##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE2TW l01n03.33300000008a65.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16299268 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.637029282542013 32.0 29.0 32.0 18.0 33.0 2 31.070294015657634 32.0 32.0 33.0 29.0 33.0 3 30.557295947278124 32.0 32.0 33.0 18.0 33.0 4 31.308962157073555 32.0 32.0 33.0 29.0 33.0 5 31.712747529520957 32.0 32.0 33.0 31.0 33.0 6 36.18066075114539 37.0 37.0 37.0 34.0 37.0 7 36.457837677127586 37.0 37.0 37.0 37.0 37.0 8 36.54242251860636 37.0 37.0 37.0 37.0 37.0 9 36.57714628656943 37.0 37.0 37.0 37.0 37.0 10-11 36.388770465029474 37.0 37.0 37.0 37.0 37.0 12-13 36.94247358224922 37.5 37.5 37.5 37.0 37.5 14-15 37.05946052301245 38.0 37.5 38.0 36.0 38.0 16-17 36.88435864113652 38.0 37.5 38.0 35.0 38.0 18-19 37.54304085925822 38.0 38.0 38.0 37.5 38.0 20-21 36.85279409480229 38.0 38.0 38.0 36.0 38.0 22-23 36.82737884302534 38.5 38.0 38.5 34.0 38.5 24-25 36.88701247197113 38.5 38.0 39.0 34.0 39.0 26-27 36.080640124452216 38.0 36.5 39.0 32.0 39.0 28-29 38.02720741814908 39.0 38.0 39.0 37.0 39.0 30-31 36.12378994565891 38.0 37.0 39.0 31.5 39.0 32-33 35.3072388281486 37.5 35.5 39.0 26.0 39.0 34-35 35.82699744552946 38.0 36.5 39.0 32.0 39.0 36-37 36.06033780167306 38.5 37.0 39.0 32.0 39.0 38-39 36.55968688287106 38.5 37.0 39.0 33.0 39.0 40-41 35.85299063737095 38.0 36.5 38.5 32.0 39.0 42-43 36.890916481648134 38.0 36.5 39.0 34.5 39.0 44-45 35.76267774724607 38.0 36.5 39.0 32.5 39.0 46-47 35.737569380416346 38.5 36.5 39.0 26.0 39.0 48-49 35.701609851436274 38.0 36.5 39.0 26.0 39.0 50-51 35.70901432506049 38.0 36.5 39.0 26.0 39.0 52-53 36.38311699028447 38.0 37.0 39.0 32.5 39.0 54-55 36.13987824483897 38.0 36.5 39.0 32.5 39.0 56-57 36.059510954725084 38.5 36.5 39.0 32.0 39.0 58-59 35.334807949657616 38.0 36.0 39.0 25.0 39.0 60-61 34.50116459217678 38.0 35.0 38.0 18.0 39.0 62-63 35.45162862528551 38.0 36.0 39.0 29.5 39.0 64-65 35.797594008516214 38.0 37.0 39.0 26.0 39.0 66-67 35.33187987337837 38.0 36.0 39.0 20.0 39.0 68-69 34.51597133073706 37.5 35.0 39.0 19.0 39.0 70-71 34.830740282324335 38.0 35.5 39.0 19.0 39.0 72-73 34.594565596442735 38.0 35.5 39.0 18.0 39.0 74-75 35.28243096561146 38.0 36.0 39.0 24.5 39.0 76-77 35.34089503283215 38.0 36.0 39.0 20.0 39.0 78-79 35.53131468848785 38.0 37.0 39.0 20.0 39.0 80-81 35.259057001823635 38.0 36.0 39.0 19.5 39.0 82-83 35.254817394253536 38.0 36.0 39.0 19.0 39.0 84-85 34.79040574092039 38.0 35.5 39.0 18.0 39.0 86-87 35.19703706939477 38.0 36.0 39.0 19.0 39.0 88-89 35.9624699710441 38.0 37.0 39.0 31.0 39.0 90-91 35.23142573028433 38.0 36.0 39.0 28.0 39.0 92-93 35.024610031567065 38.0 36.0 39.0 18.0 39.0 94-95 35.29604047249238 38.0 36.0 39.0 24.0 39.0 96-97 35.246635493078585 38.0 36.0 39.0 19.0 39.0 98-99 34.944298265419036 38.0 36.0 39.0 18.5 39.0 100-101 34.297523851991386 37.5 34.5 38.5 18.5 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0130020151363759 1101 2 0.5039042004081153 1101 3 0.5224612421244501 1101 4 0.4134532374799704 1101 5 0.3249236783618592 1101 6 0.1478711011263556 1101 7 0.07498149986360403 1101 8 0.12926374660040096 1101 9 0.11333617544130448 1101 10-11 0.14047264940184334 1101 12-13 0.14298289465528313 1101 14-15 0.23756987588939893 1101 16-17 0.21674372200597247 1101 18-19 0.10053965865576231 1101 20-21 0.2936130933520502 1101 22-23 0.3166418545188989 1101 24-25 0.3485378578977105 1101 26-27 0.5999163082916041 1101 28-29 0.25384342874267674 1101 30-31 0.4481039134376559 1101 32-33 0.6480397352209337 1101 34-35 0.6317988303904372 1101 36-37 0.42292055314494803 1101 38-39 0.586506331468378 1101 40-41 0.477667673725918 1101 42-43 0.3057706509967346 1101 44-45 0.5228457003456342 1101 46-47 1.063000631669162 1101 48-49 0.7864071114553255 1101 50-51 0.6602360187137393 1101 52-53 0.603392167111366 1101 54-55 0.3906063396124395 1101 56-57 0.27487966688860865 1101 58-59 0.3175871865479323 1101 60-61 1.1697276485299426 1101 62-63 0.7094451156249022 1101 64-65 0.5298115589981478 1101 66-67 0.6911323413756278 1101 68-69 0.6318226390142243 1101 70-71 0.630230397809342 1101 72-73 0.6634570136719944 1101 74-75 0.5815022192738795 1101 