FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated c1.34100000008410.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated c1.34100000008410.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332420
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT6310018.9820107093436TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC75832.2811503519643823No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA53181.599783406533903No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC42501.2785030984898622No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG36881.1094398652307322No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT36781.1064316226460502TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG36541.0992118404428133No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG35001.05288490463871No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA29220.8790084832440889No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG25400.7640936165092354No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA22870.68798507911678No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG17600.5294506949040371No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT17060.5132061849467541No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG16430.49425425666325734No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC15900.47831057096444257No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA9270.27886408760002407No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT8550.25720474099031343No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC8160.24547259491005358No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA7990.2403585825160941No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATTTCGTAT7120.21418687202936046TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA6210.18681186450875398No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6190.18621021599181758No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4350.13085855243366826No Hit
GGGGGGAGCGAAAGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACAT4350.13085855243366826No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT4040.12153300042115397No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3920.11792310931953554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA31900.0244.615981
GGGTTAT32150.0242.713842
GGGGGGA22300.0242.253371
GGTTATT33500.0232.567173
TATTAGT34250.0227.474446
GGGGGAG23750.0227.463152
TTATTAG34400.0226.482565
ATTAGTA34700.0224.171487
GTTATTA35900.0217.019494
TAGTAGA35950.0216.036169
GGAGCGA16700.0215.658695
GCGAAAG17050.0211.950158
GAGCGAA17150.0209.999986
AGCGAAA17250.0208.782627
CGAAAGC17350.0208.285319
TTAGTAG37300.0208.217158
GGGGAGC26650.0203.63043
GGGAGCG19100.0188.560214
CGAGAGG7300.0132.5685245
GAGCAAA13800.0132.26456
Produced by FastQC (version 0.11.2)