FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated c5.3410000000861e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated c5.3410000000861e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241833
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT10996645.47187521967639TruSeq Adapter, Index 13 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG131255.427299003858034No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT64672.6741594406057074No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA61012.5228153312409805No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT46761.9335657251078224No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA24671.0201254584775445No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT22000.9097186901704895No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA19540.8079956002696075No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA19480.8055145492964153No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG14970.6190222178114649No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11120.45982144703162925No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT10190.4213651569471495TruSeq Adapter, Index 13 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA9750.4031707831437397No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA8450.3494146787245744No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC7910.3270852199658442No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATTTCGTAT5530.2286701980292185TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG5400.223294587587302No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG4440.18359777201622607No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC3430.14183341396748997TruSeq Adapter, Index 13 (97% over 35bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACATTAATTG2860.11826342972216365No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA2720.11247431078471508No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAATCTCGTATG2480.10255010689194609TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA26800.0244.076141
GGGGGGA23350.0243.416461
GGGTTAT26950.0242.717642
GGTTATT27100.0241.37423
TTATTAG27200.0240.486795
TATTAGT27400.0238.731416
GTTATTA27700.0236.145874
ATTAGTA27950.0234.033667
GGGGGAG24400.0232.941592
TAGTAGA28200.0231.52459
TTAGTAG28600.0228.71478
GGGGAGC25500.0223.373473
GGAGCGA17500.0219.791295
GAGCGAA17850.0216.16796
GGGAGCG17900.0214.879764
GCGAAAG18150.0214.619588
CGAAAGC18400.0211.703579
AGCGAAA18900.0205.45487
GGGAGCA9400.0196.774114
GGAGCAA10450.0177.002555
Produced by FastQC (version 0.11.2)