##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE52Y l01n01 flu mrt-pcr updated b4.34100000008589.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71099 Sequences flagged as poor quality 0 Sequence length 251 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75051688490696 33.0 32.0 34.0 32.0 34.0 2 32.97572399049213 33.0 33.0 34.0 32.0 34.0 3 33.132603834090496 33.0 33.0 34.0 32.0 35.0 4 33.22157836256488 34.0 33.0 34.0 32.0 35.0 5 33.02902994416237 33.0 33.0 34.0 32.0 34.0 6 34.398247514029734 34.0 33.0 37.0 32.0 37.0 7 34.397516139467506 34.0 33.0 37.0 32.0 37.0 8 34.75466602905807 35.0 33.0 37.0 33.0 37.0 9 34.70105064768843 35.0 33.0 37.0 32.0 37.0 10-14 36.290355701205364 37.4 36.0 37.4 34.6 37.4 15-19 37.31680895652541 38.0 38.0 38.0 37.0 38.0 20-24 37.47460301832656 38.4 38.0 38.4 37.0 38.4 25-29 37.784964626787996 39.0 38.0 39.0 37.0 39.0 30-34 37.66478290833908 38.8 38.0 39.0 36.8 39.0 35-39 37.03343225643117 38.2 37.8 39.0 34.6 39.0 40-44 37.21364294856468 39.0 37.8 39.0 35.0 39.0 45-49 37.49048228526421 38.8 38.0 39.0 36.2 39.0 50-54 37.62458543720727 38.8 38.0 39.0 36.8 39.0 55-59 37.45323844217218 38.6 38.0 39.0 36.0 39.0 60-64 37.62319582553904 39.0 38.0 39.0 36.6 39.0 65-69 37.46319638813485 39.0 38.0 39.0 36.2 39.0 70-74 36.80512806087287 38.2 37.8 39.0 34.0 39.0 75-79 34.17620782289483 38.4 34.2 39.0 18.4 39.0 80-84 33.93639291691866 39.0 33.0 39.0 17.4 39.0 85-89 33.98497587870434 38.2 33.0 39.0 17.6 39.0 90-94 33.87080830954022 38.0 33.0 39.0 17.0 39.0 95-99 33.92639840222788 38.2 33.0 39.0 17.0 39.0 100-104 33.741478783105244 38.0 33.0 39.0 17.0 39.0 105-109 33.66482791600445 38.0 33.0 39.0 17.0 39.0 110-114 33.65834399921236 38.0 33.0 39.0 16.6 39.0 115-119 33.484620036850025 38.0 33.0 39.0 16.0 39.0 120-124 33.046627941321255 38.0 30.4 39.0 16.0 39.0 125-129 33.34817367332874 38.0 32.0 39.0 16.0 39.0 130-134 33.130983558137245 38.0 30.8 39.0 16.0 39.0 135-139 33.00130803527476 38.0 30.4 39.0 15.4 39.0 140-144 32.77028087596169 38.0 29.4 39.0 15.0 39.0 145-149 32.72452777113602 38.0 29.4 39.0 15.0 39.0 150-154 32.46918240762879 37.2 27.8 39.0 15.0 39.0 155-159 32.29688462566281 37.0 27.6 39.0 15.0 39.0 160-164 32.29015035373212 37.0 27.0 39.0 15.0 39.0 165-169 32.14123967988298 37.0 27.0 39.0 15.0 39.0 170-174 31.971780193814254 37.0 26.4 39.0 15.0 39.0 175-179 31.692828309821515 37.0 25.6 39.0 14.8 39.0 180-184 31.31988354266586 37.0 24.8 39.0 14.2 39.0 185-189 31.3892319160607 37.0 25.0 39.0 14.0 39.0 190-194 31.131090451342494 37.0 24.0 38.2 14.0 39.0 195-199 30.78380567940477 37.0 24.0 38.0 14.0 39.0 200-204 30.544024529177626 37.0 22.4 38.0 14.0 39.0 205-209 30.235398528811942 37.0 15.6 38.0 14.0 39.0 210-214 29.894642681331664 37.0 15.0 38.0 14.0 39.0 215-219 29.494282620008722 37.0 15.0 38.0 14.0 39.0 220-224 29.182193842388777 37.0 15.0 37.6 14.0 38.0 225-229 28.69539655972658 36.0 15.0 37.0 13.8 38.0 230-234 28.434450554860128 35.4 15.0 37.0 13.2 38.0 235-239 28.032892164446757 33.6 15.0 37.0 13.0 38.0 240-244 27.281193828323886 32.6 14.6 37.0 13.0 38.0 245-249 27.036838774103717 32.4 14.0 37.0 13.0 37.6 250-251 25.408950899450062 28.0 14.0 36.5 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3798697758765144 1101 2 -0.1425007911854621 1101 3 -0.11998531927895328 1101 4 -0.061547055691399066 1101 5 0.054412360298144336 1101 6 0.20735806610709773 1101 7 -0.30684456619616896 1101 8 -0.07292009786450393 1101 9 0.015979810237041647 1101 10-14 0.1863465617795228 1101 15-19 -0.11210606887659935 1101 20-24 0.06831606237651755 1101 25-29 0.09755951776649141 1101 30-34 0.041851730457402425 1101 35-39 -0.07859190494093582 1101 40-44 -0.08711538803780883 1101 45-49 -0.04231913375198815 1101 50-54 -0.09108105395194599 1101 55-59 -0.2390143573008885 1101 60-64 -0.004314316772976667 1101 65-69 -0.12169857565984188 1101 70-74 -0.16565122354921158 1101 75-79 -0.5278365678365518 1101 80-84 -0.8361576246875657 1101 85-89 -0.6513623579987993 1101 90-94 -0.4221558937175445 1101 95-99 -0.6561946142987978 1101 100-104 -0.829151600793864 1101 105-109 -0.8508292658843217 1101 110-114 -1.0234954920762789 1101 115-119 -0.9536134154463198 1101 120-124 -0.45207508577268385 1101 125-129 -0.8516107774307144 1101 130-134 -0.7543300234374968 1101 135-139 -0.5671069394857682 1101 140-144 -0.6518544314714063 1101 145-149 -0.8113541815206347 1101 150-154 -0.8261121434774417 1101 155-159 -0.5755830506900068 1101 160-164 -0.5235059541926006 1101 165-169 -0.5780162950008929 1101 170-174 -0.514689640286015 1101 175-179 -0.5547406258525278 1101 180-184 -0.6079656229807036 1101 185-189 -1.165329123995864 1101 190-194 -0.9128098134205658 1101 195-199 -1.1175676687402287 1101 200-204 -1.0869814835927123 1101 205-209 -0.9731496539242599 1101 210-214 -0.9491261090431742 1101 215-219 -0.6929889709563426 1101 220-224 -0.9030396403399941 1101 225-229 -0.5139275271145713 1101 230-234 -0.7280883567381125 1101 235-239 -0.6908064183129987 1101 240-244 -1.052468459245251 1101 245-249 -0.5389093145762871 1101 250-251 -0.9149104110786688 1102 1 0.11720812143026649 1102 2 0.07579477606868323 1102 3 -0.061161789867185234 1102 4 -0.06023477980828318 1102 5 0.04730165609428383 1102 6 -0.015118473146735312 1102 7 -0.07069594332289597 1102 8 -0.012524889197379707 1102 9 -0.09605192852850308 1102 10-14 0.2353463328941956 1102 15-19 0.09424168423242918 1102 20-24 -0.043477635647434454 1102 25-29 0.13818818946862166 1102 30-34 0.04167167399901217 1102 35-39 -0.08296514061652971 1102 40-44 -0.06486468230804832 1102 45-49 0.06926093796541721 1102 50-54 0.004019274117027294 1102 55-59 0.11243753863258377 1102 60-64 0.0584979209625871 1102 65-69 9.198724976329231E-4 1102 70-74 -0.02221641772025862 1102 75-79 -0.477332257162864 1102 80-84 -0.3292834353231058 1102 85-89 -0.35086033617839973 1102 90-94 -0.40253584336277015 1102 95-99 -0.19921437473215775 1102 100-104 -0.19671397204586327 1102 105-109 -0.35403823818919733 1102 110-114 -0.3583852456430776 1102 115-119 -0.24821019579270143 1102 120-124 0.06005431073300116 1102 125-129 -0.4907532204001086 1102 130-134 -0.6585857646444779 1102 135-139 -0.652368250235746 1102 140-144 -0.17651758856304411 1102 145-149 -0.41174176067681145 1102 150-154 -0.42423528378414943 1102 155-159 -0.24584169111115983 1102 160-164 -0.2597446052950616 1102 165-169 -0.3545936963894576 1102 170-174 -0.2114226836979327 1102 175-179 -0.06319870168987052 1102 180-184 -0.06977992068422623 1102 185-189 -0.3370846744650784 1102 190-194 -0.0628487239264075 1102 195-199 -0.2629769920026028 1102 200-204 -0.09734235946057268 1102 205-209 -0.226977379567046 1102 210-214 -0.5032910590698805 1102 215-219 -0.3766175663633753 1102 220-224 -0.28735031402581157 1102 225-229 0.12441839491182805 1102 230-234 -0.07390967359170375 1102 235-239 -0.3557121938527814 1102 240-244 0.3077817887138501 1102 245-249 0.14089231813905911 1102 250-251 0.2925054735631285 1103 1 -0.2611184763844321 1103 2 -0.3644985931874203 1103 3 -0.1810540215690324 1103 4 0.07266448676857351 1103 5 0.04117981804608917 1103 6 -0.21314272101382414 1103 7 -0.11808405887445872 1103 8 -0.029034393762565003 1103 9 0.09391963261504088 1103 10-14 0.10147116450622917 1103 15-19 0.06786838682186414 1103 20-24 -0.10241492048503886 1103 25-29 -0.056641925783580405 1103 30-34 -0.09396969705321823 1103 35-39 -0.06069901279135337 1103 40-44 0.08126314286782588 1103 45-49 -0.08557217085063229 1103 50-54 0.03629887297947221 1103 55-59 -0.001039485764962933 1103 60-64 -0.09837081126313052 1103 65-69 -0.16242094474529267 1103 70-74 0.030380498908982645 1103 75-79 0.11759664569597561 1103 80-84 -0.15269310453430762 1103 85-89 -0.25315639964195213 1103 90-94 -0.15577338265330098 1103 95-99 -0.5043812659161091 1103 100-104 -0.25120880814824176 1103 105-109 -0.8947387321272302 1103 110-114 -0.41283817185173177 1103 115-119 -0.8734399518172538 1103 120-124 -0.47910749139241915 1103 125-129 -0.5494989171520999 1103 130-134 -0.7258451569510243 1103 135-139 -0.6054501242067118 1103 140-144 -0.42753090992211185 1103 145-149 -0.48712867880284705 1103 150-154 -0.5730453421586468 1103 155-159 -0.3656980000469474 1103 160-164 -0.8744519844299212 1103 165-169 -0.5185960912405285 1103 170-174 -0.6125243151902318 1103 175-179 -0.8782443535383955 1103 180-184 -0.7419098413547829 1103 185-189 -0.9168781185602768 1103 190-194 -0.7095662811783114 1103 195-199 -0.3885659756876798 1103 200-204 -0.8270385127310398 1103 205-209 -0.7009543291254943 1103 210-214 -0.5129423088827743 1103 215-219 -0.19599191152128626 1103 220-224 -0.43625317287927246 1103 225-229 -7.425091444588361E-4 1103 230-234 -1.0587712212642089 1103 235-239 -0.36417499882209725 1103 240-244 -0.20345904349689548 1103 245-249 -0.5174342796756406 1103 