76-77 0.64208712738359 1101 78-79 0.5555874602792059 1101 80-81 0.7296667454457904 1101 82-83 0.687572817277669 1101 84-85 1.1326533496113171 1101 86-87 0.7090894660635811 1101 88-89 0.5515645434982801 1101 90-91 0.7939655461390842 1101 92-93 0.5690704481443731 1101 94-95 0.6713974827603479 1101 96-97 0.6691466469760883 1101 98-99 0.5973103304474421 1101 100-101 0.4945106946403186 1102 1 0.9509951344123877 1102 2 0.6008667263549725 1102 3 0.3781461850950727 1102 4 0.3644636196398068 1102 5 0.2996316462032418 1102 6 0.09410535015886268 1102 7 0.046534149186769014 1102 8 0.121803802905184 1102 9 0.10464010554221659 1102 10-11 0.20069232785741065 1102 12-13 0.1807666943780859 1102 14-15 0.28234041287281997 1102 16-17 0.2024285144705047 1102 18-19 0.1317850595018939 1102 20-21 0.22190702086973602 1102 22-23 0.3553810388114158 1102 24-25 0.5526793028508692 1102 26-27 0.6379571385590168 1102 28-29 0.2098579711853148 1102 30-31 0.4541304748934678 1102 32-33 0.56959041094116 1102 34-35 0.7585832476270369 1102 36-37 0.4341464823120873 1102 38-39 0.575175710935774 1102 40-41 0.2925911647351427 1102 42-43 0.3319816899731194 1102 44-45 0.4639526999473347 1102 46-47 1.1252430228385037 1102 48-49 0.6833350620820582 1102 50-51 0.7259565267781127 1102 52-53 0.5693700152288557 1102 54-55 0.4096447782863919 1102 56-57 0.2635239464463126 1102 58-59 0.09884112494238906 1102 60-61 0.8428787803894409 1102 62-63 0.755874445419316 1102 64-65 0.5207807250833199 1102 66-67 0.7082330172254743 1102 68-69 0.4562695158209422 1102 70-71 0.575261960471181 1102 72-73 0.6978457168241476 1102 74-75 0.623960888071089 1102 76-77 0.5591310318437621 1102 78-79 0.6349060848413686 1102 80-81 0.5937317569879994 1102 82-83 0.6870433072173583 1102 84-85 1.1452860680292218 1102 86-87 0.7723273440824201 1102 88-89 -0.3672142634525599 1102 90-91 0.10058868529861087 1102 92-93 0.5580099519685433 1102 94-95 0.6257753017949241 1102 96-97 0.6536013726941903 1102 98-99 0.5341214314867386 1102 100-101 0.486515916685093 1103 1 0.820234274843056 1103 2 0.32767071873780296 1103 3 0.4906092732708096 1103 4 0.3576683997236181 1103 5 0.25307228259366354 1103 6 0.12909643341644994 1103 7 0.06904643196720173 1103 8 0.14280303378731674 1103 9 0.1303736525141943 1103 10-11 0.22793935879944627 1103 12-13 0.1684618718737525 1103 14-15 0.22708573446792002 1103 16-17 0.31512925605657927 1103 18-19 0.17821582695233928 1103 20-21 0.19922266559454727 1103 22-23 0.3839262625846587 1103 24-25 0.23515574822098984 1103 26-27 0.5293944813524121 1103 28-29 0.19161804400312832 1103 30-31 0.3927783910185383 1103 32-33 0.38889079621174005 1103 34-35 0.7261579054292824 1103 36-37 0.6329009863156045 1103 38-39 0.4570164494939988 1103 40-41 0.27348354829788946 1103 42-43 0.24889037570260086 1103 44-45 0.3171464492964162 1103 46-47 0.7074257599013904 1103 48-49 0.648364133066444 1103 50-51 0.6100193106803502 1103 52-53 0.3615845307504486 1103 54-55 0.2384450133992715 1103 56-57 0.18459855893127042 1103 58-59 0.1889550698640221 1103 60-61 0.7983688661510726 1103 62-63 0.6288989816195496 1103 64-65 0.5368115302857106 1103 66-67 0.5805870583272466 1103 68-69 0.5015650346741296 1103 70-71 0.6534469157264056 1103 72-73 0.532290098163898 1103 74-75 0.4452524476664408 1103 76-77 0.5960810344764838 1103 78-79 0.3631716431006069 1103 80-81 -0.42999953852200434 1103 82-83 0.19624796862275673 1103 84-85 1.0398709671469248 1103 86-87 0.5769202338091333 1103 88-89 -0.3995519991936689 1103 90-91 0.5099411682913697 1103 92-93 0.43879396183718455 1103 94-95 0.5578767142726164 1103 96-97 0.5766832413880465 1103 98-99 0.48912522863187036 1103 100-101 0.4553646274028722 2112 1 0.6310870207504884 2112 2 0.44602547817284943 2112 3 0.61723848954367 2112 4 0.40541428785939715 2112 5 0.30736178774737866 2112 6 0.11520622911012879 2112 7 0.037688974386590246 2112 8 0.12153178883998095 2112 9 0.1052975415864168 2112 10-11 0.16212482092490177 2112 12-13 -0.14147644273015914 2112 14-15 0.0904284761480767 2112 16-17 0.04306467843277062 2112 18-19 0.1446651751112782 2112 20-21 -0.8841761065929958 2112 22-23 0.45174303340742483 2112 24-25 0.5130947699468678 2112 26-27 0.5937544706546518 