FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated c6.34100000008695.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated c6.34100000008695.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418759
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT8556320.4325160772664TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA76011.8151251674590874No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC73081.7451565220090792No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG65841.5722647155046219No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG64511.540504204088748No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC45141.077946981438011No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG39980.9547257491779281No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG37580.8974135481267268No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA28860.6891792176406955No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC22450.5361078806664454No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT22400.5349138764778787No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG21790.5203470253773651No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA20660.49336253071575775No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA20080.47951208212838414No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG16320.38972296714816873No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14250.3402911937415076No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC12130.28966541614627983No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA10510.250979680436719No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA10210.24381565530531882No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA10070.24047244357733205No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTCGTATCTCGTAT9510.22709959666538512TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGCATCTCGTAT8130.19414508106094436TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6280.14996692608397671No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA6050.1444745068165699No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA5700.13611647749660305No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGAATCTCGTAT5220.12465403728636279TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTTCGTATCTCGTAT4980.11892281718124267TruSeq Adapter, Index 21 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATATCGTAT4570.1091319828349958TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA34550.0244.290041
GGGGGGA32300.0244.240651
GGGTTAT34700.0243.234042
GGTTATT36200.0233.155293
TATTAGT36200.0232.47856
TTATTAG36300.0232.175525
ATTAGTA36700.0229.311227
GGGGGAG35000.0225.399252
GTTATTA38450.0219.51164
GGAGCGA23300.0219.237465
GCGAAAG23800.0214.631628
GAGCGAA23800.0214.631626
CGAAAGC23900.0213.733589
TAGTAGA39800.0211.758079
AGCGAAA24350.0209.783687
GGGGAGC38650.0204.113143
TTAGTAG42250.0199.188678
GGGAGCG26700.0190.860764
TCGGGAA150.0049658916163.332783
GAGCAAA18000.0155.84676
Produced by FastQC (version 0.11.2)