250-251 -0.41768761130441234 2112 1 -0.002437869261733283 2112 2 0.10652644616862972 2112 3 0.07067615932027138 2112 4 0.14856967582610991 2112 5 0.08943181555106605 2112 6 -0.0066497159940723805 2112 7 0.020003682831067238 2112 8 -0.3024920823002972 2112 9 -0.15911746602754562 2112 10-14 0.05716368240209846 2112 15-19 -0.03584147743691091 2112 20-24 0.0076156732714594 2112 25-29 0.07576963449799479 2112 30-34 -0.06009379483442956 2112 35-39 -0.2840393757580557 2112 40-44 -0.3330297849249675 2112 45-49 0.09075479620910443 2112 50-54 -0.006256151228214435 2112 55-59 0.1718805843333513 2112 60-64 0.1155300412036766 2112 65-69 0.3662391675308285 2112 70-74 0.06392076088659593 2112 75-79 -0.33561866900387116 2112 80-84 -0.25950392818951684 2112 85-89 -0.3322962101388782 2112 90-94 -0.499198695273968 2112 95-99 -0.24374325243109496 2112 100-104 -0.1879064646070887 2112 105-109 -0.7722300440944352 2112 110-114 -0.554234791687179 2112 115-119 -0.4462204193373722 2112 120-124 -0.020946681103417575 2112 125-129 -0.3270971587536664 2112 130-134 -0.24071134639468994 2112 135-139 -0.20056997450522118 2112 140-144 -0.265862213572575 2112 145-149 -0.29462266401090886 2112 150-154 -0.08058685164865409 2112 155-159 -0.1257776032191913 2112 160-164 -0.34996509816146926 2112 165-169 -0.28229645064291375 2112 170-174 -0.4275301072120925 2112 175-179 -0.10104412779805472 2112 180-184 -0.047654338934016494 2112 185-189 0.08214169312475406 2112 190-194 -0.28790708968515943 2112 195-199 0.03029225343876263 2112 200-204 0.004847310181602893 2112 205-209 0.3754884783714587 2112 210-214 0.19873381313581362 2112 215-219 0.2758826243743968 2112 220-224 0.22069576822031678 2112 225-229 0.03237597483094845 2112 230-234 0.012767674390289585 2112 235-239 0.21658657779595103 2112 240-244 0.22846539289482237 2112 245-249 -0.040854839868117665 2112 250-251 -0.2495407612732201 1113 1 0.08378358882652748 1113 2 -0.060456933917642175 1113 3 0.1358286342094175 1113 4 0.07346335060667286 1113 5 0.07343024706555923 1113 6 -0.15168938057947656 1113 7 0.10358586242266199 1113 8 0.11041172730968896 1113 9 -0.4131517917295966 1113 10-14 -0.07585293751164102 1113 15-19 -0.03307249725411765 1113 20-24 -0.019072710886838706 1113 25-29 0.13368294551057858 1113 30-34 -0.2212375827269284 1113 35-39 0.1410111475731597 1113 40-44 0.15188862366550637 1113 45-49 0.2157178463101559 1113 50-54 0.18750308624652234 1113 55-59 0.20745805846842558 1113 60-64 0.3623191703925883 1113 65-69 0.051426028448389616 1113 70-74 0.5278063820973387 1113 75-79 0.20185606933051048 1113 80-84 -0.019205915943260266 1113 85-89 0.29199420098530027 1113 90-94 0.44062877132031275 1113 95-99 -0.021175159045874636 1113 100-104 0.3350507338364892 1113 105-109 -0.23509614972589787 1113 110-114 -0.027377493705088796 1113 115-119 -0.14213632438367085 1113 120-124 0.5258454644037798 1113 125-129 -0.14544542286249396 1113 130-134 0.0739358951822382 1113 135-139 0.14944661524533132 1113 140-144 0.04269809771147237 1113 145-149 -0.34798435073609824 1113 150-154 0.47954948593769586 1113 155-159 0.06223856424679752 1113 160-164 0.04227089858090238 1113 165-169 0.11544734328222006 1113 170-174 0.44823075907451937 1113 175-179 -0.030211925584076482 1113 180-184 0.14157438848130255 1113 185-189 -0.09285302831824893 1113 190-194 0.13265515204258804 1113 195-199 0.16398151232935732 1113 200-204 -0.31170624454949447 1113 205-209 0.011590791887584828 1113 210-214 -0.40472288761524666 1113 215-219 0.22237012173511772 1113 220-224 -0.19025045082471692 1113 225-229 0.032626329248348185 1113 230-234 0.13622256535039057 1113 235-239 0.18080399401771174 1113 240-244 0.04120632131758839 1113 245-249 0.002661583984931326 1113 250-251 0.6957658474801356 2113 1 -0.009210079437870888 2113 2 -0.11259019624085198 2113 3 -0.20395478492780938 2113 4 1.4540279910590925E-4 2113 5 -0.22217896057986763 2113 6 0.03113208814648516 2113 7 0.20252662814844058 2113 8 0.1923396520389673 2113 9 0.13972115933259488 2113 10-14 -0.13593341564643424 2113 15-19 0.09687602040964549 2113 20-24 0.26857744592717125 2113 25-29 0.30824357039962536 2113 30-34 0.11519060829029826 2113 35-39 0.1828124375903215 2113 40-44 0.2537822268372878 2113 45-49 0.07167973754631873 2113 50-54 0.02332177374282196 2113 55-59 0.32720478904419537 2113 60-64 0.17949178415671696 2113 65-69 0.12078516212493895 2113 70-74 