480174343891996 0.0 7.948809665752554E-4 235-239 51.6687995972603 0.0 7.948809665752554E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 8070 0.0 242.72304 1 GTTATTA 8255 0.0 237.28346 4 TTATTAG 8310 0.0 235.5656 5 TATTAGT 8390 0.0 233.46544 6 TAGTAGA 8390 0.0 233.46544 9 GGTTATT 8450 0.0 231.95267 3 GGGGGGA 9215 0.0 231.70647 1 ATTAGTA 8460 0.0 231.6785 7 GGGTTAT 8655 0.0 226.31715 2 TTAGTAG 8735 0.0 224.24443 8 GGGAGCG 6355 0.0 208.76082 4 AGCGAAA 6340 0.0 208.48187 7 GCGAAAG 6350 0.0 207.57481 8 GGAGCGA 6460 0.0 205.36765 5 CGAAAGC 6460 0.0 203.47136 9 GGGGAGC 11440 0.0 186.21284 3 GGAGCAA 4360 0.0 184.87386 5 CAAAAGC 4390 0.0 181.65718 9 GCAAAAG 4400 0.0 181.52274 8 GGGGGAG 11765 0.0 181.2771 2 >>END_MODULE