FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated b3.34100000008502.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated b3.34100000008502.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388218
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT7176718.486262872921916TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG56731.4612923666599693No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC46531.1985533901055592No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA39811.0254547702579477No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG37110.9559062176406039No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG26820.6908489559989491No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG26780.6898186070712847No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA23700.6104817396411295No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG18910.4870974555533232No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG15140.3899870691209578No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA14260.36731939271234204No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC13630.35109139710162846No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA13210.34027273336115277No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC12760.3286813079249288No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT10630.27381522752680193No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT8040.20710013446053505No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT7260.18700833037108017No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA7140.1839172835880871No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC6550.16871963690503788No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT5820.14991576897516343No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGAAGTGGATGATGGC5710.14708230942408645No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5360.1380667563070234No Hit
GGGGGGAGCGAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG4950.12750567979846375No Hit
GGGGGGAGCGAAAGCAGGTAACCGGACTGCTGTAGAACCCAAGGCCCTCC4660.12003565007289718No Hit
CGGGTTATTAGTAGAAACAAGGCTCCTCAAGAAATCATCTCATACATTCT4530.11668701605798804No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA4360.11230803311541454No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA4290.11050492249200192No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC4190.10792905017284102No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT4180.10767146294092494No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA4080.10509559062176406No Hit
CGGGTTATTAGTAGAAACAAGGTCACTTGCAGAGCATGGTGAGACCTGCT3980.10251971830260316No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT3890.10020143321535838TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA33750.0241.724931
GGGTTAT34500.0236.470062
TTATTAG35200.0231.419545
ATTAGTA35600.0229.163397
TATTAGT35900.0227.248376
TAGTAGA36000.0226.617149
GGTTATT36500.0223.51283
GTTATTA36750.0221.99234
GGGGGGA61900.0220.848561
TTAGTAG38000.0215.012288
GGGGGAG69100.0197.127762
GGAGCGA43400.0195.598045
GCGAAAG43150.0195.311878
AGCGAAA43400.0194.75137
GGGGAGC70150.0193.478673
GAGCGAA43950.0193.15036
GGGAGCG44000.0192.93084
CGAAAGC44700.0187.991249
GAGCAAA27300.0182.173176
GGAGCAA30100.0168.482535
Produced by FastQC (version 0.11.2)