##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE52Y l01n01 flu mrt-pcr updated a8.3410000000877a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23
Sequences flagged as poor quality	0
Sequence length	251
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78260869565217	0.0	0.0	0.0	0.0	0.0
2	33.52173913043478	0.0	0.0	0.0	0.0	0.0
3	32.95652173913044	0.0	0.0	0.0	0.0	0.0
4	33.73913043478261	0.0	0.0	0.0	0.0	0.0
5	33.04347826086956	0.0	0.0	0.0	0.0	0.0
6	33.21739130434783	0.0	0.0	0.0	0.0	0.0
7	32.69565217391305	0.0	0.0	0.0	0.0	0.0
8	32.69565217391305	0.0	0.0	0.0	0.0	0.0
9	33.65217391304348	0.0	0.0	0.0	0.0	0.0
10-14	35.73913043478261	0.0	0.0	0.0	0.0	0.0
15-19	36.99130434782608	0.0	0.0	0.0	0.0	0.0
20-24	36.95652173913043	0.0	0.0	0.0	0.0	0.0
25-29	37.33913043478261	0.0	0.0	0.0	0.0	0.0
30-34	33.92173913043478	0.0	0.0	0.0	0.0	0.0
35-39	25.713043478260868	0.0	0.0	0.0	0.0	0.0
40-44	28.878260869565214	0.0	0.0	0.0	0.0	0.0
45-49	32.48695652173913	0.0	0.0	0.0	0.0	0.0
50-54	31.973913043478255	0.0	0.0	0.0	0.0	0.0
55-59	32.83478260869565	0.0	0.0	0.0	0.0	0.0
60-64	33.01739130434783	0.0	0.0	0.0	0.0	0.0
65-69	32.47826086956522	0.0	0.0	0.0	0.0	0.0
70-74	31.356521739130436	0.0	0.0	0.0	0.0	0.0
75-79	25.295652173913044	0.0	0.0	0.0	0.0	0.0
80-84	26.913043478260867	0.0	0.0	0.0	0.0	0.0
85-89	26.939130434782605	0.0	0.0	0.0	0.0	0.0
90-94	26.791304347826088	0.0	0.0	0.0	0.0	0.0
95-99	24.4	0.0	0.0	0.0	0.0	0.0
100-104	23.72173913043478	0.0	0.0	0.0	0.0	0.0
105-109	24.808695652173913	0.0	0.0	0.0	0.0	0.0
110-114	23.869565217391305	0.0	0.0	0.0	0.0	0.0
115-119	23.956521739130434	0.0	0.0	0.0	0.0	0.0
120-124	23.086956521739133	0.0	0.0	0.0	0.0	0.0
125-129	23.121739130434783	0.0	0.0	0.0	0.0	0.0
130-134	23.660869565217393	0.0	0.0	0.0	0.0	0.0
135-139	22.72173913043478	0.0	0.0	0.0	0.0	0.0
140-144	22.869565217391305	0.0	0.0	0.0	0.0	0.0
145-149	21.82608695652174	0.0	0.0	0.0	0.0	0.0
150-154	21.547826086956523	0.0	0.0	0.0	0.0	0.0
155-159	20.66086956521739	0.0	0.0	0.0	0.0	0.0
160-164	21.01739130434783	0.0	0.0	0.0	0.0	0.0
165-169	21.130434782608695	0.0	0.0	0.0	0.0	0.0
170-174	21.40869565217391	0.0	0.0	0.0	0.0	0.0
175-179	21.208695652173912	0.0	0.0	0.0	0.0	0.0
180-184	20.443478260869565	0.0	0.0	0.0	0.0	0.0
185-189	20.095652173913045	0.0	0.0	0.0	0.0	0.0
190-194	21.02608695652174	0.0	0.0	0.0	0.0	0.0
195-199	20.48695652173913	0.0	0.0	0.0	0.0	0.0
200-204	20.939130434782605	0.0	0.0	0.0	0.0	0.0
205-209	20.417391304347824	0.0	0.0	0.0	0.0	0.0
210-214	19.330434782608698	0.0	0.0	0.0	0.0	0.0
215-219	19.973913043478262	0.0	0.0	0.0	0.0	0.0
220-224	19.808695652173913	0.0	0.0	0.0	0.0	0.0
225-229	19.556521739130435	0.0	0.0	0.0	0.0	0.0
230-234	18.73913043478261	0.0	0.0	0.0	0.0	0.0
235-239	18.643478260869564	0.0	0.0	0.0	0.0	0.0
240-244	19.417391304347824	0.0	0.0	0.0	0.0	0.0
245-249	19.14782608695652	0.0	0.0	0.0	0.0	0.0
250-251	18.673913043478258	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1110	1	0.0
1110	2	-0.5
1110	3	0.0
1110	4	0.0
1110	5	0.0
1110	6	0.0
1110	7	0.0
1110	8	0.0
1110	9	0.0
1110	10-14	1.8999999999999986
1110	15-19	0.0
1110	20-24	0.0
1110	25-29	-0.10000000000000142
1110	30-34	-0.20000000000000284
1110	35-39	0.0
1110	40-44	-0.8000000000000007
1110	45-49	-0.10000000000000142
1110	50-54	0.0
1110	55-59	0.20000000000000284
1110	60-64	-0.19999999999999574
1110	65-69	-0.29999999999999716
1110	70-74	-2.299999999999997
1110	75-79	0.8999999999999986
1110	80-84	0.8999999999999986
1110	85-89	2.1000000000000014
1110	90-94	2.8999999999999986
1110	95-99	-2.8000000000000007
1110	100-104	1.6000000000000014
1110	105-109	1.8000000000000007
1110	110-114	-0.3999999999999986
1110	115-119	1.1999999999999993
