FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a3.341000000084f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a3.341000000084f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323241
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT15246047.16604638644231TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG18050.5584068852651737No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG13450.4160982053637997No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11500.35577169975343476No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10320.31926642969177793No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC9540.29513582744763195No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT9220.28523609319362336No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA8590.2657459913810439No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT7910.24470905609127552TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA7480.2314062881874515No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT7450.23047818810113815TruSeq Adapter, Index 11 (97% over 37bp)
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG6140.18995115099879037No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATATCGTAT6090.18840431752160153TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA5680.175720283008653No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5390.1667486488409577No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT5060.15653954789151128No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATTTCGTAT5010.15499271441432244TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA4960.15344588093713357No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC4860.1503522139827559No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4500.1392150129469962No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4390.13581197929718075No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTCATTATCTCGTAT4280.13240894564736527TruSeq Adapter, Index 11 (97% over 37bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4220.13055274547473866No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC4140.12807781191123652No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT4100.12684034512948544No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC3850.11910617774354121TruSeq Adapter, Index 2 (97% over 34bp)
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3850.11910617774354121No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC3730.11539377739828796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA11150.0237.309421
GGGTTAT11450.0231.09172
ATTAGTA12500.0212.667
TTATTAG12500.0212.665
GGGGGGA31550.0210.443761
GTTATTA12700.0209.311024
TATTAGT12750.0208.49026
TTAGTAG12750.0208.49028
TAGTAGA13000.0204.480779
GGTTATT13400.0198.376863
GGAGCGA22500.0193.822225
GGGGGAG35350.0187.821782
GAGCGAA23350.0186.241976
GGGAGCG23550.0184.140124
AGCGAAA23500.0184.010657
GCGAAAG23700.0182.45788
CGAAAGC23900.0180.930959
GGGGAGC36750.0180.03
GGAGCAA14300.0160.19235
GAGCAAA15400.0147.954566
Produced by FastQC (version 0.11.2)