FastQCFastQC Report
Sun 19 Apr 2015
000000000-AE2TW l01n03.33300000008a65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE2TW l01n03.33300000008a65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16299268
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT247824015.20460918858442No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC222168513.630581446970503No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT15772709.676937639162691No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC5845833.586559838147333No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT4874922.9908827807481906No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAGAAAAA3852912.363854622183033No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG3508432.152507707708101No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA2772951.7012727197319537No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC2661351.632803387244139No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA2637641.6182567217129014No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT2618141.6062929942620734No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC2370801.4545438482268038No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG2207601.3544166523306445No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCCAAGGTTTGTTTAA1279970.7852929346274937No Hit
GGCCACCTTGTTACGACTTTTGCCCGGTTCAAGCCAACGCGATCAAACCG1247180.7651754667755631No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA1148630.7047126288125332No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA1036800.6361021856932471No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT952590.5844372888402105No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA877410.5383125180836341No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTTAAAAAA874250.5363737807121154No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC865940.5312753922446088No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC862790.5293427901179366No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAAGTG842620.516968001262388No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT806080.4945498165929906No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC654900.4017971850024185No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC650070.39883386174152113No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCTAT629380.3861400401539505No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA616290.37810900464977937No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA582700.35750071721012255No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA571470.35061083724741504No Hit
TGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGGT570760.350175234863308No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA543460.333426016432149No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC541810.33241370103246354No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG489880.30055337454418196No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC486820.298675989621129No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG479900.29443040018729677No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACATAT469300.2879270406499237No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC409960.25152049773032753No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC408610.25069223967603943No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA351240.21549433999121922No Hit
GAAGACCTTGTTACGACTTCTCCTTCTTCTAAATTGCAAGATTCGAACAA350040.21475811060962985No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA336850.20666572265699296No Hit
GGAAACCTTGTTACGCCTTACCCTTCCTCTAAGTGATATGACTTACACAA300370.184284349456675No Hit
AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA297920.18278121446926326No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA294380.18060933779357455No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC272880.167418561373431No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC262860.16127104603715947No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG260400.15976177580490117No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC260190.15963293566312303No Hit
GGTTACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT228810.14038053733456005No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA225410.1382945540867234No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT212000.13006719074746179No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTTATC211590.1298156457087521No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA210930.12941071954887792No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGC207340.12720816664895626No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGATGGT206530.12671121181638342No Hit
CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA198600.12184596265304676No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGCAGGGTTTACTTAT178330.10940982134903236No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT177310.10878402637468138No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC173540.10647103906752131No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC170300.10448321973722992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTGT15341100.094.9705665
TGTTACG15342300.094.9433069
CTTGTTA15354450.094.940577
TTGTTAC15353150.094.919218
GTTACCT6567050.094.9146352
CCTTGTT15395850.094.899386
AGATACC3157000.094.899171
GATACCT3467200.094.8890152
TACCTTG12276200.094.879654
TCGTACC409900.094.791381
ATACCTT3492650.094.67633
AACCTTG2893250.094.573124
TTACCTT6599100.094.478843
GGAAACC2762200.094.410141
GGTTACC6538650.094.3541641
CTACCTT1666400.094.233213
AAACCTT2817200.094.160323
GAAACCT2825650.093.993042
CGTACCT421050.093.680062
GTACCTT605750.093.149383
Produced by FastQC (version 0.11.2)