FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated c2.34100000008497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated c2.34100000008497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239242
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT28571.1941883114168916TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC14380.6010650303876409No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA13140.5492346661539362No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG12590.526245391695438No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG11000.4597854891699618No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA9430.3941615602611581No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG8310.3473470377274893No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG7950.3322995126273815No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA7920.3310455522023725No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG7810.32644769731067286No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT6800.28423102966870367No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT6330.26458564967689624No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG5730.2395064411767165No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA5330.2227869688432633No Hit
CGGGTTATTAGTAGAAACAAGGCCCGTGAGTCAGTAGTGAAACCAGAAGT4940.20648548331814648No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA4590.19185594502637496No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC4550.19018399779302964No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG4460.1864221165180027No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC4360.1822422484346394No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC4240.17722640673460346No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA4120.17221056503456753No Hit
CGGGTTATTAGTAGAAACAAGGTCAAAGAGCAGGTTCTGGGACAGTAGGA4070.17012063099288585No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA3610.15089323780941472No Hit
CGGGTTATTAGTAGAAACAAGGATGAAATACACTAGGCGAGCTTGCCAGA3150.1316658446259436No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT2890.12079818760919905No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT2530.10575066250909121No Hit
CGGGTTATTAGTAGAAACAAGGCAATAATAGGAGCGCCTGGGTGGCTCAG2410.10073482080905527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA18900.0239.81481
GGGTTAT19150.0236.684072
TATTAGT20200.0223.774776
ATTAGTA20350.0222.12537
TTATTAG20350.0222.12535
GTTATTA20550.0221.155724
TAGTAGA20350.0220.921379
GGTTATT21150.0214.30263
GGGGGGA43350.0206.003461
TTAGTAG22050.0204.444448
GGGAGCG27100.0197.084874
GGAGCGA27350.0194.835485
GGGGAGC45750.0192.519123
GAGCGAA27850.0191.777376
AGCGAAA28250.0188.628317
GCGAAAG28500.0186.973688
GGGGGAG48450.0183.054692
CGAAAGC29400.0181.250029
GGAGCAA20200.0172.227725
GAGCAAA20350.0169.152336
Produced by FastQC (version 0.11.2)