FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n01 flu mrt-pcr updated a1.341000000083f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n01 flu mrt-pcr updated a1.341000000083f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258749
Sequences flagged as poor quality0
Sequence length251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT6698825.88918218041422TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC45521.7592338521115056No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT37371.4442567893982199TruSeq Adapter, Index 27 (97% over 39bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG34831.3460921588102757No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG32161.2429033542158618No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA31131.2030964370876795No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG30201.1671542691952432No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC29651.1458981483986412No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG15690.6063791550885221No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA15510.5994226064641796No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA15420.5959443321520084No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13810.5337218694565004No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG13590.5252194211378595No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG11960.4622240085952023No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11830.45719983458873276No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA11040.4266683156263406No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8590.33198195935056757No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC7100.27439719573795457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATTTCGTAT7080.27362424589080536TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA6430.24850337585845741No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA5330.2059911342652532No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5190.20058048533520903No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA4060.15690881897128106No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3770.14570104618761812No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3100.11980722630812099No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT2610.10086995505296638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT100.0014755423245.000021
CGCGAGG100.0014755423245.00002245
CGGGTTA22250.0244.449451
GGGGGGA18050.0244.32131
GGGTTAT22450.0242.271712
GGTTATT23200.0233.911643
TATTAGT23350.0231.884376
ATTAGTA23550.0229.915077
TTATTAG23600.0229.427955
GGGGGAG19700.0223.857862
GTTATTA24500.0221.000024
GAGCGAA12700.0220.885826
GGAGCGA12800.0219.160175
TAGTAGA24900.0216.957829
GCGAAAG13100.0216.011448
CGAAAGC13150.0215.190119
AGCGAAA13150.0214.258567
TTAGTAG26050.0207.850288
GGGGAGC22800.0193.421053
GGGAGCG15250.0183.950824
Produced by FastQC (version 0.11.2)