0.2158766821150948 2113 75-79 0.732863821268495 2113 80-84 0.8824213992824923 2113 85-89 0.8117290965412636 2113 90-94 0.9159823425375393 2113 95-99 0.7864584287403744 2113 100-104 0.938867527729613 2113 105-109 0.8487727182544518 2113 110-114 0.9222763319650653 2113 115-119 0.7769417275720656 2113 120-124 1.242266554409099 2113 125-129 1.117676655366985 2113 130-134 1.1336968277817974 2113 135-139 0.9243208681597039 2113 140-144 1.4923164183221616 2113 145-149 1.1556194128002133 2113 150-154 1.4093974059329568 2113 155-159 1.5449890228538123 2113 160-164 1.340815191142596 2113 165-169 1.3210985652480147 2113 170-174 1.3126665245044293 2113 175-179 1.3935113716524477 2113 180-184 1.7191588609352983 2113 185-189 1.4281600493786577 2113 190-194 1.7202047111880958 2113 195-199 1.5686859327092684 2113 200-204 1.3844118689483444 2113 205-209 0.9471372739279431 2113 210-214 0.8954546376821035 2113 215-219 0.7536264090893958 2113 220-224 0.7942811782657628 2113 225-229 0.10307428474867564 2113 230-234 0.8801600764457191 2113 235-239 1.0419318714069092 2113 240-244 1.2774569870374535 2113 245-249 0.8672985447518364 2113 250-251 -0.4653975349685311 1112 1 0.008981842412914887 1112 2 0.17423967126813977 1112 3 0.20185376118081422 1112 4 0.09680826729561431 1112 5 0.03382408115626134 1112 6 -0.12036976906740193 1112 7 0.028650461905861846 1112 8 0.1960394560809675 1112 9 0.13910696261416433 1112 10-14 0.13810946113400746 1112 15-19 0.05633318750040672 1112 20-24 0.14959876732755362 1112 25-29 0.20284448876996208 1112 30-34 0.24220356491630213 1112 35-39 0.5406814674519822 1112 40-44 0.3437357233714664 1112 45-49 0.32126003645539214 1112 50-54 0.27711370729258533 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210-214 -0.21341370573810536 2111 215-219 -0.16540281751563057 2111 220-224 0.06111308727096443 2111 225-229 -0.1747457328906954 2111 230-234 -0.2490178682639943 2111 235-239 -0.008945534998009208 2111 240-244 0.05666078254245477 2111 245-249 0.1497291277633046 2111 250-251 0.21725354012042786 2110 1 -0.08107217429458302 2110 2 0.053502908501982915 2110 3 0.1914900905571102 2110 4 -0.17968190291982467 2110 5 0.09627304792970648 2110 6 -0.18098153968482933 2110 7 -0.15759726122710305 2110 8 0.07914981347057193 2110 9 -0.09751448076251279 2110 10-14 -0.07751769796404773 2110 15-19 -0.03046098371999051 2110 20-24 0.08922567518509084 2110 25-29 -0.08013710786342187 2110 30-34 -0.152404185853122 2110 35-39 -0.2505125530241159 2110 40-44 -0.45932627022190076 2110 45-49 -0.15484385149134994 2110 50-54 -0.052438456516213705 2110 55-59 -0.10486879373642921 2110 60-64 -0.08008774281112352 2110 65-69 -0.3588294042622451 2110 70-74 -0.29047421264150586 2110 75-79 -0.43025093282985694 2110 80-84 -0.5658954571810284 2110 85-89 -0.43094391059176473 2110 90-94 -0.5269910024755973 2110 95-99 -0.6744538475704687 2110 100-104 -1.9083918891143306 2110 105-109 -0.6187344773405954 2110 110-114 -0.823412568047452 2110 115-119 -0.2744911343976497 2110 120-124 -3.2092288779523024 2110 125-129 -0.7093099545691928 2110 130-134 -0.16828039659561966 2110 135-139 -0.24383080246975908 2110 140-144 -0.40894124655081043 2110 145-149 -0.3865280030426135 2110 150-154 -0.677706836339599 2110 155-159 -0.48154082322302116 2110 160-164 -0.6858648238742973 2110 165-169 -0.6668566343515359 2110 170-174 -0.21565283477476527 2110 175-179 -0.6320736615098568 2110 180-184 -0.27582299365111496 2110 185-189 -0.801742340810307 2110 190-194 -0.7328487239264057 2110 195-199 -0.43197731344265833 2110 200-204 -0.6012157120803074 2110 205-209 -0.5084752902067073 2110 210-214 -0.4570294068680134 2110 215-219 -0.6746053516414214 2110 220-224 -0.9419533355622924 2110 225-229 -0.7645079953164 2110 230-234 -0.9350507857742798 2110 235-239 -0.6997462664982379 2110 240-244 -0.7851947461624 2110 245-249 -0.3136174857825118 2110 250-251 -0.036665211104182305 2109 1 0.4198636718394084 2109 2 0.20591674611654298 2109 3 0.033557987807661505 2109 4 -0.0674449079540338 2109 5 0.12244446912500706 2109 6 -0.41900916328106064 2109 7 0.013339875729137418 2109 8 -0.06464778560003026 2109 9 8.139044188482103E-4 2109 10-14 0.14517495725733198 2109 15-19 -0.10884616508052147 2109 20-24 -0.1472787781516871 2109 25-29 -0.23928903764268483 2109 30-34 -0.1690493388155616 2109 35-39 -0.755650025594143 2109 40-44 -0.23195438433058513 2109 45-49 -0.299486939138184 