##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE2TW l01n02.33200000008a68.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16299268
Sequences flagged as poor quality	0
Sequence length	12
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.353132238822013	33.0	32.0	34.0	16.0	34.0
2	30.613643876522552	33.0	32.0	34.0	16.0	34.0
3	30.768461687972735	33.0	32.0	34.0	16.0	35.0
4	30.754263381643888	33.0	32.0	34.0	16.0	35.0
5	30.68701312230709	33.0	32.0	34.0	16.0	35.0
6	33.297254146627935	37.0	33.0	37.0	18.0	37.0
7	32.74434422453818	36.0	32.0	37.0	16.0	37.0
8	32.520964561107895	34.0	32.0	37.0	16.0	37.0
9	33.15105641553964	37.0	33.0	37.0	18.0	37.0
10	32.79287701754459	35.0	33.0	37.0	18.0	37.0
11	32.64331477953488	35.0	33.0	37.0	18.0	37.0
12	32.15358106879401	34.0	32.0	37.0	16.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.28022014826720465
1101	2	-0.0214973842424655
1101	3	0.12263923789052811
1101	4	0.21686873705063192
1101	5	0.2638133284166422
1101	6	0.32406998316554336
1101	7	0.4158511461320771
1101	8	0.3343933889972064
1101	9	0.29251561990814423
1101	10	0.43913037180218595
1101	11	0.36161019900072233
1101	12	0.5088716276278333
1102	1	0.35250483327953575
1102	2	0.32995748323955
1102	3	0.34435259588227396
1102	4	0.31910202462432125
1102	5	0.3967610051386572
1102	6	0.3261546559322923
1102	7	0.4719341758490927
1102	8	0.37020363868429484
1102	9	0.34214222770712865
1102	10	0.4769078630913839
1102	11	0.38968832113609153
1102	12	0.3452195939408753
1103	1	0.35460832276429954
1103	2	0.3363931967390137
1103	3	0.42468261035551436
1103	4	0.3496757986724077
1103	5	0.24630452297520478
1103	6	0.37528565647554757
1103	7	0.39553557997406585
1103	8	0.389634401850671
1103	9	0.4020747616769569
1103	10	0.3483413733374192
1103	11	0.3849633341085621
1103	12	0.5557044805356668
2112	1	0.2842414959503685
2112	2	0.36825954776754344
2112	3	0.30750840776770616
2112	4	0.3407809920541851
2112	5	0.44983408459432184
2112	6	0.40746312133462936
2112	7	0.3977300574921685
2112	8	0.3803959651572626
2112	9	0.4171905689344655
2112	10	0.2877481876771526
2112	11	0.3964650412713411
2112	12	0.6371024300657453
1113	1	0.17560944312975124
1113	2	0.2734174907524185
1113	3	0.32830190281095284
1113	4	0.4073703440206309
1113	5	0.2923675891541855
1113	6	0.44035884764466005
1113	7	0.5003587529992686
1113	8	0.26223856107737475
1113	9	0.3633310148417692
1113	10	0.381101259381488
1113	11	0.4435041096577308
1113	12	0.6487819376828909
2113	1	0.1892345931061108
2113	2	0.2109261433403944
2113	3	0.3371873433162449
2113	4	0.2555538409885365
2113	5	0.27657611140877236
2113	6	0.38775650205072765
2113	7	0.17875359699194604
2113	8	0.16238009662405517
2113	9	0.2808858231833753
2113	10	0.2746733780146826
2113	11	-0.056861587325009566
2113	12	0.48157854240296416
1112	1	0.2767461542191505
1112	2	0.216035917106435
1112	3	0.1041657186956435
1112	4	0.16682128674586494
1112	5	0.10247220148744418
1112	6	0.19021707534746213
1112	7	0.01634584545171691
1112	8	0.02178521668525235
1112	9	0.12467199548095209
1112	10	0.26808885268763305
