FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated d3.3410000000852c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated d3.3410000000852c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377415
Sequences flagged as poor quality0
Sequence length251
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT6403616.966999191871018TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT109632.904760012188175No Hit
GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT48111.274724110064518No Hit
GGGGGGAGCAAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT34920.9252414450935973No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC25830.6843925122212948No Hit
CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC25030.6631956864459547No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG24280.6433236622815733No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG22350.5921863200985652No Hit
CGGGTTATTAGTAGAAACAAGGGCTGGAGAATAGAACCGAGTCCAGAAAT21180.5611859624021303No Hit
CGGGTTATTAGTAGAAACAAGGTTGGTTTGATTTAGTTAAAAGCGAAGTT15840.4196971503517348No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA14870.39399599909913485No Hit
CGGGTTATTAGTAGAAACAAGGGAATAGAAACAGCTGTAAAAGGGCGCCT14760.3910814355550256No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA14340.37995310202297206No Hit
CGGGTTATTAGTAGAAACAAGGTCAACATCACCCACAAAGAGTAAATTGT14230.37703853847886276No Hit
CGGGTTATTAGTAGAAACAAGGTGTACTGCAGTAGAACTGAAAACGTATT13950.36961964945749376No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC13920.3688247684909185No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT13860.367235006557768No Hit
CGGGTTATTAGTAGAAACAAGGAGGGAGGGGTGCCTGGGTGGCTCAGTCC12500.3312004027396897No Hit
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA12470.3304055217731145No Hit
GGGGGGAGCGAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT12190.32298663275174544No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11710.31026853728654136No Hit
CGGGTTATTAGTAGAAACAAGGGTTAGAAGATTTCAGTTGGATTTCTGAA11570.30655909277585686No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC11020.29198627505531044No Hit
GGGGGGAGCGAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC10900.28880675118900945No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA10510.2784732986235311No Hit
CGGGTTATTAGTAGAAACAAGGCTTCCAGGCTGCCAGGGTGGCTCAGTCG10480.2776784176569559No Hit
CGGGTTATTAGTAGAAACAAGGCACACCATATTGACTTCATTTCATTTTT10440.2766185763681889No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC9690.2567465522038075No Hit
CGGGTTATTAGTAGAAACAAGGGCACCTAGGTGGCTCAGTCATTATGCAT9650.25568671091504047No Hit
CGGGTTATTAGTAGAAACAAGGGGTCAGCATGTAAGTGGCATGAAATATG9610.25462686962627346No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA9200.24376349641641162No Hit
CGGGTTATTAGTAGAAACAAGGTAAGCTAATGAACGTAACTGTGATAAAG9190.2434985360942199No Hit
GGGGGGAGCAAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT8940.2368745280394261No Hit
CGGGTTATTAGTAGAAACAAGGGAACGCTATCCAGCAGTTAACAAAAAGA8510.2254812341851808No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT8410.22283163096326325No Hit
CGGGTTATTAGTAGAAACAAGGAGTACTTAGGGTGCCTATGTGGCTCAGT8240.2183273054860035No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG8190.21700250387504472No Hit
CGGGTTATTAGTAGAAACAAGGACCTCATCTGAACCAGTTTCAAAAGAAA8000.21196825775340145No Hit
GGGGGGAGCAAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA7960.2109084164646344No Hit
CGGGTTATTAGTAGAAACAAGGAATGATCTAATATAAGATATGCTCTAGA7850.20799385292052516No Hit
CGGGTTATTAGTAGAAACAAGGGGACAGAGAGATTTAATATATAATCTAG7520.19925016228819734No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC7360.19501079713312933No Hit
CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT7350.19474583681093757No Hit
CGGGTTATTAGTAGAAACAAGGACCCCTTTGAGCTCTGCTGCCAACAAGA7200.1907714319780613No Hit
CGGGTTATTAGTAGAAACAAGGGGCACCTCAGCGGCTCAGTGGGTTCAAG7120.18865174940052729No Hit
GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC7090.18785686843395202No Hit
CGGGTTATTAGTAGAAACAAGGGAGCATGTCACAAAGATCAGGACAGACT6830.18096790005696647No Hit
CGGGTTATTAGTAGAAACAAGGGAAAAGGATTTCCTCAGGGTCAGTTCCC6820.18070293973477472No Hit
GGGGGGAGCGAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC6670.17672853490189844No Hit
CGGGTTATTAGTAGAAACAAGGAAATTAAAAGGTGCTCTTCAGGAGACTT6570.17407893167998092No Hit
CGGGTTATTAGTAGAAACAAGGATTTGCTACTAGAATATCAAGAGACCCC6560.17381397135778917No Hit
CGGGTTATTAGTAGAAACAAGGTGGGACAGAGCAGCACCAGACTCATTTG6520.17275413006902215No Hit
CGGGTTATTAGTAGAAACAAGGTCGCAGGTGCCAATCAGAGCCTGCCCTG6440.17063444749148815No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG6410.1698395665249129No Hit
CGGGTTATTAGTAGAAACAAGGGATTAAAAATATTAAATGATAAGATAAA6320.16745492362518713No Hit
CGGGTTATTAGTAGAAACAAGGGGAAGAAGAGACTGTTCCGGGAATAGAA6270.1661301220142284No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA6220.16480532040326962No Hit
GGGGGGAGCGAAAGCAGGAGGCAGGGAGAGGGAGAAGCAGGGTCTCTGCT6080.1610958758925851No Hit
CGGGTTATTAGTAGAAACAAGGGAAAATTCAAGAAGCTTTAGTAGCATCT6070.16083091557039333No Hit
GGGGGGAGCAAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC5760.15261714558244904No Hit
CGGGTTATTAGTAGAAACAAGGACAGTTGAGCTTAATGATCGTTCAACTT5750.1523521852602573No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCTGC5340.14148881205039546No Hit
CGGGTTATTAGTAGAAACAAGGGGTTAATCTGAGAAAGGAGAAAAAGGTC5080.1345998436734099No Hit
CGGGTTATTAGTAGAAACAAGGGCGCCTGGGTGGCTCAGTCTTAAGTGTC5050.13380496270683465No Hit
CGGGTTATTAGTAGAAACAAGGCTGGAGACTGTCGGGGACATGCATCCAG4970.13168528012930064No Hit
CGGGTTATTAGTAGAAACAAGGATAAAATTATTGTTAGAATGTAAAATTG4920.13036047851834187No Hit
GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC4890.1295655975517666No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA4850.12850575626299962No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4840.12824079594080787No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGAGGGAGAGAGGCGGGCTTCCCGC4730.1253262323966986No Hit
CGGGTTATTAGTAGAAACAAGGGGAAAAGAATGTGCAGGGAGTATTAGAT4720.12506127207450685No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4630.12267662917478109No Hit
CGGGTTATTAGTAGAAACAAGGTGGAAAGCCGGGGCAACTCACTTCCAAG4620.12241166885258933No Hit
CGGGTTATTAGTAGAAACAAGGACATCAGGTGCCTCAGTCGGTTAGGCAT4480.11870222434190479No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGTGAGGGAGAAGCAGACTCCCTAC4460.1181723036975213No Hit
GGGGGGAGCAAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC4160.11022349403176873No Hit
GGGGGGAGCGAAAGCAGGGGGAATGGCAGACAGAGGGAGAAGCAGGCTCC4150.10995853370957698No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC4080.10810381145423473No Hit
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA4050.10730893048765948No Hit
CGGGTTATTAGTAGAAACAAGGCGGTAGTGGATCAGAAAGGCCTTCAGAG4020.10651404952108422No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG3990.10571916855450895No Hit
GGGGGGAGCGAAAGCAGGCAGAATGTCAGACAGAGGCAGAGAGAGAAGCA3980.1054542082323172No Hit
GGGGGGAGCGAAAGCAGGAGAAGCAGCAGAGGGAGAGAGGGAAGCAGGCT3910.10359948597697495No Hit
GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG3780.10015500178848218No Hit
CGGGTTATTAGTAGAAACAAGGCAGCACAGTTGTGTGTTTTTCTCCAGAG3780.10015500178848218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA80700.0242.723041
GTTATTA82550.0237.283464
TTATTAG83100.0235.56565
TATTAGT83900.0233.465446
TAGTAGA83900.0233.465449
GGTTATT84500.0231.952673
GGGGGGA92150.0231.706471
ATTAGTA84600.0231.67857
GGGTTAT86550.0226.317152
TTAGTAG87350.0224.244438
GGGAGCG63550.0208.760824
AGCGAAA63400.0208.481877
GCGAAAG63500.0207.574818
GGAGCGA64600.0205.367655
CGAAAGC64600.0203.471369
GGGGAGC114400.0186.212843
GGAGCAA43600.0184.873865
CAAAAGC43900.0181.657189
GCAAAAG44000.0181.522748
GGGGGAG117650.0181.27712
Produced by FastQC (version 0.11.2)