000000000-AE3MC l01n02.332000000045e1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AE3MC l01n02.332000000045e1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5634969
Sequences flagged as poor quality	0
Sequence length	12
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGAGACAAGGGA	325380	5.7742997343907305	No Hit
AATCAGTCTCGT	297687	5.2828507131095135	No Hit
AATCCGTACAGC	286771	5.089131812437655	No Hit
ACACCTGGTGAT	173037	3.0707711080575595	No Hit
AGGCTACACGAC	134163	2.3809004095674706	No Hit
TATCGTTGACCA	132451	2.3505186985057063	No Hit
CTAACCTCCGCT	113948	2.0221584182628156	No Hit
TTACTGTGCGAT	112161	1.9904457327094436	No Hit
CTAGATTTGCCA	108576	1.9268251520105966	No Hit
ATTGGGCTAGGC	86182	1.5294139151431	No Hit
ACCACATACATC	77938	1.3831131990255847	No Hit
ATGTGGGACCCA	74098	1.314967305055272	No Hit
CTACCGGATCAA	70640	1.2536005078288808	No Hit
GACTCTTGGCAA	62225	1.1042651698704997	No Hit
GTCTACACACAT	57509	1.0205734938382092	No Hit
ACAAGGAGGTGA	52700	0.9352314094363252	No Hit
CCTAGTACTGAT	49313	0.8751246013953227	No Hit
ACTATTGTCACG	48719	0.8645832834217899	No Hit
AATGGAGCATGA	45004	0.7986556802708232	No Hit
ATGGTTGTTGGC	41897	0.7435178436651559	No Hit
TGGCAAGACTCT	40266	0.7145735850543278	No Hit
ACACTAGATCCG	39947	0.7089125068833564	No Hit
TGCAATGTTGCT	38677	0.6863746721587998	No Hit
CTCGAGAGTACG	37753	0.6699770664221932	No Hit
CGGGTTTGACGA	35604	0.6318402106559947	No Hit
CCAAGTCTTACA	32695	0.5802161467081718	No Hit
GGTCACTGACAG	31662	0.5618841913770954	No Hit
GCAGGATAGATA	31337	0.5561166352467956	No Hit
AAGATGGATCAG	31075	0.551467097689446	No Hit
CCATACATAGCT	30677	0.544404059720648	No Hit
AATCTTGCTGCA	28886	0.5126203888610568	No Hit
GTCCGAAACACT	28716	0.509603513346746	No Hit
ACGATGCGACCA	28058	0.497926430473708	No Hit
CGTCCGAAATAC	27203	0.48275332126938053	No Hit
CCTCTCGTGATC	26566	0.4714489112539927	No Hit
CACATCTAACAC	24585	0.43629343834899537	No Hit
TCGTCGATAATC	24314	0.4314841838526529	No Hit
TAACACCACATC	24211	0.42965631221751177	No Hit
TGACCTCCAAGA	23965	0.42529071588503864	No Hit
AGCAAACACCCG	23070	0.4094077536185204	No Hit
CCATGCGATAAC	22763	0.4039596313662063	No Hit
TACTAATCTGCG	22462	0.3986179870732208	No Hit
ACTCACGGTATG	22341	0.39647068156009374	No Hit
GATTGGTTGCAC	21410	0.3799488515376038	No Hit
TCTGTTGCTCTC	21283	0.3776950680651482	No Hit
GTATTACGATCC	21023	0.37308102316090824	No Hit
TGGCACCGATTA	20894	0.37079174703534307	No Hit
AACGAGAACTGA	19951	0.3540569610941959	No Hit
TAAGGTAAGGTG	19461	0.3453612610823591	No Hit
CTCTACCTCTAC	18214	0.3232315918685622	No Hit
CTTACACCAAGT	17945	0.3184578300253293	No Hit
CCATAGGGTTCA	17756	0.315103774306478	No Hit
AGCCGGCACATA	17539	0.31125282144409316	No Hit
GCATGGCTCTAA	16811	0.2983334957122213	No Hit
GTGGGATGTTTC	16640	0.29529887387135584	No Hit
CGACTGTCTTAA	16277	0.2888569573319747	No Hit
AGCCTAAGCACG	15601	0.27686044058095083	No Hit
TCGGAGTGTTGT	15548	0.2759198852735481	No Hit
TCAAGCTCAAGC	15305	0.2716075279207392	No Hit
GTACCTAATTGC	15265	0.27089767485854843	No Hit
GCCTGAATTTAC	15249	0.27061373363367214	No Hit
TACTTCGCTCGC	14039	0.2491406785024017	No Hit
CGTGACAATGTC	14014	0.2486970203385325	No Hit
CAGGAAGGTTAA	13391	0.2376410588949114	No Hit
CGCCAAATAACC	12822	0.22754339908524784	No Hit
ATTAGTTCGCGT	12819	0.22749016010558354	No Hit
TGTAATTGTCGC	11051	0.196114654756752	No Hit
TAACGCTTGGGT	10983	0.1949079045510277	No Hit
TAATCCACAGCG	10596	0.1880400761743321	No Hit
TCTACGGAGAGC	9908	0.17583060350465105	No Hit
GCTTCGGTAGAT	9550	0.16947741859804374	No Hit
CGGACTACAACT	9299	0.16502309063279674	No Hit
AATGTCCGTGAC	9165	0.1626450828744577	No Hit
ATCGCTCGAGGA	8813	0.156398375927179	No Hit
TCAACAGCATCG	8563	0.15196179428848677	No Hit
AGCTTGACAGCT	8470	0.15031138591889326	No Hit
GACTCGAATCGT	8417	0.1493708306114905	No Hit
ACGGCATGGCAT	7598	0.1348365891631347	No Hit
TTATGCAGTCGT	7299	0.12953043752325877	No Hit
GAATTCGTATCT	7117	0.1263006060902908	No Hit
TTGATGCTATGC	6498	0.11531562995288883	No Hit
ATACCTTCGGTA	6494	0.11524464464666975	No Hit
ATGATGACCCGT	6467	0.11476549382969099	No Hit
GCTCAGTGCAGA	6395	0.11348775831774763	No Hit
TATCAGGTGTGC	5735	0.10177518279160011	No Hit

Adapter Content

Can't analyse adapters as read length is too short

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGAGC	1055	0.0	6.0000005	6
GGGTCCC	55	0.009158245	6.0000005	5
GGTCCCA	55	0.009158245	6.0000005	6
GGTCAGC	655	0.0	6.0000005	1
TTTATGC	55	0.009158245	6.0000005	1
TGCTAGG	145	1.9463187E-10	6.0000005	5
CCCGGTG	55	0.009158245	6.0000005	4
ACCGGGT	65	0.0012615051	6.0	3
CGTTGTC	90	9.146124E-6	6.0	4
TGTGGGC	130	3.6416168E-9	6.0	2
AGCCAAG	245	0.0	6.0	3
CCCAGTC	100	1.2855253E-6	6.0	1
GCAGTCG	800	0.0	6.0	5
AGGCGGT	75	1.7505152E-4	6.0	4
TGCGGGA	65	0.0012615051	6.0	2
GCGCATG	100	1.2855253E-6	6.0	5
TCGGCGT	100	1.2855253E-6	6.0	1
GCACCGA	2350	0.0	6.0	3
GGCCACT	95	3.4272234E-6	6.0	1
GCACATC	100	1.2855253E-6	6.0	6

Summary