1112	11	-0.2558227122869283
1112	12	0.26408038389357813
2114	1	0.2659974754125294
2114	2	0.24256410192572275
2114	3	0.28101238468343936
2114	4	0.14214574563961335
2114	5	0.17829698241838798
2114	6	0.23116826949819114
2114	7	0.24948828881357343
2114	8	0.23903938001337366
2114	9	0.1984561488639187
2114	10	0.4316296599230043
2114	11	0.08172533647250191
2114	12	0.4933783324116163
1114	1	0.34069666799825526
1114	2	-0.018687078914730648
1114	3	0.08621823966453945
1114	4	0.1841062170054002
1114	5	0.24309819640421537
1114	6	0.2752756271864527
1114	7	0.31094032944617567
1114	8	0.28374519582798996
1114	9	0.2817250791393846
1114	10	0.42714023019879477
1114	11	0.23247239343227477
1114	12	0.2750884802154303
1109	1	0.21627828266999316
1109	2	0.1506271263251513
1109	3	0.13616685856593236
1109	4	0.12484116873937978
1109	5	0.13696005433133607
1109	6	0.1533153791569788
1109	7	0.10330634943779415
1109	8	0.10103574546702276
1109	9	0.1169496943257684
1109	10	0.3439255385747657
1109	11	0.2138960176030409
1109	12	0.07395522395529497
1108	1	0.20951215661022005
1108	2	0.013152618701585794
1108	3	-0.06567920758651624
1108	4	-0.09906578413643885
1108	5	0.003991524181611084
1108	6	-0.14705212208333762
1108	7	-0.25059731595231227
1108	8	-0.1949084089057962
1108	9	-0.11518243400504247
1108	10	-0.0802964788532492
1108	11	-0.06548119021503851
1108	12	-0.3427293177170121
1111	1	0.5298736408792131
1111	2	0.5008768757702349
1111	3	0.4612518587922736
1111	4	0.5031132236598452
1111	5	0.26246913364342106
1111	6	0.361405815785524
1111	7	0.44163877574619903
1111	8	0.4834227020007589
1111	9	0.41507597585969336
1111	10	0.4964517426628561
1111	11	0.4360436975191462
1111	12	0.4723725551225968
1110	1	-0.02364243763157603
1110	2	-0.08960115110143008
1110	3	-0.07264428417684954
1110	4	-0.13019587245924313
1110	5	-0.21567809100362823
1110	6	-0.15855549090809973
1110	7	-0.1713098472846255
1110	8	-0.18620089760360514
1110	9	-0.22720310181667003
1110	10	-0.08644701324448789
1110	11	-0.05436203985795629
1110	12	-0.022384424403007586
1105	1	0.27109035251772085
1105	2	0.21549841853459029
1105	3	0.22116376265923776
1105	4	0.16277171501467436
1105	5	-0.006696588613028354
1105	6	0.025990663525377045
1105	7	0.03871459948182121
1105	8	0.04382644672101321
1105	9	0.1153002294573966
1105	10	0.020402010419765304
1105	11	0.2722557766785201
1105	12	0.1132454457224128
1104	1	-1.382627496785073
1104	2	-0.6105047745595655
1104	3	-0.742772346466392
1104	4	-0.3829599280298055
1104	5	-0.280774563244627
1104	6	-0.11282606395067063
1104	7	-0.2652376971056185
1104	8	-0.023514169912623117
1104	9	-0.14690711896486164
1104	10	0.1709799591552823
1104	11	-0.14169444461093406
1104	12	-0.049052146092797955
1107	1	-0.08957767727462951
1107	2	-0.07076575588747858
1107	3	-0.013183739128145078
1107	4	-0.11702525185692636
1107	5	-0.08065621375799736
1107	6	0.009499583144773283
1107	7	-0.11295266096492895
1107	8	0.05819227976878949
1107	9	-0.12846574065839178
1107	10	0.22867979962995122
1107	11	0.05904546725111004
