FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a4.3410000000857c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a4.3410000000857c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268839
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT7575928.180063160478948TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG32511.2092739520679663No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG26320.979024620683755No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA23130.8603662415051387No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC22400.8332124431351106No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC17330.6446237339076548No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG14630.5441918769226191No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG14390.5352646007461715No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATATCGTAT14240.5296850531358918TruSeq Adapter, Index 7 (97% over 38bp)
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10830.4028433374621986No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC10300.38312893590587677No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC9860.3667622629157228No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA8000.2975758725482538No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC7300.2715379837002816No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA6880.25591525039149826No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA6270.2332250901096939No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5900.21946220600433716No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATTTCGTAT5680.2112788695092602TruSeq Adapter, Index 7 (97% over 38bp)
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT5580.20755917110240701No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA5410.20123568381075663No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5220.1941682568377356No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA4430.16478263942359553No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTTCCATCTCGTAT4310.16031900133537172TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG4240.1577152124505745No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA4210.15659930292851856No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG3720.138372780734938TruSeq Adapter, Index 7 (97% over 37bp)
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA3680.13688490137219675No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT3220.11977428870067214No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3050.11345080140902175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA20350.0239.58231
GGGTTAT20850.0233.24942
GGTTATT21050.0231.033253
TATTAGT21150.0229.94096
TTATTAG21200.0229.398595
GGGGGGA40600.0229.310351
GTTATTA21400.0227.254684
ATTAGTA22300.0218.632287
TAGTAGA23100.0211.060619
GGAGCGA27100.0203.865315
GAGCGAA27450.0201.265936
GGGAGCG27800.0198.732014
AGCGAAA27700.0198.564997
GCGAAAG27900.0197.141598
GGGGGAG47600.0196.102952
TTAGTAG25300.0192.70758
CGAAAGC28600.0192.316449
GGGGAGC48800.0190.527663
GGAGCAA22900.0164.759835
GAGCAAA23000.0164.043496
Produced by FastQC (version 0.11.2)