2112 28-29 0.1304840986226381 2112 30-31 0.5580853364474976 2112 32-33 0.6419271472720922 2112 34-35 0.6008271068729485 2112 36-37 0.7158479287730444 2112 38-39 0.7152309572820315 2112 40-41 0.7872033338731583 2112 42-43 0.34719432638425474 2112 44-45 0.4185012542001516 2112 46-47 1.0460539651834537 2112 48-49 0.8842936227168963 2112 50-51 0.7976425397853788 2112 52-53 0.6586494127366507 2112 54-55 0.5412278936930619 2112 56-57 0.5202477429234875 2112 58-59 0.47236762285204037 2112 60-61 0.9892760855466136 2112 62-63 0.8141154709924123 2112 64-65 0.7039688387630676 2112 66-67 0.8043698564831487 2112 68-69 0.8021221079834717 2112 70-71 0.8899339602603717 2112 72-73 0.9203444749282284 2112 74-75 0.734570313683804 2112 76-77 0.7439804002270947 2112 78-79 0.6505627199680717 2112 80-81 0.9922730658605374 2112 82-83 0.7397084810621379 2112 84-85 1.3288584444983087 2112 86-87 0.9189791336994233 2112 88-89 0.17647870559414258 2112 90-91 0.8431555510440418 2112 92-93 0.7840083178999748 2112 94-95 0.7323734084011377 2112 96-97 0.7821409071436065 2112 98-99 0.7385576806732104 2112 100-101 0.4770062012353691 1113 1 0.8656913791427279 1113 2 0.3469400247507828 1113 3 0.5470473109783711 1113 4 0.31798448350118136 1113 5 0.27731459293862315 1113 6 0.04970195213456208 1113 7 0.05286057301911029 1113 8 0.0929464997012559 1113 9 0.09661922881783624 1113 10-11 0.17286015390847354 1113 12-13 0.1584818458356878 1113 14-15 0.11715113203804606 1113 16-17 0.1461353936523011 1113 18-19 0.12023971938599942 1113 20-21 0.3960591333524661 1113 22-23 0.5242829943339231 1113 24-25 0.3928418821835393 1113 26-27 0.5282165034264708 1113 28-29 0.21998119928196047 1113 30-31 0.46553783728754894 1113 32-33 0.5162043627321395 1113 34-35 0.5703122533842944 1113 36-37 0.5271292045450195 1113 38-39 0.54686720327188 1113 40-41 0.5528622990228982 1113 42-43 0.21549945538404103 1113 44-45 0.23546722647876805 1113 46-47 0.8096162120429042 1113 48-49 0.6933317009951807 1113 50-51 0.5751509722217989 1113 52-53 0.46911795739478634 1113 54-55 0.40431235343760363 1113 56-57 0.3467369756932044 1113 58-59 0.13659021163602603 1113 60-61 1.0247951166652882 1113 62-63 0.6306934276631111 1113 64-65 0.5770487479152138 1113 66-67 0.6879189410359743 1113 68-69 0.7442116572397381 1113 70-71 0.7533462796183059 1113 72-73 0.6404164367028784 1113 74-75 0.46124741706091044 1113 76-77 0.6099236958707621 1113 78-79 0.5399792067136389 1113 80-81 0.8389507822047051 1113 82-83 0.7041232481383446 1113 84-85 0.9590963249614077 1113 86-87 0.3081300731771748 1113 88-89 0.4550646401688354 1113 90-91 0.5826459299948041 1113 92-93 0.6265015613751501 1113 94-95 0.5351904017003406 1113 96-97 0.5019846117944269 1113 98-99 0.5462482982897754 1113 100-101 0.4843194208628887 2113 1 0.7336617619886674 2113 2 0.32581074182974845 2113 3 0.4350129692802476 2113 4 0.2540220187358635 2113 5 0.23636905182128132 2113 6 0.04883925940958278 2113 7 0.031296702453026626 2113 8 0.10929806749739157 2113 9 0.08043042098713471 2113 10-11 0.16836324359197619 2113 12-13 0.14370819957240855 2113 14-15 0.22159033597354494 2113 16-17 0.1383040025581863 2113 18-19 0.122241742870429 2113 20-21 0.19456539165438613 2113 22-23 0.3655728478076057 2113 24-25 0.2849174998342008 2113 26-27 0.5491121080272947 2113 28-29 0.17381355797524378 2113 30-31 0.3751051504065046 2113 32-33 0.5280037306971721 2113 34-35 0.5869059348112984 2113 36-37 0.3609510453657805 2113 38-39 0.35361504612064465 2113 40-41 0.4053124796711174 2113 42-43 0.23244305849769376 2113 44-45 0.2530813075233169 2113 46-47 0.9524579584641018 2113 48-49 0.6386470458841487 2113 50-51 0.5371020753910116 2113 52-53 0.3867305463767252 2113 54-55 0.3306023942098264 2113 56-57 0.1613701274157009 2113 58-59 0.36592312295629625 2113 60-61 1.1412471229655594 2113 62-63 0.16528670473427098 2113 64-65 0.3974185042708811 2113 66-67 0.4008592954686421 2113 68-69 0.5297618786370677 2113 70-71 0.48799630251218673 2113 72-73 0.5730924669494186 2113 74-75 0.32409770454355424 2113 76-77 0.575272006267312 2113 78-79 0.4993644098090826 2113 80-81 0.6770516426944013 2113 82-83 0.6521389485700126 2113 84-85 1.01576864331372 2113 86-87 0.6212376637631181 