1107	12	-0.06297770914751766
1106	1	0.15391118070517606
1106	2	0.0922948910285939
1106	3	0.10236374534481385
1106	4	0.0744296415697363
1106	5	-0.037808536553264105
1106	6	-0.062437018248992615
1106	7	-0.1321699477679843
1106	8	0.02101282002657001
1106	9	-0.1204614076489321
1106	10	-0.025515550022348066
1106	11	0.07401165619815941
1106	12	0.017495389705160846
2103	1	0.47572542042028587
2103	2	0.48106812404268595
2103	3	0.3519246824900506
2103	4	0.4214948736198849
2103	5	0.44049954118273504
2103	6	0.31368647121961857
2103	7	0.19699801663801253
2103	8	0.3048536247931324
2103	9	0.46550337085518123
2103	10	0.17341488221443768
2103	11	0.32133383476320887
2103	12	0.24929032782880256
2102	1	-0.27874356557114766
2102	2	-0.18897913411924705
2102	3	-0.11343884411064664
2102	4	-0.04435897403390143
2102	5	-0.19249947903531606
2102	6	-0.12987419577011394
2102	7	-0.2943928392008175
2102	8	-0.30875822958934407
2102	9	-0.10056333378005178
2102	10	-0.2677701893856437
2102	11	-0.09877416360664171
2102	12	-0.588583616084648
2101	1	0.12373728527612826
2101	2	0.23814102875511622
2101	3	0.3463563430848957
2101	4	0.09513452752550222
2101	5	0.3057838349286257
2101	6	0.3315082245689993
2101	7	0.29865981287418464
2101	8	0.18921130859911273
2101	9	0.4190187646227912
2101	10	0.1675679516339983
2101	11	0.14259773799679465
2101	12	-0.261374393776471
2107	1	-0.15356332884859114
2107	2	-0.1358110273963291
2107	3	-0.12692982468676917
2107	4	-0.17682291561917296
2107	5	-0.07469445323937407
2107	6	-0.21954287279746154
2107	7	-0.03342424681169831
2107	8	-0.12926030965038393
2107	9	0.0040643073269706065
2107	10	-0.47122478544995516
2107	11	-0.236270939478608
2107	12	-0.4599104259063971
2106	1	-0.5030558272895043
2106	2	-0.3753588262195464
2106	3	-0.42470712923762477
2106	4	-0.4237007615877104
2106	5	-0.4186452495225197
2106	6	-0.5671602119725847
2106	7	-0.2136026682925234
2106	8	-0.28442957875895303
2106	9	-0.3151567435220457
2106	10	-0.5373803434168707
2106	11	-0.4422742040846117
2106	12	-0.8256637280823398
2105	1	-0.08713778485128643
2105	2	-0.09939066136996644
2105	3	-0.3337185234053237
2105	4	-0.3584178947681984
2105	5	-0.2337194873561259
2105	6	-0.4752270534527909
2105	7	-0.13173693494029948
2105	8	-0.2891623452654599
2105	9	-0.20530165056786132
2105	10	-0.6825136039703068
2105	11	-0.3797329864654273
2105	12	-0.2768494382482416
2104	1	0.2930279986700306
2104	2	0.23076784703312825
2104	3	0.3442828715744426
2104	4	0.2506756314466756
2104	5	0.011012100909933764
2104	6	0.10295627150065911
2104	7	0.02659766788506346
2104	8	0.05885514414846682
2104	9	0.05137956088572082
2104	10	-0.371430054253608
2104	11	-0.1604362221318425
2104	12	0.005817574017505933
2111	1	-0.5114974439922548
2111	2	-0.6955196682454314
2111	3	-0.5675110705363338
2111	4	-0.6349218122921343
2111	5	-0.3867045253354391
2111	6	-0.6548062754833168
2111	7	-0.5980897296037853
2111	8	-0.7526783695976995
2111	9	-0.6325015779879308
2111	10	-0.5340323692950619
2111	11	-0.7324121338081575
2111	12	-0.6711567477070552
2110	1	-0.027061314221768384
