FastQCFastQC Report
Wed 8 Apr 2015
000000000-AE3MC l01n03.333000000045ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE3MC l01n03.333000000045ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5634969
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG324390.575673087110151No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG246460.43737596426883624No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG96720.17164247043772557No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGCGGTTTGGTA89030.15799554531710822No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA76930.13652249018583776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTC227450.01216.9655
GATACCC174300.01140.83548
TCGATAC130400.01132.55976
TTGATAC45700.01121.02586
TTGGGTC9750.01076.38327
CGATACC140250.01057.44877
TTGCTCC278750.01026.68596
TGCTCCC279050.01025.33467
TTCGATA137950.01025.0295
ATACCCG135650.0972.168339
GCTCCCC361800.0932.422678
TTTGGGT10450.0931.602666
CTCCCCA332100.0901.87979
GTTTGGG16000.0858.73865
ATACCCA80550.0832.30339
TTTGATC23400.0814.368845
GGGTCTC13500.0792.7319
GTCGATA7600.0790.37575
TTTGCTA77350.0742.66155
TGATACC71550.0712.155157
Produced by FastQC (version 0.11.2)