1110	120-124	-2.0
1110	125-129	0.1999999999999993
1110	130-134	-0.5
1110	135-139	2.3000000000000007
1110	140-144	-1.3000000000000007
1110	145-149	0.6999999999999993
1110	150-154	-1.1999999999999993
1110	155-159	0.09999999999999787
1110	160-164	-0.3999999999999986
1110	165-169	-2.3999999999999986
1110	170-174	-0.29999999999999716
1110	175-179	5.5
1110	180-184	-0.40000000000000036
1110	185-189	-0.6999999999999993
1110	190-194	0.8999999999999986
1110	195-199	1.4000000000000021
1110	200-204	0.0
1110	205-209	3.599999999999998
1110	210-214	0.6000000000000014
1110	215-219	-1.2000000000000028
1110	220-224	0.3999999999999986
1110	225-229	-1.799999999999999
1110	230-234	2.099999999999998
1110	235-239	-0.9000000000000004
1110	240-244	1.4000000000000021
1110	245-249	1.1000000000000014
1110	250-251	-0.25
2108	1	0.0
2108	2	0.5
2108	3	0.0
2108	4	0.0
2108	5	0.0
2108	6	0.0
2108	7	0.0
2108	8	0.0
2108	9	0.0
2108	10-14	-1.8999999999999986
2108	15-19	0.0
2108	20-24	0.0
2108	25-29	0.10000000000000142
2108	30-34	0.19999999999999574
2108	35-39	0.0
2108	40-44	0.8000000000000007
2108	45-49	0.10000000000000142
2108	50-54	0.0
2108	55-59	-0.20000000000000284
2108	60-64	0.20000000000000284
2108	65-69	0.29999999999999716
2108	70-74	2.3000000000000043
2108	75-79	-0.8999999999999986
2108	80-84	-0.9000000000000021
2108	85-89	-2.099999999999998
2108	90-94	-2.900000000000002
2108	95-99	2.8000000000000007
2108	100-104	-1.5999999999999979
2108	105-109	-1.7999999999999972
2108	110-114	0.40000000000000213
2108	115-119	-1.1999999999999993
2108	120-124	2.0
2108	125-129	-0.1999999999999993
2108	130-134	0.5
2108	135-139	-2.3000000000000007
2108	140-144	1.3000000000000007
2108	145-149	-0.6999999999999993
2108	150-154	1.1999999999999993
2108	155-159	-0.10000000000000142
2108	160-164	0.3999999999999986
2108	165-169	2.3999999999999986
2108	170-174	0.3000000000000007
2108	175-179	-5.5
2108	180-184	0.40000000000000036
2108	185-189	0.7000000000000028
2108	190-194	-0.9000000000000021
2108	195-199	-1.3999999999999986
2108	200-204	0.0
2108	205-209	-3.6000000000000014
2108	210-214	-0.5999999999999979
2108	215-219	1.1999999999999993
2108	220-224	-0.40000000000000213
2108	225-229	1.8000000000000007
2108	230-234	-2.1000000000000014
2108	235-239	0.9000000000000004
2108	240-244	-1.3999999999999986
2108	245-249	-1.0999999999999979
2108	250-251	0.25
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
20	1.0
21	5.0
22	6.0
23	3.0
24	2.0
25	1.0
26	0.0
27	0.0
28	1.0
29	0.0
30	0.0
31	0.0
32	0.0
33	1.0
34	0.0
35	1.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	82.6086956521739	8.695652173913043	0.0	8.695652173913043
2	8.695652173913043	78.26086956521739	13.043478260869565	0.0
3	8.695652173913043	8.695652173913043	73.91304347826086	8.695652173913043
4	13.043478260869565	4.3478260869565215	0.0	82.6086956521739
5	82.6086956521739	8.695652173913043	8.695652173913043	0.0
6	78.26086956521739	4.3478260869565215	8.695652173913043	8.695652173913043
7	8.695652173913043	82.6086956521739	0.0	8.695652173913043
8	8.695652173913043	78.26086956521739	4.3478260869565215	8.695652173913043
9	78.26086956521739	8.695652173913043	4.3478260869565215	8.695652173913043
10-14	23.47826086956522	37.391304347826086	4.3478260869565215	34.78260869565217
15-19	18.26086956521739	24.347826086956523	24.347826086956523	33.04347826086956
20-24	20.869565217391305	39.130434782608695	20.869565217391305	19.130434782608695
25-29	18.26086956521739	21.73913043478261	27.82608695652174	32.17391304347826
30-34	16.52173913043478	24.347826086956523	23.47826086956522	35.65217391304348
35-39	14.782608695652174	22.608695652173914	30.434782608695656	32.17391304347826