2110	2	0.049540431924221195
2110	3	-0.019776164286408715
2110	4	0.07348633292927786
2110	5	-0.11368612353928143
2110	6	-0.09401030503472896
2110	7	-0.13632762710193447
2110	8	-0.17207796199465264
2110	9	-0.16324708294055768
2110	10	0.012140021158657532
2110	11	-0.004980728204181162
2110	12	-0.08955010049986356
2109	1	-0.684350162520353
2109	2	-1.0484660332948685
2109	3	-0.8244368050637974
2109	4	-1.1035707714553986
2109	5	-0.8237590806885926
2109	6	-0.7772398732339809
2109	7	-1.0297631920872483
2109	8	-0.7390148794452962
2109	9	-1.1287982120152478
2109	10	-1.0062098108317272
2109	11	-0.841125958255045
2109	12	-0.8911468045161506
2108	1	-0.491318116355302
2108	2	-0.5949397476353333
2108	3	-0.9947806248938882
2108	4	-0.6173321350677199
2108	5	-0.7449178192864458
2108	6	-0.8573806646011306
2108	7	-0.6732482880997068
2108	8	-0.6242207657186469
2108	9	-1.0064967391623298
2108	10	-0.8855028828403881
2108	11	-0.3393836127589154
2108	12	-0.6006034729469718
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	31.0
15	1946.0
16	50643.0
17	134382.0
18	234544.0
19	312468.0
20	307884.0
21	362208.0
22	362279.0
23	313478.0
24	304269.0
25	298563.0
26	282043.0
27	279950.0
28	301640.0
29	322614.0
30	388295.0
31	483167.0
32	726066.0
33	1594519.0
34	4069031.0
35	4587904.0
36	581344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.978967399026754	45.50303731431375	31.53660029395185	10.981394992707648
2	32.21714619331371	32.74849520849648	23.422076377908503	11.612282220281303
3	17.466986861005047	14.58785756513728	27.692329496023994	40.25282607783368
4	36.13437732295708	19.046205019759167	22.09489407745182	22.724523579831928
5	34.7396112966307	28.242380470238327	13.522687860748583	23.495320372382388
6	22.80110370600692	51.946756136533246	19.482058948904946	5.770081208554887
7	22.12271877011402	10.721635518946956	29.337619906465733	37.818025804473294
8	13.459475603444277	33.248799884755556	25.265711319060465	28.026013192739697
9	9.978233378333309	31.594731738873183	33.652296532580486	24.774738350213028
10	26.01544437455719	32.266399938942044	6.9609015570515185	34.75725412944925
11	32.0687775671889	19.959160129154267	1.1224000979675897	46.849662205689235
12	19.64084522077924	27.498173537609173	25.438314162329252	27.42266707928233
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	310.0
2	310.0
3	310.0
4	999.0
5	1688.0
6	1688.0
7	1688.0
8	1688.0
9	1688.0
10	1688.0
11	1688.0
12	1688.0
13	5447.0
14	9206.0
15	9206.0
16	9206.0
17	9206.0
18	9206.0
19	9206.0
20	9206.0
21	26207.5
22	43209.0
23	43209.0
24	43209.0
25	43209.0
26	43209.0
27	43209.0
28	43209.0
29	136930.5
30	230652.0
31	230652.0
32	230652.0
33	230652.0
34	230652.0
35	230652.0
36	230652.0
37	230652.0
38	3010817.5
39	5790983.0
40	5790983.0
41	5790983.0
42	5790983.0
43	5790983.0
44	5790983.0
45	5790983.0
46	5553364.5
47	5315746.0
48	5315746.0
49	5315746.0
50	5315746.0
51	5315746.0
52	5315746.0
53	5315746.0
54	4951964.0
55	4588182.0
56	4588182.0
57	4588182.0
58	4588182.0
59	4588182.0
60	4588182.0
61	4588182.0
62	4588182.0
