FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated c4.34100000008596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated c4.34100000008596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16232
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1412887.03794972893051TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT2701.6633809758501725TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATTTCGTAT950.5852636766880237TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTTCCATCTCGTAT740.45588960078856583TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTTCCATCTCGTAT500.3080335140463282TruSeq Adapter, Index 10 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCACCTCGTAT430.2649088220798423TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATTCCATCTCGTAT380.23410547067520948TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG370.22794480039428291TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCTATCTCGTAT320.19714144898965008TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACTCCATCTCGTAT260.16017742730409068TruSeq Adapter, Index 7 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC250.1540167570231641TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGATTCCATCTCGTAT240.14785608674223755TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCAATCTCGTAT230.14169541646131098TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGCAT210.12937407589945785TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGTTCCATCTCGTAT190.11705273533760474TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACGAGATTCCATCTCGTATG170.1047313947757516Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC16400.0236.783546
CGGAAGA16550.0235.377644
GATCGGA16550.0235.377641
GGAAGAG16500.0235.348465
ATCGGAA16600.0234.668672
AAGAGCA16550.0234.637457
TCGGAAG16650.0233.963963
GAGCACA16550.0233.897289
AGAGCAC16550.0233.897288
CGGGTTA150.0049221204163.333331
GGAATCC150.0049221204163.33333245
TGACCAA150.0049221204163.33333245
TATTAGT150.0049221204163.333336
TTAGTAG150.0049221204163.333338
GGGTTAT150.0049221204163.333332
GGTTATT150.0049221204163.333333
TTATTAG150.0049221204163.333335
TAGTAGA150.0049221204163.333339
TTGGCAT150.00910127249.075-79
GCAAGCG150.00910127249.085-89
Produced by FastQC (version 0.11.2)