40-44	20.0	27.82608695652174	28.695652173913043	23.47826086956522
45-49	18.26086956521739	31.30434782608696	29.565217391304348	20.869565217391305
50-54	33.04347826086956	7.82608695652174	23.47826086956522	35.65217391304348
55-59	6.956521739130435	10.434782608695652	46.08695652173913	36.52173913043478
60-64	15.65217391304348	6.956521739130435	54.78260869565217	22.608695652173914
65-69	22.608695652173914	63.47826086956522	8.695652173913043	5.217391304347826
70-74	6.956521739130435	73.91304347826086	7.82608695652174	11.304347826086957
75-79	13.043478260869565	49.56521739130435	15.65217391304348	21.73913043478261
80-84	19.130434782608695	33.91304347826087	22.608695652173914	24.347826086956523
85-89	16.52173913043478	29.565217391304348	31.30434782608696	22.608695652173914
90-94	15.65217391304348	31.30434782608696	33.91304347826087	19.130434782608695
95-99	16.52173913043478	22.608695652173914	33.04347826086956	27.82608695652174
100-104	14.782608695652174	25.217391304347824	28.695652173913043	31.30434782608696
105-109	13.91304347826087	28.695652173913043	20.869565217391305	36.52173913043478
110-114	20.869565217391305	26.956521739130434	26.08695652173913	26.08695652173913
115-119	13.91304347826087	26.956521739130434	30.434782608695656	28.695652173913043
120-124	17.391304347826086	25.217391304347824	29.565217391304348	27.82608695652174
125-129	17.391304347826086	21.73913043478261	34.78260869565217	26.08695652173913
130-134	15.65217391304348	38.26086956521739	23.47826086956522	22.608695652173914
135-139	17.391304347826086	29.565217391304348	29.565217391304348	23.47826086956522
140-144	25.217391304347824	26.08695652173913	26.956521739130434	21.73913043478261
145-149	17.391304347826086	26.956521739130434	27.82608695652174	27.82608695652174
150-154	18.26086956521739	27.82608695652174	26.08695652173913	27.82608695652174
155-159	25.217391304347824	31.30434782608696	23.47826086956522	20.0
160-164	22.608695652173914	27.82608695652174	24.347826086956523	25.217391304347824
165-169	25.217391304347824	25.217391304347824	28.695652173913043	20.869565217391305
170-174	15.65217391304348	30.434782608695656	30.434782608695656	23.47826086956522
175-179	19.130434782608695	31.30434782608696	24.347826086956523	25.217391304347824
180-184	13.043478260869565	31.30434782608696	25.217391304347824	30.434782608695656
185-189	12.173913043478262	31.30434782608696	32.17391304347826	24.347826086956523
190-194	16.52173913043478	32.17391304347826	23.47826086956522	27.82608695652174
195-199	9.565217391304348	30.434782608695656	29.565217391304348	30.434782608695656
200-204	12.173913043478262	30.434782608695656	26.08695652173913	31.30434782608696
205-209	14.782608695652174	33.91304347826087	21.73913043478261	29.565217391304348
210-214	16.52173913043478	26.08695652173913	28.695652173913043	28.695652173913043
215-219	7.82608695652174	29.565217391304348	26.956521739130434	35.65217391304348
220-224	13.91304347826087	25.217391304347824	28.695652173913043	32.17391304347826
225-229	15.65217391304348	33.04347826086956	29.565217391304348	21.73913043478261
230-234	11.304347826086957	33.04347826086956	24.347826086956523	31.30434782608696
235-239	16.52173913043478	30.434782608695656	20.869565217391305	32.17391304347826
240-244	7.82608695652174	39.130434782608695	23.47826086956522	29.565217391304348
245-249	10.434782608695652	27.82608695652174	33.91304347826087	27.82608695652174
250-251	6.521739130434782	30.434782608695656	26.08695652173913	36.95652173913043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.3333333333333333
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.6666666666666666
