FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a8.3410000000877a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a8.3410000000877a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTTCGCTACACGACT28.695652173913043TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTGCGTATCTCGTAT14.3478260869565215TruSeq Adapter, Index 21 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGGCGTATCTCGTAT14.3478260869565215TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT14.3478260869565215TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTTCGTAACTCGTAT14.3478260869565215TruSeq Adapter, Index 21 (97% over 37bp)
ATCCAAGCCATGAGTTTCATGTCCTTCTTTGAAAATACAAACTTTCTCTC14.3478260869565215No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT14.3478260869565215No Hit
AAACACCAGCCTCCCATTTCAGAATATACATCCGATCACAATTGGAAAAT14.3478260869565215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT14.3478260869565215TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTATTAGTAGAAACAAGGAGGTTTTTGAACAAATTACGTGTCAATG14.3478260869565215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTGCGAATCTCGTAT14.3478260869565215TruSeq Adapter, Index 11 (97% over 37bp)
CTCATCGGAGGACTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAA14.3478260869565215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT14.3478260869565215TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTCGAATCTCGTAT14.3478260869565215TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCGTATCTCGTAT14.3478260869565215TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTTCGCAACACGAAT14.3478260869565215TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGGAGAATCTCGTAT14.3478260869565215TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGCAGAGCACACGTCTGAACTCCAGTCACAAACACTGATCTCGTAT14.3478260869565215TruSeq Adapter, Index 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA14.3478260869565215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGTCGAATCTCGTAT14.3478260869565215TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCGGAGTATCTCGTAT14.3478260869565215TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTTCGCTACACGAAG14.3478260869565215TruSeq Adapter, Index 21 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)