FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated b4.34100000008589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated b4.34100000008589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71099
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1472120.704932558826425TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG13831.9451750376235952No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA8561.2039550485942137No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG6950.9775102322114235No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC6070.8537391524494016No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC5720.8045120184531428No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT4160.5850996497841038No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT4010.5640023066428501No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC3700.5204011308175924No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC3250.45710910139383115No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT3230.4542961223083306TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC2660.3741262183715664No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA2640.3713132392860659No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG2620.3685002602005654No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2400.3375574902600599No Hit
GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC2140.3009887621485534No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT2130.29958227260580317No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA2130.29958227260580317No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC2120.2981757830630529No Hit
CGGGTTATTAGTAGAAACAAGGTAGGGGGGAAACAGGCTGATGACATATT1670.2348837536392917No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT1550.21800587912628872No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG1480.20816045232703698No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC1420.19972151507053545No Hit
GGGGGGAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT1400.19690853598503494No Hit
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA1320.18565661964303296No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC1280.18003066147203195No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATTTCGTAT1160.16315278695902896TruSeq Adapter, Index 7 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC1130.15893331833077823No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG1040.14627491244602595No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT980.13783597518952445No Hit
GGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCA970.13642948564677423No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTTCCATCTCGTAT930.1308035274757732TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA920.12939703793302298No Hit
GGGGGGAGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTT880.12377107976202197No Hit
GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG870.12236459021927172No Hit
GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT860.12095810067652146No Hit
GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC850.11955161113377122No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT850.11955161113377122No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC810.11392565296277023No Hit
GGGGGGAGCGAAAGCAGGGGTAGTGGCAGACAAAGGGAGCAGTAGGCTCC800.11251916342001997No Hit
CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT800.11251916342001997No Hit
GGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAA780.10970618433451948No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA740.10408022616351849No Hit
GGGGGGAGCGAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG730.10267373662076823No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG720.10126724707801796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA6700.0243.171651
TTATTAG6800.0237.794115
GTTATTA6850.0236.05844
GGGGGGA14100.0233.705671
GGTTATT7000.0232.750023
ATTAGTA7000.0231.000027
TATTAGT7000.0231.000026
GGGTTAT7100.0229.471822
TAGTAGA7250.0223.034479
GGAGCGA10000.0203.349995
GCGAAAG10200.0198.161778
AGCGAAA10200.0198.161777
GAGCGAA10350.0195.289866
TTAGTAG8350.0195.119758
GGGAGCG10450.0194.593294
GGGGGAG17100.0193.421052
CGAAAGC10850.0186.290319
GGGGAGC17950.0183.579383
AGCAAAA7750.0162.806467
GCAAAAG7700.0159.09098
Produced by FastQC (version 0.11.2)