39	1.3333333333333333
40	0.3333333333333333
41	0.3333333333333333
42	0.6666666666666666
43	1.3333333333333333
44	0.6666666666666666
45	1.0
46	1.0
47	1.0
48	1.6666666666666665
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.3333333333333333
56	0.3333333333333333
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	23.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.65217391304348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.45454545454545	91.30434782608695
2	4.545454545454546	8.695652173913043
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTTCGCTACACGACT	2	8.695652173913043	TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTGCGTATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 21 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGGCGTATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTTCGTAACTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 21 (97% over 37bp)
ATCCAAGCCATGAGTTTCATGTCCTTCTTTGAAAATACAAACTTTCTCTC	1	4.3478260869565215	No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT	1	4.3478260869565215	No Hit
AAACACCAGCCTCCCATTTCAGAATATACATCCGATCACAATTGGAAAAT	1	4.3478260869565215	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTATTAGTAGAAACAAGGAGGTTTTTGAACAAATTACGTGTCAATG	1	4.3478260869565215	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTGCGAATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 11 (97% over 37bp)
CTCATCGGAGGACTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAA	1	4.3478260869565215	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTCGAATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCGTATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTTCGCAACACGAAT	1	4.3478260869565215	TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGGAGAATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGCAGAGCACACGTCTGAACTCCAGTCACAAACACTGATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1	4.3478260869565215	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGTCGAATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGGAGTATCTCGTAT	1	4.3478260869565215	TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTTCGCTACACGAAG	1	4.3478260869565215	TruSeq Adapter, Index 21 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0	0.0	0.0
15-19	0.0	0.0	0.0
20-24	0.0	0.0	0.0
25-29	0.0	0.0	0.0
30-34	0.0	0.0	0.0
35-39	0.0	0.0	0.0
40-44	0.0	0.0	0.0
45-49	0.0	0.0	0.0
50-54	0.0	0.0	0.0
55-59	0.0	0.0	0.0
60-64	0.0	0.0	0.0
65-69	0.0	0.0	0.0
70-74	0.0	0.0	0.0
75-79	0.0	0.0	0.0
80-84	0.0	0.0	0.0
85-89	0.0	0.0	0.0
90-94	0.8695652173913043	0.0	0.0
95-99	4.3478260869565215	0.0	0.0
100-104	7.826086956521739	0.0	0.0
105-109	8.695652173913043	0.0	0.0
110-114	8.695652173913043	0.0	0.0
115-119	8.695652173913043	0.0	0.0
120-124	8.695652173913043	0.0	0.0
125-129	8.695652173913043	0.0	0.0
130-134	8.695652173913043	0.0	0.0
135-139	8.695652173913043	0.0	0.0
140-144	8.695652173913043	0.0	0.0
145-149	8.695652173913043	0.0	0.0
150-154	8.695652173913043	0.0	0.0
155-159	8.695652173913043	0.0	0.0
160-164	8.695652173913043	0.0	0.0
165-169	9.565217391304348	0.0	0.0
170-174	13.043478260869566	0.0	0.0
175-179	13.043478260869566	0.0	0.0
180-184	13.043478260869566	0.0	0.0
185-189	13.043478260869566	0.0	0.0
190-194	13.043478260869566	0.0	0.0
195-199	13.043478260869566	0.0	0.0
200-204	13.043478260869566	0.0	0.0
205-209	13.043478260869566	0.0	0.0
210-214	13.043478260869566	0.0	0.0
215-219	13.043478260869566	0.0	0.0
220-224	13.043478260869566	0.0	0.0
225-229	13.043478260869566	0.0	0.0
230-234	13.043478260869566	0.0	0.0
235-239	13.043478260869566	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE