FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a2.3410000000847d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a2.3410000000847d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390115
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT7979620.454481370877815TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT20520.5259987439601144No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC19290.494469579482973No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG16810.43089858118759855No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG16340.4188508516719429No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG16060.4116734808966587No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG13860.3552798533765685No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA12280.3147789754303218No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA12170.3119592940543173No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA11860.30401291926739554No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC10660.27325275880189176No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG9440.24197992899529625No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT8870.22736885277418198No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT7800.19994104302577445TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA7170.183791958781385No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC6400.16405418914935338No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG5610.1438037501762301No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC5330.13662637940094588No Hit
CGGGTTATTAGTAGAAACAAGGCAGGGCATCGGTCTTGCTTGCTGTTTGC5230.13406303269548722No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5140.13175602066057443No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4410.11304358971072632No Hit
CGGGTTATTAGTAGAAACAAGGTTAATTTCAAATTTGTGTCTTCAAAGAT4190.1074042269587173No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATTTCGTAT4120.10560988426489624TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT4020.1030465375594376No Hit
CGGGTTATTAGTAGAAACAAGGGTTATGAGTAGAGGGTGTTTCAAGAAGG3980.10202119887725415No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC3960.1015085295361624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA17950.0240.222841
GGGTTAT18800.0229.36172
TTATTAG19000.0226.947365
TATTAGT19200.0224.583316
GTTATTA19500.0221.12824
TAGTAGA19800.0217.15919
ATTAGTA20400.0211.372567
GGGGGGA60050.0208.07661
TTAGTAG20950.0205.238688
GGAGCGA38750.0198.529045
AGCGAAA38950.0198.138647
GCGAAAG39600.0194.886378
GAGCGAA39600.0194.886376
GGTTATT22300.0193.363243
GGGAGCG40200.0191.672884
CGAAAGC40300.0191.197279
GGGGAGC65000.0190.534623
GGGGGAG68200.0183.031522
GAGCAAA27200.0167.536766
GGAGCAA28500.0164.1935
Produced by FastQC (version 0.11.2)