2113 88-89 0.6606398428198119 2113 90-91 0.6671046463963108 2113 92-93 0.3854466768214593 2113 94-95 0.4249800817922136 2113 96-97 0.48168259439376016 2113 98-99 0.32658512021676245 2113 100-101 0.4178312351384079 1112 1 0.7258387508542583 1112 2 0.25387443903234796 1112 3 0.2818421340332442 1112 4 0.20701898291449794 1112 5 0.20219261519613596 1112 6 0.09374142607470048 1112 7 0.0630333388418336 1112 8 0.11211945097952736 1112 9 0.09931297514351911 1112 10-11 0.18029120714335534 1112 12-13 0.16085654677367955 1112 14-15 0.23478517127146148 1112 16-17 0.15978152997281825 1112 18-19 0.12434840831321026 1112 20-21 0.24602742167653702 1112 22-23 0.23974143663740222 1112 24-25 0.10961822030672863 1112 26-27 0.37457652846371303 1112 28-29 0.08023802386296808 1112 30-31 0.268830219677902 1112 32-33 0.5234544279971374 1112 34-35 0.5323980150893206 1112 36-37 0.299975882424377 1112 38-39 0.4826682225126291 1112 40-41 0.43025478623847135 1112 42-43 0.27678967916511965 1112 44-45 0.24797052164304034 1112 46-47 0.8024411264470999 1112 48-49 0.6477517605980907 1112 50-51 0.4589293338906728 1112 52-53 0.4357574825836181 1112 54-55 0.3403680550237027 1112 56-57 0.18388370161137146 1112 58-59 0.26216710411248556 1112 60-61 0.8454254716416827 1112 62-63 0.10623892279430436 1112 64-65 0.3739558471423905 1112 66-67 0.48567816958161814 1112 68-69 0.5111733757999488 1112 70-71 0.45118238922408693 1112 72-73 0.5396688529814355 1112 74-75 0.3422742283489484 1112 76-77 0.4424064584704581 1112 78-79 0.40522899518057187 1112 80-81 0.7319337565990693 1112 82-83 0.5151890859137325 1112 84-85 0.9314009584457494 1112 86-87 0.5909367822008349 1112 88-89 0.6195386347581078 1112 90-91 0.5851527220291786 1112 92-93 0.38462212034322363 1112 94-95 0.537038127864875 1112 96-97 0.5574527274851633 1112 98-99 0.41262085293677586 1112 100-101 0.1256270261526069 2114 1 0.4938271135316725 2114 2 0.3704714714787656 2114 3 0.3324628799456306 2114 4 0.2507957827280478 2114 5 0.25314016414436225 2114 6 0.12355362601383746 2114 7 0.06279295885205727 2114 8 0.12408212870810331 2114 9 0.08937565736201236 2114 10-11 0.15262983924833406 2114 12-13 0.16374455580852043 2114 14-15 0.25196650461854375 2114 16-17 0.22181358793672246 2114 18-19 0.1227998055945605 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-1.003204078772221 2109 28-29 -0.25066003678910675 2109 30-31 -1.1604133343872505 2109 32-33 -1.1393556140446037 2109 34-35 -1.4962015436410852 2109 36-37 -1.142729276067449 2109 38-39 -1.2685432648874553 2109 40-41 -1.3332086803882888 2109 42-43 -0.5267812398878675 2109 44-45 -1.083155284792788 2109 46-47 -0.8571443262353355 2109 48-49 -1.4654955208156295 2109 50-51 -1.7808234649298669 2109 52-53 -1.165815722047867 2109 54-55 -1.2069595583202002 2109 56-57 -0.8857131432062957 2109 58-59 -1.1377545457873666 2109 60-61 -0.9298564335197952 2109 62-63 -1.0947929073083102 2109 64-65 -1.1640058144331533 2109 66-67 -1.8539865537933693 2109 68-69 -0.9289876510278958 2109 70-71 -1.1588525280467934 2109 72-73 -0.7572183241666437 2109 74-75 -1.0468647451619475 2109 76-77 -0.8056594577664811 2109 78-79 -0.8360381217221899 2109 80-81 -0.39331612794264714 2109 82-83 -0.90499427163207 2109 84-85 -0.8448490759000364 2109 86-87 -1.2662529358074366 2109 88-89 -1.0829857378871637 2109 90-91 -1.2390528481101768 2109 92-93 -1.1679589814970655 2109 94-95 -1.1168002742576775 2109 96-97 -1.3511615528825587 2109 98-99 -1.0177722265418083 2109 100-101 -0.995353798424695 2108 1 -1.9370598395930294 2108 2 -0.6997678096323412 2108 3 -1.328098034367894 2108 4 -0.5028507258626504 2108 5 -0.35946840773501876 2108 6 -0.10138787016321515 2108 7 0.021733231724155644 2108 8 -0.040838651127415915 2108 9 -0.08375846989972047 2108 10-11 -0.09549569778920386 2108 12-13 -0.08449236899101464 2108 14-15 -0.1754232292199518 2108 16-17 -0.1298261112082031 2108 18-19 -0.08642484765979219 2108 20-21 -0.8167255946997756 2108 22-23 -1.2428950760762163 2108 24-25 -1.0033415427547467 2108 26-27 -1.184429397098448 2108 28-29 -0.3907599838342364 2108 30-31 -1.5481181408586266 2108 32-33 -2.4143348114215257 2108 34-35 -2.11685361028686 2108 36-37 -1.0775288968597323 