2109 50-54 0.030871211742464766 2109 55-59 -0.2932255770073766 2109 60-64 -0.11602423435623166 2109 65-69 -0.1279916606195428 2109 70-74 -0.1173684942989297 2109 75-79 -0.5868212181015764 2109 80-84 -0.6208844851918727 2109 85-89 -0.5771811637220097 2109 90-94 -0.5465469596202936 2109 95-99 -0.5329861826679263 2109 100-104 -1.1429020220690305 2109 105-109 -0.8806616749654808 2109 110-114 -0.7974928571916351 2109 115-119 -0.807484551204162 2109 120-124 -0.325738494578097 2109 125-129 -0.7955949681228205 2109 130-134 -0.5089671140346326 2109 135-139 -0.6797543857923358 2109 140-144 -0.7935223747294771 2109 145-149 -0.9705992564310186 2109 150-154 -0.7410023055534865 2109 155-159 -0.7309922237651634 2109 160-164 -0.6735503795732782 2109 165-169 -0.9297519486667518 2109 170-174 -0.5835888099909781 2109 175-179 -0.46491863375718623 2109 180-184 -0.6699497523964375 2109 185-189 -0.5357877777536437 2109 190-194 -0.9111951806193161 2109 195-199 -0.9637057244404588 2109 200-204 -0.6895918576850626 2109 205-209 -0.9179886502170369 2109 210-214 -0.5119926184337835 2109 215-219 -0.02010683608701669 2109 220-224 0.10916967979848735 2109 225-229 0.08594379247242401 2109 230-234 0.0397200932756725 2109 235-239 0.024050041750118822 2109 240-244 -0.07965066458243086 2109 245-249 -0.1797592180659784 2109 250-251 0.021454060872063252 2108 1 0.1596943649395257 2108 2 0.06434007561141897 2108 3 0.02625974000958564 2108 4 -0.2249096616187174 2108 5 0.11122477474886239 2108 6 0.10276108974561282 2108 7 -0.11539872562158848 2108 8 -0.03417876814025789 2108 9 -0.0043213232429053505 2108 10-14 0.02583226639803371 2108 15-19 -0.049507831384083545 2108 20-24 0.0034354853321829637 2108 25-29 -0.23002695429639175 2108 30-34 0.07943343482983067 2108 35-39 0.1481656041715027 2108 40-44 -0.11657048715962759 2108 45-49 -0.10751345558647074 2108 50-54 0.1907417417602062 2108 55-59 0.025967725410851017 2108 60-64 0.22269849447263113 2108 65-69 0.2733030392030571 2108 70-74 0.1598497684379936 2108 75-79 0.07041322838312425 2108 80-84 0.16109864636331395 2108 85-89 -0.47801836144383714 2108 90-94 0.30514144098970775 2108 95-99 0.33455606625056333 2108 100-104 0.46548551644517744 2108 105-109 0.17985620496356347 2108 110-114 -0.06062503483938997 2108 115-119 0.5307962368198744 2108 120-124 0.7051818004640751 2108 125-129 0.033912361247566025 2108 130-134 -0.1439364582816225 2108 135-139 0.24205010208542177 2108 140-144 -0.08022680347429656 2108 145-149 0.16493963124754885 2108 150-154 0.31917322586477326 2108 155-159 0.34840150814483195 2108 160-164 0.24455051986962673 2108 165-169 0.5656419077336139 2108 170-174 0.30770620105754887 2108 175-179 0.7689209604042127 2108 180-184 1.0381794686050831 2108 185-189 0.989630043042478 2108 190-194 0.7120129361526466 2108 195-199 0.7648087942486548 2108 200-204 0.2391685294799828 2108 205-209 0.0029288437918637555 2108 210-214 -0.9460594075159747 2108 215-219 -0.7398292225673622 2108 220-224 -1.2192390220781952 2108 225-229 -0.2682499767846771 2108 230-234 0.18428764486137084 2108 235-239 0.3609645479901964 2108 240-244 0.6381545702375249 2108 245-249 0.5832204588815202 2108 250-251 0.3352164710055803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 12.0 19 26.0 20 103.0 21 407.0 22 3170.0 23 5587.0 24 4217.0 25 2275.0 26 1353.0 27 945.0 28 904.0 29 1048.0 30 1308.0 31 1727.0 32 2095.0 33 2470.0 34 3077.0 35 4020.0 36 6370.0 37 15593.0 38 14391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.95859294786143 16.702063320159212 7.316558601386798 20.022785130592556 2 42.42253758843303 37.037089129242325 12.959394646900801 7.580978635423846 3 40.50408585212169 14.208357360863022 35.50120254855905 9.786354238456237 4 40.59972714102871 14.104277134699503 12.00157526828788 33.29442045598391 5 53.62944626506702 13.86939338106021 22.273168398992954 10.227991954879815 6 53.020436293056164 14.008635845792487 22.117048059747678 10.853879801403677 7 11.589473832262057 66.10922797788999 11.903121000295362 10.398177189552596 8 29.13683736761417 37.57999409274392 22.719025584044783 10.564142955597125 9 34.08768055809505 14.543101872037582 22.88499135008931 28.48422621977806 10-14 26.30782430132632 39.75161394675031 14.19288597589277 19.747675776030604 15-19 25.9283534226923 30.63249834737479 17.887171408880576 25.551976821052335 20-24 27.208258906595027 33.01255995161676 