FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated c3.3410000000851f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated c3.3410000000851f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197993
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT9512648.0451329087392TruSeq Adapter, Index 2 (97% over 37bp)
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG22721.1475153161980474No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA21561.0889273863217386No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC18010.9096281181657938No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG12570.6348709297803458No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11660.5889097089291035No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG11570.5843640936800796No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT7790.3934482532210735No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG6140.3101119736556343No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC5870.29647512790856245No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA5850.2954649911865571No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4910.2479885652523069No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT4900.24748349689130422TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4760.240412539837267No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT4480.22627062572919246TruSeq Adapter, Index 11 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA4450.22475542064618448No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4190.21162364326011524No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT4190.21162364326011524TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATTTCGTAT3110.1570762602718278TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC2720.13737859419272397TruSeq Adapter, Index 2 (97% over 34bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2490.12576202188966276No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTCATTATCTCGTAT2440.12323668008464946TruSeq Adapter, Index 11 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG2290.11566065466960954TruSeq Adapter, Index 2 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG2200.11111503942058557No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC2150.10858969761557227No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA2110.10656942417156161No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA2010.101518740561535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA10350.0241.449281
GGGGGGA11800.0236.69491
GGGTTAT10700.0233.55142
GGTTATT10750.0232.465123
TTATTAG11400.0219.210535
TAGTAGA11550.0216.363639
ATTAGTA11650.0214.506447
GTTATTA11650.0214.506444
GGAGCGA9150.0211.530065
TATTAGT11850.0210.886066
GGGGGAG13250.0209.867942
GGGAGCG9500.0202.447364
CGAAAGC9550.0201.387449
GAGCGAA9650.0200.569956
GCGAAAG9600.0200.338558
GGGGAGC14400.0193.107623
TTAGTAG12950.0192.972988
AGCGAAA10050.0191.368167
GGATCGG150.0049638753163.333331
TCGGAAG166100.0155.097843
Produced by FastQC (version 0.11.2)