2108 38-39 -1.1895055029504746 2108 40-41 -1.423332387610678 2108 42-43 -0.6627327826974394 2108 44-45 -1.0374011840388775 2108 46-47 -1.5830666970119793 2108 48-49 -2.2671384995940755 2108 50-51 -1.8317384584450096 2108 52-53 -1.9329715576483508 2108 54-55 -0.8225195395441887 2108 56-57 -0.5663783095350965 2108 58-59 -0.5998959865469757 2108 60-61 -0.4047504028597686 2108 62-63 -1.177633540626907 2108 64-65 -1.0154779374069633 2108 66-67 -1.1696918972187618 2108 68-69 -0.5591036288630207 2108 70-71 -1.4848587674735825 2108 72-73 -0.7617482007533596 2108 74-75 -0.8268547118445042 2108 76-77 -0.5282040296344377 2108 78-79 -0.9296486175787422 2108 80-81 -0.9369803569821684 2108 82-83 -0.8755206240897095 2108 84-85 -0.837303251189752 2108 86-87 -1.148155166803754 2108 88-89 -0.43407517798301853 2108 90-91 -0.5911151421808327 2108 92-93 -0.6624678000561772 2108 94-95 -0.8034889538223169 2108 96-97 -1.2067905824934684 2108 98-99 -1.103952923883817 2108 100-101 -1.159281486300415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 9.0 15 58.0 16 324.0 17 2680.0 18 12066.0 19 28108.0 20 33756.0 21 27659.0 22 25370.0 23 32439.0 24 46664.0 25 68770.0 26 105508.0 27 164721.0 28 242775.0 29 337190.0 30 451155.0 31 574495.0 32 661475.0 33 805007.0 34 1042551.0 35 1472915.0 36 2419029.0 37 5538504.0 38 2206040.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 66.13935668767456 26.54660933239456 5.010685142424801 2.3033488375060767 2 93.54792497429946 1.7911479214894803 1.2312822882598164 3.429644815951244 3 4.817148843739486 40.65124274292563 41.93050878113054 12.60109963220434 4 1.2783090184362278 19.089122228625374 77.63746676460971 1.9951019883286778 5 1.0165487186295727 96.5120887637408 1.2359818858122955 1.2353806318173308 6 0.8667014984967423 1.2335400583633571 1.1081724651683744 96.79158597797152 7 0.8380683107977609 1.0612991945405155 1.1533953549325036 96.94723713972921 8 0.8263622636304894 1.063415854012585 96.87969422921324 1.2305276531436873 9 0.8424366051285248 1.0286474214670254 96.97568013483796 1.1532358385664927 10-11 48.63004426425911 1.0831345641759669 49.328041888998996 0.9587792825659189 12-13 0.883671639957797 48.8001546327206 49.27883566788617 1.0373380594354336 14-15 48.60309213282131 1.0860242645873504 1.5409557515391412 48.7699278510522 16-17 0.8971047043339614 48.557984321749906 1.0473415125145498 49.49756946140158 18-19 0.8549801394449167 1.0288223075055367 96.9635567978745 1.1526407551750408 20-21 0.9405453054701597 28.14685604286033 31.022571074970973 39.890027576698536 22-23 15.141658508836104 5.0622610782275625 13.930456876959138 65.8656235359772 24-25 0.8718704690256787 1.2176559468086525 43.653967885366626 54.25650569879904 26-27 39.409794353955036 24.493992613656022 10.616617875109483 25.479595157279455 28-29 0.8313931899086876 1.0913987392378215 51.92666787019723 46.15054020065625 30-31 9.743584803931073 40.02584962711209 27.82960253184376 22.40096303711308 32-33 29.36724520389505 53.699178392551126 11.599971851496644 5.333604552057184 34-35 21.87618180153857 35.46928610536375 32.910502483915224 9.74402960918245 36-37 12.930734067321307 26.6777011090314 28.385995616490263 32.00556920715703 38-39 17.25311836089817 50.92120701371374 5.742313090379274 26.08336153500881 40-41 38.58131543085248 11.413745697046027 11.578010742568317 38.42692812953318 42-43 24.941360094863157 5.452926028731046 68.12483137662475 1.4808824997810486 44-45 55.297434216064175 19.37478419276252 20.164942376553352 5.162839214619945 46-47 5.0697184683385785 48.90658279868765 32.056801569248385 13.966897163725388 48-49 28.398351999611272 37.965711098191655 28.40347186143574 5.23246504076134 50-51 24.55429593525304 19.9659610480667 32.64184931495083 22.837893701729428 52-53 9.961769448787516 1.7846353590848376 59.730056589044366 28.52353860308328 54-55 2.0649823047268137 10.97711565942716 35.02497474119697 51.932927294649055 56-57 14.833003543472016 12.483965537593468 21.257405547292063 51.42562537164246 58-59 17.518486106247224 6.483389315397477 37.7257524693747 38.272372108980605 60-61 18.328323087883454 43.845291089145846 15.653727517088498 22.172658305882205 62-63 