FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a5.341000000085f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a5.341000000085f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340713
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT5481016.086853157936446TruSeq Adapter, Index 13 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG189515.562159354060456No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA122853.60567398367541No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG83812.4598415675363134No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA80732.369442903558127No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT57921.6999644862391514No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC56281.6518301326923244No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA54581.6019347662108578No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT48441.4217244425660307No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG43081.2644072870715235No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA38761.1376143557774432No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG29810.8749299263603091No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA24820.7284723506294154No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG23060.6768159712133085No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA20110.590232835260175No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13990.4106095159268945No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA12530.3677582011839877No Hit
GGGGGGAGCGAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA7040.20662551766442724No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC6100.17903631502173384No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC5500.1614261856753338No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT5370.15761065765028043TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT5100.14968609944440042No Hit
CGGGTTATTAGTAGAAACAAGGGGAAAGAAAAGAGTATTGTTTTCTAGAG5000.14675107788666708No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT4990.14645757573089374No Hit
CGGGTTATTAGTAGAAACAAGGTGGGGGCACCTGGGTGACTCAGTGGGTT4740.1391200218365604No Hit
CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC4740.1391200218365604No Hit
CGGGTTATTAGTAGAAACAAGGAAGAACATTCAAGATAGAGGGAAGTATG4660.13677200459037372No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA4650.13647850243460038No Hit
GGGGGGAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA3670.10771529116881363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC100.0014757657244.99898245
CGGTAAT100.0014757657244.99898245
CGGGTTA55700.0243.899351
GTTATTA56450.0240.441864
TTATTAG56450.0240.007865
ATTAGTA56600.0239.371787
GGTTATT56800.0238.960273
TATTAGT56750.0238.739096
GGGGGGA58200.0238.684591
TAGTAGA56900.0238.109739
GGGTTAT57300.0237.08892
GGAGCGA41000.0230.358785
GGGAGCG41400.0228.4294
GCGAAAG41350.0228.408978
GAGCGAA41600.0227.330786
CGAAAGC41600.0227.03639
AGCGAAA41750.0226.807427
GGGGGAG62800.0221.006242
GGGGAGC64250.0215.637223
TTAGTAG64800.0209.08098
Produced by FastQC (version 0.11.2)