21.930266248470442 17.84891489331777 25-29 24.39865539599713 28.417277317543142 23.849843176415984 23.334224110043742 30-34 24.131703680783133 27.913472763330006 24.262225910350356 23.692597645536505 35-39 25.86674918071984 30.91717183082744 27.647927537658756 15.568151450793962 40-44 16.90234743104685 27.858338373254192 23.419176078425856 31.8201381172731 45-49 22.26247907846805 24.696831179060183 28.973965878563696 24.06672386390807 50-54 26.793907087300806 21.491441511132365 24.218062138708 27.496589262858826 55-59 18.665522721838563 20.11308175923712 34.63452369231635 26.58687182660797 60-64 23.44927495464071 20.180874555197683 33.70708448782683 22.662766002334774 65-69 22.309455829195908 38.70490442903557 20.594663778674803 18.390975963093716 70-74 19.727422326615002 41.400019690853604 21.007890406334827 17.86466757619657 75-79 18.5904161802557 35.67616984767718 25.24930027145248 20.484113700614635 80-84 20.51674425800644 29.692119439091968 24.50386081379485 25.287275489106737 85-89 20.135585591921124 26.93343084994163 25.394449992264306 27.53653356587294 90-94 22.026751431103108 28.09884808506449 25.80092547011913 24.07347501371327 95-99 21.35585591921124 25.823148004894588 28.705045077989844 24.11595099790433 100-104 20.517869449640642 26.647913472763328 25.82989915469979 27.00431792289624 105-109 22.97922614945358 27.28674102308049 25.679686071534057 24.054346755931867 110-114 21.9884949155403 26.513734370384956 27.143279089719968 24.354491624354775 115-119 21.23321003108342 27.521343478811232 26.454943107497996 24.79050338260735 120-124 19.113911588067342 27.43864189369752 25.347754539445 28.099691978790137 125-129 18.833738871151493 25.904443100465546 27.391102547152563 27.870715481230395 130-134 20.535872515787844 26.683357009240638 26.859168202084415 25.9216022728871 135-139 20.712585866428128 24.768913118083567 25.367516751337575 29.150984264150733 140-144 20.29316950497055 25.080029480103743 25.190579869139846 29.43622114578586 145-149 19.41557217722993 25.264913795770944 27.091110092181843 28.22840393481728 150-154 18.466082504676578 27.293492172885696 25.754511315208372 28.485914007229358 155-159 18.74467289088674 25.022996233399812 28.913930794699194 27.318400081014254 160-164 18.60762769554479 28.55097413739754 27.525077779090505 25.316320387967167 165-169 19.190987215010054 26.92049114614833 26.951152618180284 26.937369020661333 170-174 18.941529252251797 27.678385570501895 26.459799602806232 26.920285574440072 175-179 17.994627209946696 27.25101618869464 27.127526406841163 27.626830194517503 180-184 18.770833743561756 25.911340026385894 28.233467325657607 27.08435890439474 185-189 17.67737289518248 25.622654672090107 28.506810241523063 28.19316219120435 190-194 17.609066845198075 26.966775717102724 28.605626552421565 26.81853088527763 195-199 17.545957045809367 28.917706296853684 26.798970449654707 26.737366207682246 200-204 17.56654120744654 27.053902456862843 27.368394403281066 28.01116193240955 205-209 17.846996011184434 26.556566355550302 28.34534478781639 27.25109284544887 210-214 19.344575873078384 26.74130437840195 28.029648799561173 25.884470948958494 215-219 17.645304843401014 28.6679943965299 27.112131287729184 26.5745694723399 220-224 16.962311600195783 28.158281152424514 27.135479079815696 27.743928167564007 225-229 18.76257050028833 28.45553383310595 26.830475815412314 25.951419851193407 230-234 17.639960168104103 29.41259205499952 26.578507654137624 26.368940122758755 235-239 17.204180086921053 29.044009057792657 26.882234630585522 26.86957622470077 240-244 17.646661697070282 31.66964373619882 25.098524592469655 25.58516997426124 245-249 17.272816776607268 30.291002686395025 25.56266614157724 26.87351439542047 250-251 16.40670051618166 29.05174475027778 24.497531610852473 30.044023122688085 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.6666666666666666 18 1.0 19 0.3333333333333333 20 0.0 21 0.0 22 1.0 23 2.333333333333333 24 4.333333333333333 25 4.666666666666666 26 7.666666666666663 27 10.333333333333334 28 58.666666666666806 29 133.6666666666661 30 241.33333333333582 31 283.666666666668 32 253.33333333333616 33 298.3333333333338 34 359.33333333333036 35 575.9999999999956 36 717.6666666666783 37 631.3333333333352 38 723.0000000000123 39 839.3333333333588 40 1087.000000000032 41 1148.3333333333514 42 1455.3333333332816 43 