34.41520195876281 20.656820294015656 26.488726364889516 18.439251382332017 64-65 13.887329173310114 20.418490572705476 49.51339225786091 16.18078799612351 66-67 16.42185710425769 28.57612992190815 17.433896417925027 37.568116555909135 68-69 31.480505750319587 29.73301009591351 6.697174989698924 32.089309164067984 70-71 11.857538633023275 33.97726511399162 26.761925750285226 27.40327050269988 72-73 13.60089299715791 22.769958135543263 21.269427559568932 42.35972130772989 74-75 23.7033344074102 45.29734402796494 21.272384747584983 9.726936817039881 76-77 12.459719432176938 19.8858476308828 32.984828919459105 34.669604017481156 78-79 16.9694399773045 26.258439949573198 24.275995093767403 32.4961249793549 80-81 30.18446902032656 26.025567528554046 30.9551478017295 12.834815649389899 82-83 29.42964064398475 14.562387096156712 34.17735998941793 21.83061227044061 84-85 26.49563158296434 26.813424995527406 24.169297050640555 22.5216463708677 86-87 5.1895888821510265 41.684979349992894 22.49513597788563 30.630295789970447 88-89 7.819611899135594 17.40760689375744 5.4176788798122715 69.35510232729469 90-91 11.640820311685163 39.35692388148965 9.02305551390406 39.97920029292113 92-93 17.33405144329181 17.07011320999201 32.023300064763646 33.57253528195254 94-95 12.247117477913733 13.117242443034865 17.330216915262696 57.3054231637887 96-97 17.133428936808695 28.043559993000912 26.236859225825356 28.586151844365038 98-99 33.68581644279976 18.211228872364085 30.881061652584645 17.221893032251508 100-101 32.286284267489805 12.55425090255587 40.98020230110947 14.179262528844855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.5 2 1.5 3 1.0 4 0.5 5 1.5 6 2.0 7 1.5 8 1.5 9 1.5 10 2.0 11 4.0 12 7.5 13 12.0 14 12.5 15 16.5 16 40.0 17 67.5 18 93.0 19 390.5 20 507.5 21 330.5 22 650.5 23 925.0 24 1006.5 25 1482.0 26 3171.0 27 7336.0 28 8570.5 29 12075.0 30 18155.5 31 17834.5 32 31908.0 33 141799.0 34 164347.5 35 82210.0 36 90905.5 37 287328.5 38 352348.5 39 216356.5 40 176763.0 41 207782.5 42 338625.5 43 801911.0 44 1320839.0 45 1026491.5 46 766023.0 47 716350.0 48 411756.0 49 546032.5 50 1590107.5 51 2742776.5 52 1990222.5 53 647118.5 54 455351.0 55 412912.5 56 219738.0 57 183559.5 58 148963.5 59 55874.5 60 28967.0 61 19402.0 62 16222.0 63 14116.5 64 9814.5 65 3914.0 66 2066.0 67 1311.5 68 914.5 69 639.0 70 447.0 71 305.0 72 212.5 73 159.5 74 120.0 75 99.0 76 84.5 77 77.0 78 69.0 79 54.0 80 46.5 81 42.5 82 39.5 83 38.5 84 35.5 85 34.0 86 25.5 87 24.5 88 25.0 89 20.0 90 23.5 91 29.0 92 27.0 93 24.0 94 30.0 95 36.0 96 42.0 97 39.5 98 39.5 99 68.5 100 261.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 6.135244846578386E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.067622423289193E-6 12-13 6.135244846578386E-6 14-15 3.067622423289193E-6 16-17 0.0 18-19 3.067622423289193E-6 20-21 0.0 22-23 0.0 24-25 3.067622423289193E-6 26-27 0.0 28-29 3.067622423289193E-6 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 3.067622423289193E-6 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.067622423289193E-6 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6299268E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.954800929012651 #Duplication Level Percentage of deduplicated Percentage of total 1 84.2135658727926 3.3304788854918823 2 5.848994397717204 0.46263216955763586 3 2.1481518595125584 0.2548653890898156 4 1.2308739405829263 0.19471445614859276 5 0.8895766008520353 0.17590491837387723 6 0.5604461505042435 0.13298717740054475 7 0.37113747371580685 0.10274423780898768 8 0.3050418370209352 0.0965103792350496 9 0.24903652154103334 0.0886400880073702 >10 3.012945595589943 3.0607680261961376 >50 0.6693562139906458 1.7502783683691132 >100 0.33610071152795573 2.8494339918794434 >500 0.06990589609877378 1.9072641023849919 >1k 0.06917827069704516 6.009828409321006 >5k 0.012844328930365334 3.3723723819012252 >10k+ 0.012844328925909572 76.21057701883419 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 2478240 15.20460918858442 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 2221685 13.630581446970503 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 1577270 9.676937639162691 