FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated b1.34100000008403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated b1.34100000008403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243415
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT5530522.720456832980712TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC49392.0290450465254812No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA36601.5036049545015715No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC32271.3257194503214675No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT31661.3006593677464413TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG29441.2094571000143788No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG28561.1733048497422098No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG23710.9740566522194606No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA18970.7793274859807325No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG17320.7115420167204157No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA15010.6166423597559723No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG14750.6059610130846497No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG13430.5517326376763962No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13090.5377647227985128No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT10700.43957849762750856No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATTTCGTAT6620.27196351909290717TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA6580.27032023498962676No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC6230.25594149908592323No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA6210.255119857034283No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT5580.22923813240761662No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA4990.20499969188423062No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4530.18610192469650597No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3510.14419818006285562No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3210.13187354928825257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT23300.0244.998552
CGGGTTA23350.0244.473941
GGGGGGA18350.0242.328281
GGTTATT24100.0236.865833
TTATTAG24800.0229.686145
TATTAGT24800.0229.192186
GGAGCGA13300.0224.735525
ATTAGTA25250.0224.622447
GAGCGAA13400.0223.05846
GCGAAAG13350.0222.976218
CGAAAGC13450.0221.31849
TAGTAGA26000.0218.142949
AGCGAAA13650.0218.075647
GTTATTA26250.0217.46544
TTAGTAG26400.0214.837758
GGGGGAG20850.0213.272132
GGGAGCG15050.0198.603494
GGGGAGC22550.0196.650733
CGAGAGG4600.0159.78166245
GAGCAAA10000.0144.549156
Produced by FastQC (version 0.11.2)