1830.6666666665296 44 2103.6666666665055 45 2039.3333333331489 46 2221.3333333332257 47 2237.333333333233 48 2430.3333333333208 49 2871.3333333335213 50 2302.666666666596 51 1507.666666666603 52 1051.6666666667068 53 888.6666666666978 54 918.0000000000344 55 1014.3333333333787 56 873.6666666666961 57 627.0000000000014 58 503.9999999999888 59 426.33333333332655 60 372.66666666666293 61 243.00000000000253 62 100.66666666666632 63 61.66666666666683 64 53.66666666666677 65 37.66666666666666 66 20.999999999999993 67 14.666666666666675 68 10.666666666666668 69 7.33333333333333 70 4.333333333333333 71 3.3333333333333335 72 1.0 73 0.3333333333333333 74 0.3333333333333333 75 0.0 76 0.6666666666666666 77 0.6666666666666666 78 0.0 79 0.0 80 0.3333333333333333 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.3333333333333333 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 2.8129790855004993E-4 140-144 2.8129790855004993E-4 145-149 5.625958171000999E-4 150-154 0.0 155-159 5.625958171000999E-4 160-164 2.8129790855004993E-4 165-169 0.0 170-174 2.8129790855004993E-4 175-179 0.0 180-184 5.625958171000999E-4 185-189 2.8129790855004993E-4 190-194 5.625958171000999E-4 195-199 0.0 200-204 2.8129790855004993E-4 205-209 2.8129790855004993E-4 210-214 0.0 215-219 2.8129790855004993E-4 220-224 2.8129790855004993E-4 225-229 0.0 230-234 2.8129790855004993E-4 235-239 0.0 240-244 0.0 245-249 0.0 250-251 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 71099.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.63744305835176 #Duplication Level Percentage of deduplicated Percentage of total 1 76.06127945644752 27.10629515574529 2 10.873778666261444 7.7502733609602465 3 4.3615686643491856 4.663054647625063 4 2.5521420032971385 3.6380726127731826 5 1.5959737523239772 2.8438211860534865 6 0.9668888448544839 2.0674467691354304 7 0.6881616268989339 1.7167024555487427 8 0.5432039711609554 1.5486720473055278 9 0.33949686032750254 1.0888920025569515 >10 1.7800118791038229 10.747031083319046 >50 0.12270538503738006 3.1188694116372497 >100 0.09103946443331747 7.1433526642317915 >500 0.015832950336228446 3.850978035812084 >1k 0.003958237584057112 1.9508800818784293 >5k 0.0 0.0 >10k+ 0.003958237584057112 20.765658485417468 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 14721 20.704932558826425 TruSeq Adapter, Index 7 (97% over 38bp) GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 1383 1.9451750376235952 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 856 1.2039550485942137 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 695 0.9775102322114235 No Hit GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC 607 0.8537391524494016 No Hit GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC 572 0.8045120184531428 No Hit CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT 416 0.5850996497841038 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT 401 0.5640023066428501 No Hit CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC 370 0.5204011308175924 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC 325 0.45710910139383115 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT 323 0.4542961223083306 TruSeq Adapter, Index 7 (97% over 38bp) GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC 266 0.3741262183715664 No Hit GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA 264 0.3713132392860659 No Hit GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 262 0.3685002602005654 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 240 0.3375574902600599 No Hit GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC 214 0.3009887621485534 No Hit GGGGGGAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT 213 0.29958227260580317 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA 213 0.29958227260580317 No Hit CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC 212 0.2981757830630529 No Hit CGGGTTATTAGTAGAAACAAGGTAGGGGGGAAACAGGCTGATGACATATT 167 0.2348837536392917 No Hit CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT 155 0.21800587912628872 No Hit GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 148 0.20816045232703698 No Hit CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC 142 0.19972151507053545 No Hit GGGGGGAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT 140 0.19690853598503494 No Hit GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA 132 0.18565661964303296 No Hit GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC 128 0.18003066147203195 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATTTCGTAT 116 0.16315278695902896 TruSeq Adapter, Index 7 (97% over 38bp) CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC 113 0.15893331833077823 No Hit CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG 104 0.14627491244602595 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 98 0.13783597518952445 No Hit GGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCA 97 0.13642948564677423 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTTCCATCTCGTAT 93 0.1308035274757732 TruSeq Adapter, Index 7 (97% over 36bp) GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA 92 0.12939703793302298 No Hit GGGGGGAGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTT 88 0.12377107976202197 No Hit GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG 87 0.12236459021927172 No Hit GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT 86 0.12095810067652146 No Hit GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC 85 0.11955161113377122 No Hit GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT 85 0.11955161113377122 No Hit CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC 81 0.11392565296277023 No Hit GGGGGGAGCGAAAGCAGGGGTAGTGGCAGACAAAGGGAGCAGTAGGCTCC 80 0.11251916342001997 No Hit CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT 80 0.11251916342001997 No Hit GGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAA 78 0.10970618433451948 No Hit GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA 74 0.10408022616351849 No Hit GGGGGGAGCGAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG 73 0.10267373662076823 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG 72 0.10126724707801796 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014064895427502496 0.0 0.0 2 0.0014064895427502496 0.0 0.0 3 0.0014064895427502496 0.0 0.0 4 0.0014064895427502496 0.0 0.0 5 0.002812979085500499 0.0 0.0 6 0.002812979085500499 0.0 0.0 7 0.005625958171000998 0.0 0.0 8 0.007032447713751248 0.0 0.0 9 0.007032447713751248 0.0 0.0 10-14 0.010126724707801796 0.0 0.0 15-19 0.018003066147203196 0.0 0.0 20-24 0.029254982489205196 0.0 0.0 25-29 0.04557026118510808 0.0 0.0 30-34 0.06076034824681078 0.0 0.0 35-39 0.06779279596056204 0.0 0.0 40-44 0.07679432903416364 0.0 0.0 45-49 0.10576801361481877 0.0 0.0 50-54 0.14121155009212508 0.0 0.0 55-59 0.1825623426489824 0.0 0.0 60-64 0.23629024318204195 0.0 0.0 65-69 0.292549824892052 0.0 0.0 70-74 0.39691134896412045 0.0 0.0 75-79 0.5488122195811475 0.0 0.0 80-84 0.7358753287669306 0.0 0.0 85-89 0.9896060422790758 0.0 0.0 90-94 1.313098637111633 0.0 0.0 95-99 1.7086034965330037 0.0 0.0 100-104 2.2531962474859 0.0 0.0 105-109 2.8309821516477025 0.0 0.0 110-114 3.4408360173842105 0.0 0.0 115-119 4.0971040380314765 0.0 0.0 120-124 4.862796945104714 0.0 0.0 125-129 5.707534564480513 0.0 0.0 130-134 6.59502946595592 0.0 0.0 135-139 7.526125543256586 0.0 0.0 140-144 8.462566280819702 0.0 0.0 145-149 9.530091843767142 0.0 0.0 150-154 10.596210917171831 0.0 0.0 155-159 11.99594931011688 0.0 0.0 160-164 13.547307275770404 0.0 0.0 165-169 14.934387262830702 0.0 0.0 170-174 16.660431229693806 0.0 0.0 175-179 18.316150719419404 0.0 0.0 180-184 19.77046090662316 0.0 0.0 185-189 21.45402888929521 0.0 0.0 190-194 22.98091393690488 0.0 0.0 195-199 24.32692442931687 0.0 0.0 200-204 25.81470906763808 0.0 0.0 205-209 27.811642920434885 0.0 0.0 210-214 29.439795215122576 0.0 0.0 215-219 30.58749068200678 0.0 0.0 220-224 31.816481244461947 0.0 0.0 225-229 32.927607983234644 0.0 0.0 230-234 34.0513931278921 0.0 0.0 235-239 35.12820152182169 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 670 0.0 243.17165 1 TTATTAG 680 0.0 237.79411 5 GTTATTA 685 0.0 236.0584 4 GGGGGGA 1410 0.0 233.70567 1 GGTTATT 700 0.0 232.75002 3 ATTAGTA 700 0.0 231.00002 7 TATTAGT 700 0.0 231.00002 6 GGGTTAT 710 0.0 229.47182 2 TAGTAGA 725 0.0 223.03447 9 GGAGCGA 1000 0.0 203.34999 5 GCGAAAG 1020 0.0 198.16177 8 AGCGAAA 1020 0.0 198.16177 7 GAGCGAA 1035 0.0 195.28986 6 TTAGTAG 835 0.0 195.11975 8 GGGAGCG 1045 0.0 194.59329 4 GGGGGAG 1710 0.0 193.42105 2 CGAAAGC 1085 0.0 186.29031 9 GGGGAGC 1795 0.0 183.57938 3 AGCAAAA 775 0.0 162.80646 7 GCAAAAG 770 0.0 159.0909 8 >>END_MODULE