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC 584583 3.586559838147333 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT 487492 2.9908827807481906 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAGAAAAA 385291 2.363854622183033 No Hit TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG 350843 2.152507707708101 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA 277295 1.7012727197319537 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 266135 1.632803387244139 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA 263764 1.6182567217129014 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT 261814 1.6062929942620734 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 237080 1.4545438482268038 No Hit AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 220760 1.3544166523306445 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCCAAGGTTTGTTTAA 127997 0.7852929346274937 No Hit GGCCACCTTGTTACGACTTTTGCCCGGTTCAAGCCAACGCGATCAAACCG 124718 0.7651754667755631 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA 114863 0.7047126288125332 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA 103680 0.6361021856932471 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT 95259 0.5844372888402105 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA 87741 0.5383125180836341 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTTAAAAAA 87425 0.5363737807121154 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC 86594 0.5312753922446088 No Hit GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC 86279 0.5293427901179366 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAAGTG 84262 0.516968001262388 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT 80608 0.4945498165929906 No Hit TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC 65490 0.4017971850024185 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC 65007 0.39883386174152113 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCTAT 62938 0.3861400401539505 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA 61629 0.37810900464977937 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA 58270 0.35750071721012255 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA 57147 0.35061083724741504 No Hit TGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGGT 57076 0.350175234863308 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA 54346 0.333426016432149 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC 54181 0.33241370103246354 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG 48988 0.30055337454418196 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 48682 0.298675989621129 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 47990 0.29443040018729677 No Hit AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACATAT 46930 0.2879270406499237 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 40996 0.25152049773032753 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC 40861 0.25069223967603943 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA 35124 0.21549433999121922 No Hit GAAGACCTTGTTACGACTTCTCCTTCTTCTAAATTGCAAGATTCGAACAA 35004 0.21475811060962985 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA 33685 0.20666572265699296 No Hit GGAAACCTTGTTACGCCTTACCCTTCCTCTAAGTGATATGACTTACACAA 30037 0.184284349456675 No Hit AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA 29792 0.18278121446926326 No Hit GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA 29438 0.18060933779357455 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC 27288 0.167418561373431 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC 26286 0.16127104603715947 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG 26040 0.15976177580490117 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 26019 0.15963293566312303 No Hit GGTTACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 22881 0.14038053733456005 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA 22541 0.1382945540867234 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT 21200 0.13006719074746179 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTTATC 21159 0.1298156457087521 