FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated b2.3410000000848a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated b2.3410000000848a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607802
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT161562.658102474160993TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC31830.5236902807164174No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC30460.5011500455740521No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT29850.491113882481466No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC28370.4667638474371588No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC27160.4468560485157995No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG23500.38663906996028313No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA22460.3695282345237429No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG21710.35718868973777645No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA16610.27327978519320434No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG16480.2711409307636368No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA16130.2653824765301858No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT14300.2352739872524276No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG14120.23231249650379565No Hit
GGGGGGAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG10980.1806509356665493No Hit
GGGGGGAGCGAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG10750.17686680859885293No Hit
GGGGGGAGCGAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCA10450.17193099068446632No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC10400.1711083543654019No Hit
GGGGGGAGCGAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG9810.16140124580044157No Hit
GGGGGGAGCAAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCA8910.14659379205728182No Hit
GGGGGGAGCAAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG7840.12898937482930295No Hit
GGGGGGAGCGAAAGCAGGCTGGAAACTTCAGAGCAGTTGCTCGGATTACC7460.12273733880441327No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG7400.12175017522153596No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG6870.113030230239453No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC6760.11122043033751122No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT6760.11122043033751122No Hit
GGGGGGAGCAAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA6730.11072684854607256No Hit
CGGGTTATTAGTAGAAACAAGGCTTGGGACACCTAGGTGGTTCAGTCAGT6250.10282953988305403No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC6240.10266501261924114No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT6160.10134879450873806No Hit
GGGGGGAGCGAAAGCAGGGTGAGTGAGGGAGATGCGTGGGTTGGGTAACA6080.10003257639823494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA39750.0237.29561
GGGTTAT40800.0231.789222
TATTAGT41600.0227.037266
ATTAGTA42000.0224.583337
TTATTAG42450.0222.491175
GTTATTA42850.0220.700124
TAGTAGA44650.0211.25429
TTAGTAG45150.0209.186058
GGGGGGA112450.0205.128941
GGTTATT47600.0198.676453
GGAGCGA72100.0189.611655
GAGCGAA73050.0187.145786
AGCGAAA72500.0187.044837
GGGAGCG73350.0186.380364
GGGGGAG124900.0183.406722
GGGGAGC125500.0181.846623
GCGAAAG75050.0180.526328
GGAGCAA51950.0176.145345
CGAAAGC77000.0175.636359
GAGCAAA52500.0172.900016
Produced by FastQC (version 0.11.2)