FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a6.3410000000867b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a6.3410000000867b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517272
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT13360025.827804327317157TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG85761.6579285172984424No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA82101.5871727060424687No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC77231.4930249462565148No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG75801.4653799161756291No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA50550.9772421472648819No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG49000.9472772545198658No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC43290.8368904560849998No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG43150.8341839496435145No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT38680.7477690654046614No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT27500.53163519386319No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG24210.4680322924882847No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC24020.4643591766034117No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA23080.4461869190677245No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA21660.4187352108755162No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG19170.37059806059481276No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA18900.36537836960051967No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTCGTATCTCGTAT14530.2808967042484418TruSeq Adapter, Index 11 (97% over 37bp)
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA14200.2745170819220836No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA13630.2634977342674647No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13040.2520917428354908No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGCATCTCGTAT13040.2520917428354908TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT11950.23101965696964072No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGAATCTCGTAT9110.17611624058522402TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTTCGTATCTCGTAT8220.1589105924929244TruSeq Adapter, Index 21 (97% over 37bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA7660.14808456672698309No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA7370.1424782319553349No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATATCGTAT6650.1285590559705532TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA40550.0243.79161
GGGGGGA41100.0242.913621
GGGTTAT41100.0240.827262
GGTTATT42000.0235.666673
TATTAGT42450.0233.168446
TTATTAG42800.0231.54795
ATTAGTA43300.0228.591227
GTTATTA44450.0222.677174
GGGGGAG44950.0222.380422
TAGTAGA45550.0217.030739
GGAGCGA31100.0214.670435
GCGAAAG31600.0211.273748
GAGCGAA31950.0209.342716
AGCGAAA32150.0208.421467
CGAAAGC32100.0207.601269
TTAGTAG49250.0200.974628
GGGGAGC50650.0197.35443
GGGAGCG35600.0187.535134
GAGCAAA21500.0153.83726
AGCAAAA26550.0124.1148767
Produced by FastQC (version 0.11.2)