63	2421094.5
64	254007.0
65	254007.0
66	254007.0
67	254007.0
68	254007.0
69	254007.0
70	254007.0
71	153992.5
72	53978.0
73	53978.0
74	53978.0
75	53978.0
76	53978.0
77	53978.0
78	53978.0
79	31701.0
80	9424.0
81	9424.0
82	9424.0
83	9424.0
84	9424.0
85	9424.0
86	9424.0
87	9424.0
88	5389.5
89	1355.0
90	1355.0
91	1355.0
92	1355.0
93	1355.0
94	1355.0
95	1355.0
96	941.5
97	528.0
98	528.0
99	528.0
100	528.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	6.135244846578386E-6
6	0.0
7	6.135244846578386E-6
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.6299268E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.3563061281461897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.11791275711981	1.6050665526612895
2	12.252448985455148	0.5774104125845306
3	4.835646219447953	0.341827884612965
4	2.6388887092708555	0.24872118548602828
5	1.8409786743545737	0.21689546660840653
6	1.484667423847926	0.20989985694431307
7	0.965408480286336	0.15923585427840956
8	0.9399685269899007	0.177188288032868
9	0.6413546828172616	0.1360105172493812
>10	5.023091976160187	2.3711346291087874
>50	0.6378331802684734	1.0428332532854556
>100	0.4652136831827149	2.1435266868428395
>500	0.07669165230009714	1.2804094777778259
>1k	0.07159806481426484	3.1141957336381623
>5k	0.0025928074013745355	0.3927458626846966
>10k+	0.005704176283023899	85.98289833820407
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAGAACCTCCA	1432761	8.790339541628494	No Hit
AGCAGTCGCGAT	1095557	6.721510438382877	No Hit
AATCAGTCTCGT	997269	6.1184894929023805	No Hit
TTCAGACTACCA	956183	5.866416823135861	No Hit
GAGTGAGTACAA	883774	5.422169879039966	No Hit
ATTTCGACATGC	860297	5.278132735776846	No Hit
TGCCCATTTAAG	838741	5.145881397864002	No Hit
GCGGATGTGACT	791674	4.857113828670098	No Hit
CAGGGTGATACC	785288	4.817934155079848	No Hit
ATCGAATGCGGC	767667	4.70982500563829	No Hit
AATCCGTACAGC	766889	4.705051785147652	No Hit
AACGGATAAACG	724231	4.443334510482312	No Hit
AGTTCAGACGCT	700095	4.295254240865296	No Hit
ACCGTGCATAGG	656196	4.025923127345351	No Hit
CTTATCAAGGCG	609279	3.738075844878433	No Hit
TAATGACCACGC	565642	3.470352165508291	No Hit
TGCCTACAAGCA	551353	3.382685651895533	No Hit
TGAAAACCTCCA	27957	0.17152304017579192	No Hit
TGAGAACCTCAA	20722	0.12713454371079733	No Hit
AGCAGTCGCGCT	18330	0.11245903803778182	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAG	225	0.0	6.0000005	5
GAAATCG	55	0.009160763	6.0000005	2
GGGTGCT	275	0.0	6.0000005	3
GACAGAG	55	0.009160763	6.0000005	1
GTCGCAT	55	0.009160763	6.0000005	5
GTCGCAC	55	0.009160763	6.0000005	5
GTCGAGC	55	0.009160763	6.0000005	5
AGCCCTC	55	0.009160763	6.0000005	1
TAGAATG	225	0.0	6.0000005	2
CATGACC	110	1.8153878E-7	6.0000005	2
TAGAAGC	55	0.009160763	6.0000005	5
GATCCTA	55	0.009160763	6.0000005	4
GGCAAAG	110	1.8153878E-7	6.0000005	5
CCTACGA	55	0.009160763	6.0000005	3
CGATTGC	345	0.0	6.0000005	3
GACCCCA	55	0.009160763	6.0000005	5
GACTTGC	275	0.0	6.0000005	6
AACGGAC	440	0.0	6.0000005	1
GGAAGAT	55	0.009160763	6.0000005	3
GCACAGC	1010	0.0	6.0000005	6
>>END_MODULE