FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a7.341000000086f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a7.341000000086f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574523
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT6036210.5064549199945TruSeq Adapter, Index 19 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC106971.8618923872499447No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA106091.8465753329283598No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG96451.6787839651328147No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG81841.4244860519074083No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG65961.1480828443769875No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC56910.9905608652743233No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA45810.7973571118997846No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT45480.7916132165291903No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG44890.7813438278363094No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG33510.5832664662685393No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA33500.5830924088330668No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC32130.5592465401733264No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT29380.5113807454183731No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA29120.5068552520960867No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG26820.4668220419373985No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC17940.3122590392377677No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA17600.3063410864317007No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA16960.29520141056145704No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA16920.29450518081956684No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15130.2633488998699791No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA10780.1876339154394167No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA9780.17022817189216097No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA5910.10286794436428133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA48600.0243.487661
CGGGTTA52400.0243.129791
GGGTTAT52750.0241.981052
GGTTATT54050.0236.38763
TATTAGT54750.0234.036516
TTATTAG54800.0233.152375
ATTAGTA55300.0231.708857
GTTATTA56950.0224.350314
GGGGGAG53700.0220.135032
TAGTAGA59650.0214.81149
GGAGCGA36450.0214.080925
GCGAAAG37150.0211.366078
AGCGAAA37400.0210.280737
GAGCGAA37250.0210.140956
CGAAAGC37450.0209.345819
TTAGTAG65200.0196.713968
GGGGAGC61400.0191.929963
GGGAGCG42950.0181.682194
GAGCAAA26750.0150.205616
CGAGAGG8750.0126.0245
Produced by FastQC (version 0.11.2)