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA 21093 0.12941071954887792 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGC 20734 0.12720816664895626 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGATGGT 20653 0.12671121181638342 No Hit CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA 19860 0.12184596265304676 No Hit AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGCAGGGTTTACTTAT 17833 0.10940982134903236 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 17731 0.10878402637468138 No Hit AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 17354 0.10647103906752131 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC 17030 0.10448321973722992 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.779221005507732E-4 0.0 0.0 2 1.779221005507732E-4 0.0 0.0 3 1.779221005507732E-4 0.0 0.0 4 1.779221005507732E-4 0.0 0.0 5 1.840573453973516E-4 0.0 0.0 6 1.9019259024392998E-4 0.0 0.0 7 1.9019259024392998E-4 0.0 0.0 8 1.9019259024392998E-4 0.0 0.0 9 1.9019259024392998E-4 0.0 0.0 10-11 1.9632783509050835E-4 0.0 0.0 12-13 1.9632783509050835E-4 0.0 0.0 14-15 1.9632783509050835E-4 0.0 3.067622423289193E-6 16-17 1.9939545751379754E-4 0.0 6.135244846578386E-6 18-19 2.0859832478366513E-4 0.0 6.135244846578386E-6 20-21 2.1473356963024352E-4 0.0 6.135244846578386E-6 22-23 2.1473356963024352E-4 0.0 6.135244846578386E-6 24-25 2.208688144768219E-4 0.0 6.135244846578386E-6 26-27 2.270040593234003E-4 0.0 6.135244846578386E-6 28-29 2.5154503870971387E-4 0.0 6.135244846578386E-6 30-31 2.69950773249449E-4 0.0 6.135244846578386E-6 32-33 3.0062699748234094E-4 0.0 6.135244846578386E-6 34-35 3.190327320220761E-4 0.0 6.135244846578386E-6 36-37 3.405060889851004E-4 0.0 6.135244846578386E-6 38-39 3.742499356412816E-4 0.0 6.135244846578386E-6 40-41 4.2333189441390865E-4 0.0 6.135244846578386E-6 42-43 4.5707574107008977E-4 0.0 6.135244846578386E-6 44-45 5.030900774194277E-4 0.0 6.135244846578386E-6 46-47 5.797806380016576E-4 3.0676224232891934E-5 6.135244846578386E-6 48-49 6.349978416208629E-4 3.0676224232891934E-5 6.135244846578386E-6 50-51 6.932826676633577E-4 3.0676224232891934E-5 6.135244846578386E-6 52-53 8.037170749017686E-4 3.0676224232891934E-5 6.135244846578386E-6 54-55 9.417600839497823E-4 3.0676224232891934E-5 6.135244846578386E-6 56-57 0.001095141205114242 3.0676224232891934E-5 1.2270489693156772E-5 58-59 0.0012638604383951476 3.0676224232891934E-5 1.2270489693156772E-5 60-61 0.0014417825389459208 3.0676224232891934E-5 1.2270489693156772E-5 62-63 0.001702530444925502 3.0676224232891934E-5 1.2270489693156772E-5 64-65 0.0019663459733283727 3.0676224232891934E-5 1.2270489693156772E-5 66-67 0.002190282410228484 3.0676224232891934E-5 1.5338112116445964E-5 68-69 0.0024970446525574034 3.0676224232891934E-5 1.840573453973516E-5 70-71 0.0027915364051931658 3.0676224232891934E-5 1.840573453973516E-5 72-73 0.0031351101166015556 3.0676224232891934E-5 1.840573453973516E-5 74-75 0.0034848190728565234 3.0676224232891934E-5 1.840573453973516E-5 76-77 0.0038744071206142506 3.0676224232891934E-5 2.147335696302435E-5 78-79 0.004310009504721317 3.0676224232891934E-5 2.4540979386313543E-5 80-81 0.00467505657309273 3.0676224232891934E-5 2.4540979386313543E-5 82-83 0.005144402803855977 3.0676224232891934E-5 2.4540979386313543E-5 84-85 0.005607613789772646 3.0676224232891934E-5 2.4540979386313543E-5 86-87 0.006162853448387989 3.0676224232891934E-5 2.4540979386313543E-5 88-89 0.006773310310622538 3.0676224232891934E-5 2.4540979386313543E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTTGT 1534110 0.0 94.970566 5 TGTTACG 1534230 0.0 94.943306 9 CTTGTTA 1535445 0.0 94.94057 7 TTGTTAC 1535315 0.0 94.91921 8 GTTACCT 656705 0.0 94.914635 2 CCTTGTT 1539585 0.0 94.89938 6 AGATACC 315700 0.0 94.89917 1 GATACCT 346720 0.0 94.889015 2 TACCTTG 1227620 0.0 94.87965 4 TCGTACC 40990 0.0 94.79138 1 ATACCTT 349265 0.0 94.6763 3 AACCTTG 289325 0.0 94.57312 4 TTACCTT 659910 0.0 94.47884 3 GGAAACC 276220 0.0 94.41014 1 GGTTACC 653865 0.0 94.354164 1 CTACCTT 166640 0.0 94.23321 3 AAACCTT 281720 0.0 94.16032 3 GAAACCT 282565 0.0 93.99304 2 CGTACCT 42105 0.0 93.68006 2 GTACCTT 60575 0.0 93.14938 3 >>END_MODULE